Antileishmanial Activity of Liposomal Clarithromycin against Leishmania Major Promastigotes | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 14، دوره 15، شماره 6، بهمن 2012، صفحه 1210-1214 اصل مقاله (340.59 K) | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2012.4942 | ||
| نویسندگان | ||
| Ameneh Sazgarnia1؛ Naghmeh Zabolinejad2؛ Pouran Layegh* 2؛ Omid Rajabi3؛ Fariba Berenji4؛ Zari Javidi5؛ Roshanak Salari6 | ||
| 1Department and Research Centre of Medical Physics, Avicenna Research Institute, Mashhad University of Medical Sciences. | ||
| 2Dermatology, Research Centre for Cutaneous Leishmaniasis, Qaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 3Department of Medicinal Chemistry. Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 4Parasitology, Emam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 5Dermatology, Research Centre for Cutaneous Leishmaniasis, Emam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 6Department and of Pharmaceutical Control, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| چکیده | ||
| Objective(s) Cutaneous leishmaniasis is a common parasitic disease which is endemic in some parts of the world. In vitro and in vivo studies have shown azithromycin efficacy on some Leishmania species. Because of structural similarity between clarithromycin and azithromycin and efficacy of clarithromycin against intracellular organisms and due to the absence of previous studies in this respect, we decided to evaluate the efficacy of clarithromycin against promastigotes of L. major in vitro. Materials and Method First, liposomal and non- liposomal clarithromycin were prepared, then both forms of the drug were incubated with promastigotes for 24 hr in NNN culture media without red phenol in the presence of 5% FCS with different concentrations as follows: 20, 40, 80, 100, 200 and 500 μg/ml. Results According to the results, clarithromycin in both liposomal and non- liposomal forms has in vitro activity against the promastigotes of L. major. The concentration of drug that killed 50% of parasites (ED 50) was 169 and 253.6 μg/ml for liposomal and non- liposomal forms, respectively which shows that lower concentrations of liposomal drug are required to have the same effect as non- liposomal drug and the liposomal form of the drug is more effective than non- liposomal form. Conclusion Clarithromycin in both liposomal and non- liposomal forms has in vitro activity against the promastigotes of L. major. | ||
| کلیدواژهها | ||
| Clarithromycin؛ Leishmania major؛ Liposome؛ Promastigote | ||
| مراجع | ||
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1. Mauel J. Macrophage parasite interactions in leishmania infections. J Leukoc Biol 1990; 47:187-193.
2. Croft SL, Yardiey V. Chemotherapy of leishmaniasis. Curr Pharm Des 2002; 8:319-342.
3. Krolewiecki A, Leon S, Scott P, Abraham D. Activity of azithromycin against Leishmania major in vitro and in vivo. Am J Trop Med Hyg 2002; 67:273-277.
4. de Oliveira-Silva F,de Morais-Teixeira E, Rabello A. Antileishmanial activity of azithromycin against leishmania (Leishmania) amazonensis, Leishmania (Viannia) braziliensis, and Leishmania (Leishmania) chagasi. Am J Trop Med Hyg 2008; 78:745–749.
5. Fraschini F, Scaglione F, Demartini G. Clarithromycin clinical pharmacokinetics. Clin Pharmacokinet 1993; 25:189-204.
6. Araujo FG, Prokocimer P, Lin T, Remington JS. Activity of clarithromycin alone or in combination with other drugs for treatment of murine toxoplasmosis. Antimicrob Agents Chemother 1992; 36:2454-2457. 7. Franck N, Cabié A, Villette B, Amor B, Lessana-Leibowitch M, Escande JP. Treatment of Mycobacterium chelonae- induced skin infection with clarithromycin. J Am Acad Dermatol 1993; 28:1019-1020. 8. Wharton JR, Wilson PL, Kincannon JM. Erythrasma treated with single-dose clarithromycin. Arch Dermatol 1998; 134:671-672.
9. Torresani C. Clarithromycin: a new perspective in rosacea treatment. Int J Dermatol 1998; 37:347-349.
10. Torresani C, Pavesi A, Manara GC. Clarithromycin versus doxycycline in treatment of rosacea. Int J Dermatol 1997; 36:942-946.
11. Hatziantoniou S, Nezis IP, MargaritisLH, Demetzos C. Visualisation of liposomes prepared from skin and stratum corneum lipids by transmission electron microscopy. Micron 2007; 38: 777–781.
12. Kirby C, Gregoriadis G. Dehydration-Rehydration Vesicles: A simple method for high yield drug entrapment in liposomes. Nat Biotechnol 1984; 2:979-984.
13. Jaafari MR, Bavarsad N, Bazzaz BS, Samiei A, Soroush D, Ghorbani S, et al. Effect of topical liposomes containing paromomycin sulfate in the course of Leishmania major infection in susceptible BALB/c mice. Antimicrob Agents Chemother 2009; 53:2259-2265.
14. Dagci H, Akisu Ç, Üner A, Aksoy Ü, Üstun Ş. In vivo and in vitro effect of clarithromycin on Toxoplasma gondii. Turkish Journal of Infection? 2002; 17:325-328.
15. Hardman JG, Limbird LE, Gilman AG. Goodman & Gilman’s The Pharmacological Basis of Therapeutics.10th ed. New York: Mc Graw-Hill Press; 2001.p.1252.
16. Reynolds JEF, Parfitt K, Parsons AV, Sweetman SC. Anticaterial agents. In: Reynolds JEF, Parfitt K, Parsons AV, Sweetman SC, editors. Martindale the Extra Pharmacopoeia. 31st ed. London: Royal Pharmaceutical Society of Great Britain; 1996.p.210-211.
17. Beaman M, McCabe RE, Wong S, Remington JS. Toxoplasma gondii. In: Mandell GL, Bennett JE, Dolin P, editors. Mandell, Douglas and Bennett’s Principles and Practice of Infectious Diseases. 7th ed. New York: Churchill Livingstone; 2010.p. 2455-2475.
18. Haverkos HW. Assessment of therapy for toxoplasma encephalitis. The TE Study Group. Am J Med 1987; 82:907-914.
19. Derouin F, Chastang C. Activity in vitro against Toxoplasma gondii of azithromycin and clarithromycin alone and with prymethamine. J Antimicrob Chemother 1990; 25:708-711. | ||
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