Quantitative Analysis of GABAA Gamma Receptor Subunits in the Developing Embryonic Chick Forebrain | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 14، دوره 15، شماره 5، آذر 2012، صفحه 1097-1101 اصل مقاله (136.73 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2012.4925 | ||
| نویسندگان | ||
| Sayed Rasul Zaker1؛ Abolghasem Esmaeili* 1، 2؛ Majid Bouzari1؛ Elham Shirani1، 2 | ||
| 1Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran | ||
| 2Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran | ||
| چکیده | ||
| Objective(s) In this study we investigated the expression of GABAA receptor subunits during brain development. These receptors may change in the embryonic chick forebrain. Materials and Methodes The expression levels of four types of GABAA receptor gamma subunits (γ1, γ2, γ3 and γ4) were quantified in the embryonic chick forebrain at 32 hr, 3, 7, 14, and 20 days of incubation and day one after hatching. The expression level of mRNA in the forebrain of embryonic chicken was measured using real-time RT-PCR. Results The expression level of each subunit increased gradually with development and reached a plateau on 20th day of embryonic development. A reduction was observed on day one after hatching in all gamma subunits. Conclusion This may explain the different physiological and pharmacological function of GABA receptor gamma subunits before and after hatching. | ||
| کلیدواژهها | ||
| Developmental expression؛ Embryonic forebrain؛ GABAA Receptor subunit؛ Real-time RT-PCR | ||
| مراجع | ||
|
1. Schousboe A, Waagepetersen HS. Gamma-aminobutyric acid (GABA). Encyclopedia of neuroscience. Oxford: Academic Press; 2009. p.511–515.
2. Snyder SH, Ferris CD. Novel neurotransmitters and their neuropsychiatric relevance. Am J Psychiatr 2000; 157:1738.
3. Macdonald RL, OlsenR. GABA receptor channels. Physiology and Pathology of Chloride Transporters and Channels in the Nervous System: From Molecules to Diseases, 2009: p. 257.
4. Davies M, Bateson AN, Dunn SMJ. Molecular biology of the GABA A receptor: functional domains implicated by mutational analysis. Front Biosci 1996; 1:214-233.
5. Sarto-Jackson I, Sieghart W. Assembly of GABAA receptors (Review). Mol Mem Biol 2008; 25:302-310.
6. Florey E. GABA: history and perspectives.Can J Physiol Pharmacol 1991; 69:1049.
7. Olsen RW, Sieghart W. GABAA receptors: subtypes provide diversity of function and pharmacology. Neuropharmacology 2009; 56:141-148.
8. Möhler H, Fritschy JM. GABAB receptors make it to the top--as dimers. Trends Pharmacol Sci 1999; 20:87.
9. Rodríguez Gil DJ, Vacotto M, Rapacioli M, Scicolone G, Flores V, Fiszer de Plazas. Development and localisation of GABAA receptor 1, 2, 2 and 2 subunit mRNA in the chick optic tectum. J Neurosci Res 2005; 81:469-480.
10. Burd GD, Rubel EW. Development of GABA immunoreactivity in brainstem auditory nuclei of the chick: ontogeny of gradients in terminal staining. J Comp Neurol 1989; 284:504-518.
11. Enomoto K, Kataoka H, Hirota A. Semiquantitative Analysis of the erpression of GABA-A receptor subunits in the developing embryonic Chick Brain Stem. Japan J Physiol 2001; 51: 53-61.
12. Mehta AK, Ticku MK. An update on GABAA receptors. Brain Res Rev 1999; 29:196-217.
13. Ben-Ari Y. Excitatory actions of GABA during development: the nature of the nurture. Nat Rev Neurosci 2002; 3:728-739.
14. Laurie D, Wisden W, Seeburg P. The distribution of thirteen GABAA receptor subunit mRNAs in the rat brain. III. Embryonic and postnatal development. J Neurosci 1992; 12:4151. | ||
|
آمار تعداد مشاهده مقاله: 1,290 تعداد دریافت فایل اصل مقاله: 611 |
||