The Effect of Prenatal Exposure to Restraint Stress on Hippocampal Granule Neurons of Adult Rat Offspring | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 9، دوره 15، شماره 5، آذر 2012، صفحه 1060-1067 اصل مقاله (307.45 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2012.4919 | ||
| نویسندگان | ||
| Mohammad Hosseini-sharifabad* 1؛ Ebrahim Esfandiari2؛ Ali Hosseini-sharifabad3 | ||
| 1Department of Cell Biology and Anatomy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran | ||
| 2Department of Biomedical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| 3Department of Pharmacology and Toxicology, Pharmacy Faculty, Shahid Sadoughi University of Medical Sciences, yazd, Iran | ||
| چکیده | ||
| Objective(s) It is well known that prenatal stresses (PS) induce a variety of neurobiological and behavioral alterations, some of them involving the hippocampal formation. This study aimed to determine whether restraint stress influences the neuronal volume and number of granule cells in the hippocampus of adult rat offspring. Materials and Methods Ten Wistar pregnant rats were randomly divided: stressed and control groups. Pregnant dams in the stressed group were placed in a Plexiglas restraint tube for 1 hr daily from days 15-21 of gestation. Neuroendocrinological consequences of prenatal stress exposure were evaluated in the male offspring on postnatal day 60. The total numbers and the individual volume of granule cells in the hippocampus were also estimated with the optical fractionator and the rotator methods, respectively. Results Prenatally stressed rats exhibited prolonged elevation in plasma glucocorticoid levels following acute exposure to restraint stress. Data also indicated that there is a decrease in neuronal volume of hippocampal granule cells in prenatally stressed compared with their controls (625±64.1 µm3 vs. 741±80.6 µm3). There was no significant difference in the total number of granule cells between prenatally stressed and control animals. Conclusion The present study indicated that exposure of pregnant female during last week of pregnancy leads to a decline in neuronal size in hippocampus of adult male rats without neuronal loss. The present results may provide a basis for the understanding of the reported disturbances in behavior and learning of PS offspring. | ||
| کلیدواژهها | ||
| Dentate gyrus؛ Hippocampus؛ Neuron؛ Number؛ Prenatal stress؛ Volume | ||
| مراجع | ||
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