Prevalence of PER-1- type Extended-Spectrum Beta-Lactamaes in Clinical Strains of Pseudomonas aeruginosa Isolated from Tabriz, Iran | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 10، دوره 15، شماره 1، فروردین 2012، صفحه 678-682 اصل مقاله (211.33 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2012.4837 | ||
| نویسندگان | ||
| Mohammad Taghi Akhi* 1؛ Yones Khalili1؛ Reza Ghottaslou1؛ Mohammad Aghazadeh1؛ Mohammad hosein Seroush Bar Haghi1؛ Saber Yousefi2 | ||
| 1Department of Microbiology, Faculty of Medicine, and *Research Center of Infectious Diseases and Tropical Medicine, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 2Department of Clinical Laboratory Sciences, Faculty of Paramedicine, Urmia University of Medical Science | ||
| چکیده | ||
| Objective(s) The aim of this study was to investigate the presence of PER-1-type ESBLs in drug resistant Pseudomonas aeruginosa isolates. Materials and Methods During one-year period (2008-2009), following isolation and identification of 56 P. aeruginosa, the E-test method was performed for determination of minimal inhibitory concentration of ceftazidim. The isolates that they had MIC≥16 µg/ml against ceftazidim were used for determination of ESBL-producing by combined disk test (CDT) and double disk synergy test (DDST) methods. Bla PER-1genewas investigated by PCR.P. aeruginosa KOAS was used as positive control. Results Twenty-nine (51.78%) out of fifty six isolates had MIC≥16 µg/ml to ceftazidime, twenty two (75.86%) of them were ESBL producers. Some isolates (27.5%) contained bla PER-1 gene. Conclusion PER-1-type ESBLs producing P.aeruginosa has not been reported previously in Tabriz but there has been a rather high prevalence of it. | ||
| کلیدواژهها | ||
| Combined Disk Test؛ Double Disk Synergy Test؛ Extended- Spectrum Beta- Lactamase؛ PER-1gene؛ Pseudomonas aeruginosa | ||
| مراجع | ||
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