Molecular Identification of Rare Clinical Mycobacteria by Application of 16S-23S Spacer Region Sequencing | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 8، دوره 15، شماره 1، فروردین 2012، صفحه 661-668 اصل مقاله (119.61 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2012.4835 | ||
| نویسندگان | ||
| Hasan Shojaei* 1؛ Abodolrazagh Hashemi1؛ Parvin Heidarieh2؛ Fazel Pourahmad3؛ Abass Daei Naser4، 5 | ||
| 1Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| 2Infectious and Tropical Disease Research Center, Ahvaz Jundishapur University of Medical Sciences, Iran | ||
| 3Microbiology Group, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran | ||
| 4School of Veterinary Medicine, Ilam University, Ilam, Iran | ||
| 5Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| چکیده | ||
| Objective(s) In addition to several molecular methods and in particular 16S rDNA analysis, the application of a more discriminatory genetic marker, i.e., 16S-23S internal transcribed spacer gene sequence has had a great impact on identification and classification of mycobacteria. In the current study we aimed to apply this sequencing power to conclusive identification of some Iranian clinical strains of mycobacteria. Materials and Methods The test strains consisted of nineteen mycobacterial isolates which were initially identified by the use of conventional phenotypic techniques and molecular methods and subjected to further definitive identification using the 16S-23S internal transcribed spacer gene sequencing. Results Out of 19 studied strains, 7 isolates were found to be rapidly growing and 12 isolates as slowly growing mycobacteria. With the exception of one isolate, i.e., the isolate HNTM87, which yielded a distinct ITS sequence incomparable with all previously identified mycobacteria, the remaining isolates produced the sequences similar to the established mycobacteria and were clearly identified and differentiated from closely related taxa. A phylogenetic tree based on maximum parsimony analysis of 16S-23S internal transcribed spacer gene sequences constructed showing the relatedness of Iranian clinical isolates with the closely related type species of mycobacteria. Conclusion This study showed that the 16S-23S internal transcribed spacer gene of the genus Mycobacterium exhibits a high variation which is of value for discriminating closely related taxa and could be used independently or in combination with 16S rDNA sequencing to delineate the true identity of rare mycobacterial species. | ||
| کلیدواژهها | ||
| Internal Transcribed Spacer؛ Mycobacterium؛ Sequence Analysis | ||
| مراجع | ||
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