Fallahi, Maryam, Keyhanmanesh, Rana, Khamaneh, Amir Mahdi, Ebrahimi Saadatlou, Mohammad Ali, Saadat, Saeideh, Ebrahimi, Hadi. (1394). Effect of Alpha-Hederin, the active constituent of Nigella sativa, on miRNA-126, IL-13 mRNA levels and inflammation of lungs in ovalbumin-sensitized male rats. , 6(1), 77-85. doi: 10.22038/ajp.2016.4622
Maryam Fallahi; Rana Keyhanmanesh; Amir Mahdi Khamaneh; Mohammad Ali Ebrahimi Saadatlou; Saeideh Saadat; Hadi Ebrahimi. "Effect of Alpha-Hederin, the active constituent of Nigella sativa, on miRNA-126, IL-13 mRNA levels and inflammation of lungs in ovalbumin-sensitized male rats". , 6, 1, 1394, 77-85. doi: 10.22038/ajp.2016.4622
Fallahi, Maryam, Keyhanmanesh, Rana, Khamaneh, Amir Mahdi, Ebrahimi Saadatlou, Mohammad Ali, Saadat, Saeideh, Ebrahimi, Hadi. (1394). 'Effect of Alpha-Hederin, the active constituent of Nigella sativa, on miRNA-126, IL-13 mRNA levels and inflammation of lungs in ovalbumin-sensitized male rats', , 6(1), pp. 77-85. doi: 10.22038/ajp.2016.4622
Fallahi, Maryam, Keyhanmanesh, Rana, Khamaneh, Amir Mahdi, Ebrahimi Saadatlou, Mohammad Ali, Saadat, Saeideh, Ebrahimi, Hadi. Effect of Alpha-Hederin, the active constituent of Nigella sativa, on miRNA-126, IL-13 mRNA levels and inflammation of lungs in ovalbumin-sensitized male rats. , 1394; 6(1): 77-85. doi: 10.22038/ajp.2016.4622

Effect of Alpha-Hederin, the active constituent of Nigella sativa, on miRNA-126, IL-13 mRNA levels and inflammation of lungs in ovalbumin-sensitized male rats

Avicenna Journal of Phytomedicine
مقاله 8، دوره 6، شماره 1، فروردین و اردیبهشت 2016، صفحه 77-85    اصل مقاله (489.98 K)
نوع مقاله: Original Research Article
شناسه دیجیتال (DOI): 10.22038/ajp.2016.4622
نویسندگان
Maryam Fallahi1؛ Rana Keyhanmanesh2؛ Amir Mahdi Khamaneh3؛ Mohammad Ali Ebrahimi Saadatlou4؛ Saeideh Saadat5؛ Hadi Ebrahimi* 6
1Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
2Medical Education Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3Department of molecular medicine, School of advanced medical sciences, Tabriz University of Medical Sciences, Tabriz, Iran
4Department of basic sciences, College of veterinary medicine, Tabriz branch, Islamic Azad University, Tabriz, Iran
5Department of Physiology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
6Tuberculosis and lung diseases research center, Tabriz University of Medical Sciences, Tabriz, Iran
چکیده
Objectives: In previous studies the therapeutic effects of Nigella sativa have been demonstrated on asthmatic animals. In the present study, the preventive effect of single dose of alpha-hederin, its active constituent, has been evaluated on lung inflammation and some inflammatory mediators in lungs of ovalbumin sensitized rat in order to elicit its mechanism.
Materials and methods: Forty rats were randomly grouped in 4 groups; control (C), sensitized (S), sensitized pretreated groups with thymoquinone (3 mg/kg i.p., S+TQ) and alpha-hederin (0.02 mg/kg i.p., S+AH). Levels of IL-13 mRNA and miRNA-126 in lung tissue and its pathological changes in each group were assessed.
Results: Elevated levels of miRNA-126, IL-13 mRNA and pathological changes were observed in the sensitized group compared to the control group (pConclusion: The results suggested that alpha-hederin had preventive effect on sensitized rats like thymoquinone. It may intervene in miRNA-126 expression, which consequently could interfere with IL-13 secretion pathway leading to a reduction in inflammatory responses.
کلیدواژه‌ها
Alpha-hederin؛ MiRNA-126؛ IL-13 mRNA؛ Ovalbumin؛ Thymoquinone؛ Asthma
مراجع
آمار
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