Growth inhibition and apoptosis induction of Scutellaria luteo-coerulea Bornm. & Sint. on leukemia cancer cell lines K562 and HL-60 | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 8، دوره 5، شماره 6، بهمن و اسفند 2015، صفحه 553-559 اصل مقاله (517.36 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2015.4428 | ||
| نویسندگان | ||
| Mahsa Motaez1؛ Seyed Ahmad Emami2؛ Zahra Tayarani Najjaran* 1 | ||
| 1Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad, University of Medical Sciences, Mashhad, Iran. | ||
| 2Department of Pharmacognosy, School of Pharmacy, Mashhad, University of Medical Sciences, Mashhad, Iran | ||
| چکیده | ||
| Objective: Scutellaria (Lamiaceae) has been implicated for medicinal purposes both in modern and traditional medicine. Some species of the genus Scutellaria has extensively been studied for anticancer activity. Scutellaria luteo-coerulea (S. luteo-coerulea) is one of the Iranian species of the genus Scutellaria. Materials and Methods: In the present study, cytotoxic and apoptogenic properties of CH2Cl2, EtOAc, n-BuOH, and H2O fractions of S. luteo-coerulea were investigated on K562. Moreover, HL-60. DNA fragmentation in apoptotic cells were determined by propidium iodide (PI) staining (sub-G1 peak). Results: Scutellaria luteo-coerulea inhibited the growth of malignant cells in a dose-dependent manner. Among solvent fractions of S. luteo-coerulea, the CH2Cl2 fraction was found to be the most cytotoxic one among others. Sub-G1 peak in flow cytometry histogram of treated cells suggested the induction of apoptosis in S. luteo-coerulea. Conclusion: Scutellaria luteo-coerulea could be a novel candidate for further analytical elucidation in respect to fine major components responsible for the cytotoxic effect of the plant also clinical evaluations. | ||
| کلیدواژهها | ||
| Scutellaria luteo-coerulea؛ Cytotoxicity؛ Apoptosis؛ Lamiaceae | ||
| مراجع | ||
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