The effect of intrathecal delivery of bone marrow stromal cells on hippocampal neurons in rat model of Alzheimer’s disease | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 14، دوره 18، شماره 5، مرداد 2015، صفحه 520-525 اصل مقاله (1.55 M) | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2015.4417 | ||
| نویسندگان | ||
| Mina Eftekharzadeh1؛ Maliheh Nobakht1، 2؛ Akram Alizadeh3؛ Mansooreh Soleimani1، 2، 4؛ Mahmood Hajghasem5؛ Bahareh Kordestani Shargh6؛ Nushin Karkuki Osguei7؛ Babak Behnam2، 4؛ Ali Samadikuchaksaraei* 2، 8، 9 | ||
| 1Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran | ||
| 2Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran | ||
| 3Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran | ||
| 4Department of Medical Genetics and Molecular Biology, Iran University of Medical Sciences, Tehran, Iran | ||
| 5Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran | ||
| 6Department of Basic Sciences, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran | ||
| 7Eposcience Millennium Institute, Tehran, Iran | ||
| 8Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran | ||
| 9Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran | ||
| چکیده | ||
| Objective(s): Intracerebral injection of bone marrow stromal cells (BMSCs) is being investigated as a therapeutic tool to prevent Alzheimer's disease (AD). Our aim was to investigate the effects of BMSCs by intrathecal injection in AD rat model. Materials and Methods: BMSCs were obtained from the bone marrow of Wistar rat and transplanted into AD rat model via intrathecal injection. The rat model had received an injection of β amyloid into the hippocampus for histological and immunohistochemical studies. Results: Histological examination of the brains in transplanted rats compared to controls demonstrated the migration of BrdU-labeled BMSCs from the site of delivery, confirmed the differentiation of BMSCs transplanted cells into the cholinergic neurons, and increased number of healthy and decreased number of dark neurons. Conclusion: Our results showed that BMSCs intratechal administration could be a promising method for treatment ofAlzheimer’s disease in rat model. | ||
| کلیدواژهها | ||
| Alzheimer’s disease؛ Bone marrow stromal cells؛ Intrathecal delivery؛ Rat model | ||
| مراجع | ||
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