Ferulic acid protects PC12 neurons against hypoxia by inhibiting the p-MAPKs and COX-2 pathways | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 8، دوره 18، شماره 5، مرداد 2015، صفحه 478-484 اصل مقاله (1.57 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2015.4411 | ||
| نویسندگان | ||
| Wen-Chieh Lin1؛ Yu-Fen Peng2؛ Chien-Wei Hou* 3 | ||
| 1Department of Optometry, Yuanpei University, Hsinchu, Taiwan | ||
| 2Department of Physical Education Office, Yuanpei University, Hsinchu, Taiwan | ||
| 3Department of Biotechnology, Yuanpei University, Hsinchu, Taiwan | ||
| چکیده | ||
| Objective(s):Hypoxia induces cellular oxidative stress that is associated with neurodegenerative diseases. Here, the protective effects of ferulic acid (FA) on hypoxia-induced neurotoxicity in PC12 cells were evaluated. Materials and Methods:We investigated the effect of FA on PC12 cells subjected to hypoxia stress, in vitro. Results:FA increased cell viability, prevented membrane damage (LDH release), scavenged free radicals, increased superoxide dismutase (SOD) activity, and attenuated the elevation of intracellular free Ca2+, lipid peroxidation, apoptosis (evaluated by TUNEL staining) and PGE2 production in hypoxia-stressed PC12 cells. MAPKs were activated during hypoxia. FA reduced p-p38 MAPK, caspase-3, and COX-2 activation which correlated well with diminished LDH release in PC12 cells under hypoxia. Furthermore, FA reduced lipid peroxidation in PC12 cells subjected to hypoxia. Conclusion:Taken together, these results indicate that FA antioxidant effects could partly be involved in inhibition of p38 MAPK pathway and apoptosis through scavenging ROS in hypoxia-stressed PC12 cells. | ||
| کلیدواژهها | ||
| Caspase-3؛ Ferulic acid؛ Hypoxia؛ MAPKs؛ ROS | ||
| مراجع | ||
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