Activation of calcium/calmodulin-dependent kinase II following bovine rotavirus enterotoxin NSP4 expression | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 12، دوره 18، شماره 4، تیر 2015، صفحه 393-397 اصل مقاله (1.6 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2015.4289 | ||
| نویسندگان | ||
| Hadi Razavinikoo1؛ Hoorieh Soleimanjahi* 1؛ Gholamreza Haqshenas2؛ Taravat Bamdad1؛ Ali Ali Teimoori1؛ Zahra Goodarzi1 | ||
| 1Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran | ||
| 2Department of Microbiology, Faculty of Medicine, Nursing & Health Sciences, School of Biomedical Sciences, Monash University, Melbourne, Victoria, Australia | ||
| چکیده | ||
| Objective(s): The rotavirus nonstructural protein 4 (NSP4) is responsible for the increase in cytoplasmic calcium concentration through a phospholipase C-dependent and phospholipase C-independent pathways in infected cells. It is shown that increasing of intracellular calcium concentration in rotavirus infected cells is associated with the activation of some members of protein kinases family such as calcium/calmodulin-dependent kinase II, which plays a crucial role in replication and pathogenesis of the virus. The aim of this study was to expression bovine rotavirus NSP4 gene in HEK293 cell and evaluation of its biological effect related to activation of calcium/calmodulin-dependent kinase II in cell culture. Materials and Methods: MA104 cells was used as a sensitive cell for propagation of virus and defined as a positive control. The NSP4 gene was amplified and inserted into an expression vector, and introduced as a recombinant plasmid into HEK293T cells. Western blot analysis was performed as a confirmation test for both expression of NSP4 protein and activation of calcium/calmodulin-dependent kinase II. Results:Expression of NSP4 and activated form of calcium/calmodulin-dependent kinase II were demonstrated by western blotting. Conclusion: It was shown that the expression of biologically active full- length NSP4 protein in HEK293T cells may be associated with some biological properties such as calcium calmodulin kinase II activation, which was indicator of rotaviruses replication and pathogenesis | ||
| کلیدواژهها | ||
| Calcium/calmodulin-dependent kinase II؛ Expression؛ Nonstructural protein 4 (NSP4)؛ Rotavirus | ||
| مراجع | ||
|
1. Griffith LC. Calcium/calmodulin-dependent protein kinase II: an unforgettable kinase. J Neurosci 2004; 24:8391-8393.
2. Crawford SE1, Hyser JM, Utama B, Estes MK. Autophagy hijacked through viroporin-activated calcium/calmodulin-dependent kinase kinase-β signaling is required for rotavirus replication. Proc Natl Acad Sci U S A 2012; 109:E3405-3413.
3. Ruiz M, Cohen J, Michelangeli F. Role of Ca 2+ in the replication and pathogenesis of rotavirus and other viral infections. Cell Calcium 2000; 28:137-149.
1. Griffith LC. Calcium/calmodulin-dependent protein kinase II: an unforgettable kinase. J Neurosci 2004; 24:8391-8393.
2. Crawford SE1, Hyser JM, Utama B, Estes MK. Autophagy hijacked through viroporin-activated calcium/calmodulin-dependent kinase kinase-β signaling is required for rotavirus replication. Proc Natl Acad Sci U S A 2012; 109:E3405-3413.
3. Ruiz M, Cohen J, Michelangeli F. Role of Ca 2+ in the replication and pathogenesis of rotavirus and other viral infections. Cell Calcium 2000; 28:137-149.
4. Angel J, Franco MA, Greenberg HB. Rotavirus vaccines: recent developments and future considerations. Nat Rev Microbiol 2007; 5:529-539.
5. Michelangeli F, Ruiz M-C, Del Castillo JR, Ludert JE, Liprandi F. Effect of rotavirus infection on intracellular calcium homeostasis in cultured cells. Virology 1991; 181:520-527.
6. Hyser JM, Collinson-Pautz MR, Utama B, Estes MK. Rotavirus disrupts calcium homeostasis by NSP4 viroporin activity. MBio 2010; 1:e00265-310.
7. Dong Y, Zeng CQ-Y, Ball JM, Estes MK, Morris AP. The rotavirus enterotoxin NSP4 mobilizes intracellular calcium in human intestinal cells by stimulating phospholipase C-mediated inositol 1, 4, 5-trisphosphate production. Proc Natl Acad Sci 1997; 94:3960-3965.
8. Berkova Z, Crawford S, Trugnan G, Yoshimori T, Morris A, Estes M. Rotavirus NSP4 induces a novel vesicular compartment regulated by calcium and associated with viroplasms. J Virol 2006; 80:6061-6071.
9. Barrige M, Bootman M, Roderick H. Calcium signaling: dynamics, homeostasis, and remodeling. Nature 2003; 4:517-529.
10. Giese KP, Fedorov NB, Filipkowski RK, Silva AJ. Autophosphorylation at Thr286 of the α calcium-calmodulin kinase II in LTP and learning. Science 1998; 279:870-873.
11. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: Cold spring harbor laboratory press New York; 1989.
12. Braun AP, Schulman H. The multifunctional calcium/calmodulin-dependent protein kinase: from form to function. Ann Rev Physiol 1995; 57:417-445.
13. Meyer T, Hanson PI, Stryer L, Schulman H. Calmodulin trapping by calcium-calmodulin-dependent protein kinase. Science 1992; 256:1199-1202.
14. Hagbom M, Istrate C, Engblom D, Karlsson T, Rodriguez-Diaz J, Buesa J, et al. Rotavirus stimulates release of serotonin (5-HT) from human enterochromaffin cells and activates brain structures involved in nausea and vomiting. PLoS Pathog 2011; 7:e1002115.
15. Pauly RR, Bilato C, Sollott SJ, Monticone R, Kelly PT, Lakatta EG, et al. Role of calcium/calmodulin-dependent protein kinase II in the regulation of vascular smooth muscle cell migration. Circulation 1995; 91:1107-1115.
16. Brunet J-P, Jourdan N, Cotte-Laffitte J, Linxe C, Géniteau-Legendre M, Servin A, et al. Rotavirus infection induces cytoskeleton disorganization in human intestinal epithelial cells: implication of an increase in intracellular calcium concentration. J Virol 2000; 74:10801-1086.
17. Weclewicz K, Kristensson K, Svensson L. Rotavirus causes selective vimentin reorganization in monkey kidney CV-1 cells. J Gen Virol 1994; 75:3267-3271.
18. Jourdan N, Brunet JP, Sapin C, Blais A, Cotte-Laffitte J, Forestier F, et al. Rotavirus infection reduces sucrase-isomaltase expression in human intestinal epithelial cells by perturbing protein targeting and organization of microvillar cytoskeleton. J Virol 1998; 72:7228-7236.
19. Berridge MJ, Lipp P, Bootman MD. The versatility and universality of calcium signalling. Nat Rev Mol Cell Biol 2000; 1:11-21. 20. Clapham DE. Calcium signaling. Cell 2007; 131:1047-1058.
21. Chattopadhyay S, Basak T, Nayak MK, Bhardwaj G, Mukherjee A, Bhowmick R, et al. Identification of cellular calcium binding protein calmodulin as a regulator of rotavirus A infection during comparative proteomic study. PLoS One 2013; 8:e56655.
22. Teo CS, Chu JJ. Cellular vimentin regulates construction of dengue virus replication complexesthrough interaction with NS4A protein. J Virol 2014; 88:1897-1913.
23. Haolong C, Du N, Hongchao T, Yang Y, Wei Z, Hua Z, et al. Enterovirus 71 VP1 activates calmodulin-dependent protein kinase II and results in the rearrangement of vimentin in human astrocyte cells. PLoS One 2013; 8:e73900.
24. Stefanovic S1, Windsor M, Nagata KI, Inagaki M, Wileman T. Vimentin rearrangement during African swine fever virus infection involves retrograde transport along microtubules and phosphorylation of vimentin by calcium calmodulin kinase II. J Virol 2005; 79:11766-11775. | ||
|
آمار تعداد مشاهده مقاله: 1,973 تعداد دریافت فایل اصل مقاله: 1,591 |
||