Immune reactivity of sera obtained from brucellosis patients and vaccinated-rabbits to a fusion protein from Brucella melitensis | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 6، دوره 18، شماره 4، تیر 2015، صفحه 350-355 اصل مقاله (1.49 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2015.4283 | ||
| نویسندگان | ||
| Jafar Amani* 1؛ Amir Ghasemi2؛ Reza Ranjbar2؛ Mahdi Shabani3؛ Mahdi Zandemami3؛ Reza Golmohammadi2 | ||
| 1Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran | ||
| 2Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran | ||
| 3Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran | ||
| چکیده | ||
| Objective(s): Brucella spp. are facultative intracellular pathogens which can stay alive and multiply in professional and nonprofessional phagocytes. Immunity against Brucella melitensis involves antigen-specific CD4+ and CD8+ T-cells activation and humoral immune responses. Due to negative aspects of live attenuated vaccines, much attention has been focused on finding Brucella-protective antigens to introduce them as potential subunit vaccine candidates. Materials and Methods: A chimeric gene encoding trigger factor (TF), Omp3148-74 and BP2687-111 fragments (TOB) from B. melitensis was successfully cloned, expressed in Escherichia coliBL21-DE3 and purified by Ni-NTA agarose column. Antibodies to recombinant TOB (rTOB) have been investigated in Brucella-infected human sera and a pool serum prepared from B. melitensis-vaccinated rabbits. Results: Our results showed that the immunized rabbit pool serum strongly reacted with rTOB. In addition, antibodies against rTOB were detectable in 76.5% of sera obtained from infected patients. Conclusion: These findings suggest that rTOB may provide a potential immunogenic candidate which could be considered in future vaccine studies. | ||
| کلیدواژهها | ||
| Brucella melitensis؛ ELISA؛ Fusion protein؛ Immune reactivity؛ Vaccine | ||
| مراجع | ||
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