Niapour, Nazila, Mohammadi-Ghalehbin, Behnam, Golmohammadi, Mohammad Ghasem, Amani, Mohammad, Salehi, Hossein, Niapour, Ali. (1393). Efficacy of optimized in vitro predegeneration period on the cell count and purity of canine Schwann cell cultures. , 18(3), 307-311. doi: 10.22038/ijbms.2015.4136
Nazila Niapour; Behnam Mohammadi-Ghalehbin; Mohammad Ghasem Golmohammadi; Mohammad Amani; Hossein Salehi; Ali Niapour. "Efficacy of optimized in vitro predegeneration period on the cell count and purity of canine Schwann cell cultures". , 18, 3, 1393, 307-311. doi: 10.22038/ijbms.2015.4136
Niapour, Nazila, Mohammadi-Ghalehbin, Behnam, Golmohammadi, Mohammad Ghasem, Amani, Mohammad, Salehi, Hossein, Niapour, Ali. (1393). 'Efficacy of optimized in vitro predegeneration period on the cell count and purity of canine Schwann cell cultures', , 18(3), pp. 307-311. doi: 10.22038/ijbms.2015.4136
Niapour, Nazila, Mohammadi-Ghalehbin, Behnam, Golmohammadi, Mohammad Ghasem, Amani, Mohammad, Salehi, Hossein, Niapour, Ali. Efficacy of optimized in vitro predegeneration period on the cell count and purity of canine Schwann cell cultures. , 1393; 18(3): 307-311. doi: 10.22038/ijbms.2015.4136

Efficacy of optimized in vitro predegeneration period on the cell count and purity of canine Schwann cell cultures

Iranian Journal of Basic Medical Sciences
مقاله 14، دوره 18، شماره 3، 2015، صفحه 307-311    اصل مقاله (1.51 M)
شناسه دیجیتال (DOI): 10.22038/ijbms.2015.4136
نویسندگان
Nazila Niapour1؛ Behnam Mohammadi-Ghalehbin2؛ Mohammad Ghasem Golmohammadi1؛ Mohammad Amani3؛ Hossein Salehi4؛ Ali Niapour* 1
1Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
2Department of Microbiology and Parasitology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
3Department of Physiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
4Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده
Objective(s): Predegeneration is a standard technique to obtain mitotically activated and enriched cultures of Schwann cells (SCs). This study, for the first time, evaluated the impact of various duration of predegeneration on cell yield and enrichment of SCs from dog peripheral nerve.
Materials and Methods: Dog sural nerves were subjected to 5, 10, 15 day-long in vitro predegeneration. The total cell yield and the purity of SCs were evaluated in each group on the first and seventh day after plating.
Results: The maximum and minimum numbers of cells were counted in 15 day-long predegene-ration and control groups which underwent no predegeneration. The 10 day-long in vitro predegeneration group with 80±0.5% SCs enrichment had the best purity after plating day and could maintain its purity with elapsing on cultures.
Conclusion: 10 day-long predegeneration results in the higher cell number and the better and prolonged purity of SCs in culture.
کلیدواژه‌ها
Dog؛ Duration؛ Sural nerve؛ Wallerian degeneration؛ Schwann cell
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