Kaeidi, Ayat, Taati, Majid, Hajializadeh, Zahra, Jahandari, Faranak, Rashidipour, Marzieh. (1393). Aqueous extract of Zizyphus jujuba fruit attenuates glucose induced neurotoxicity in an in vitro model of diabetic neuropathy. , 18(3), 301-306. doi: 10.22038/ijbms.2015.4135
Ayat Kaeidi; Majid Taati; Zahra Hajializadeh; Faranak Jahandari; Marzieh Rashidipour. "Aqueous extract of Zizyphus jujuba fruit attenuates glucose induced neurotoxicity in an in vitro model of diabetic neuropathy". , 18, 3, 1393, 301-306. doi: 10.22038/ijbms.2015.4135
Kaeidi, Ayat, Taati, Majid, Hajializadeh, Zahra, Jahandari, Faranak, Rashidipour, Marzieh. (1393). 'Aqueous extract of Zizyphus jujuba fruit attenuates glucose induced neurotoxicity in an in vitro model of diabetic neuropathy', , 18(3), pp. 301-306. doi: 10.22038/ijbms.2015.4135
Kaeidi, Ayat, Taati, Majid, Hajializadeh, Zahra, Jahandari, Faranak, Rashidipour, Marzieh. Aqueous extract of Zizyphus jujuba fruit attenuates glucose induced neurotoxicity in an in vitro model of diabetic neuropathy. , 1393; 18(3): 301-306. doi: 10.22038/ijbms.2015.4135

Aqueous extract of Zizyphus jujuba fruit attenuates glucose induced neurotoxicity in an in vitro model of diabetic neuropathy

Iranian Journal of Basic Medical Sciences
مقاله 13، دوره 18، شماره 3، 2015، صفحه 301-306    اصل مقاله (1.55 M)
شناسه دیجیتال (DOI): 10.22038/ijbms.2015.4135
نویسندگان
Ayat Kaeidi1؛ Majid Taati* 1؛ Zahra Hajializadeh2؛ Faranak Jahandari2؛ Marzieh Rashidipour3
1Department of Physiology, School of Veterinary Medicine, Lorestan University, Khorramabad, Iran
2Kerman Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran
3Young Researchers Club, Khoramabad Brach, Islamic Azad University, Khoramabad, Iran
چکیده
Objective(s):The neuroprotective effect offruit aqueous extract of Ziziphus jujuba Lam on glucose-induced neurotoxicity in PC12 cells as an appropriate in vitro model of diabetic neuropathy was investigated.
Materials and Methods: Cell viability was determined by the MTT assay. Cellular reactive oxygen species (ROS) generation was measured by DCFH-DA analysis. Cleaved caspase-3, a biochemical parameter of cellular apoptosis, was measured by western blot analysis.
Results: Our data showed that a 4-fold elevation in glucose levels within the medium significantly reduced cell viability, increased intracellular ROS and caspase-3 activation in PC12 cells after 24 hr. Incubation of the high glucose medium cells with 300-μg/ml Z. jujuba fruit (ZJF) extract decreased the high glucose-induced cell toxicity and prevented caspase-3 activation and excited ROS generation.
Conclusion: Thus, we concluded that the aqueous extract of Z. jujuba protects against hyperglycaemia-induced cellular toxicity. This could be associated with the prevention of ROS generation and neural apoptosis. Moreover, the results suggest that the ZJF has a therapeutic potential to attenuate diabetes complications such as neuropathy.
کلیدواژه‌ها
Apoptosis؛ Diabetes؛ Neuropathy؛ Ziziphus Jujube
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