Navaei-Nigjeh, Mona, Asadi, Hamidreza, Baeeri, Maryam, Pedram, Sahar, Rezvanfar, Mohammad Amin, Mohammadirad, Azadeh, Abdollahi, Mohammad. (1393). In vitro protection of human lymphocytes from toxic effects of chlorpyrifos by selenium-enriched medicines. , 18(3), 284-292. doi: 10.22038/ijbms.2015.4133
Mona Navaei-Nigjeh; Hamidreza Asadi; Maryam Baeeri; Sahar Pedram; Mohammad Amin Rezvanfar; Azadeh Mohammadirad; Mohammad Abdollahi. "In vitro protection of human lymphocytes from toxic effects of chlorpyrifos by selenium-enriched medicines". , 18, 3, 1393, 284-292. doi: 10.22038/ijbms.2015.4133
Navaei-Nigjeh, Mona, Asadi, Hamidreza, Baeeri, Maryam, Pedram, Sahar, Rezvanfar, Mohammad Amin, Mohammadirad, Azadeh, Abdollahi, Mohammad. (1393). 'In vitro protection of human lymphocytes from toxic effects of chlorpyrifos by selenium-enriched medicines', , 18(3), pp. 284-292. doi: 10.22038/ijbms.2015.4133
Navaei-Nigjeh, Mona, Asadi, Hamidreza, Baeeri, Maryam, Pedram, Sahar, Rezvanfar, Mohammad Amin, Mohammadirad, Azadeh, Abdollahi, Mohammad. In vitro protection of human lymphocytes from toxic effects of chlorpyrifos by selenium-enriched medicines. , 1393; 18(3): 284-292. doi: 10.22038/ijbms.2015.4133

In vitro protection of human lymphocytes from toxic effects of chlorpyrifos by selenium-enriched medicines

Iranian Journal of Basic Medical Sciences
مقاله 11، دوره 18، شماره 3، 2015، صفحه 284-292    اصل مقاله (1.72 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2015.4133
نویسندگان
Mona Navaei-Nigjeh1؛ Hamidreza Asadi2؛ Maryam Baeeri3؛ Sahar Pedram2؛ Mohammad Amin Rezvanfar3؛ Azadeh Mohammadirad3؛ Mohammad Abdollahi* 4
1Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
2Toxicology and Poisoning Research Center and Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
3Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran
4Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran. Toxicology and Poisoning Research Center and Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
چکیده
Objective(s):  Chlorpyrifos (CP) is a broad-spectrum organophosphorus pesticide used extensively in agricultural and domestic pest control, accounting for 50% of the global insecticidal use. In the present study, protective effects of two selenium-enriched strong antioxidative medicines IMOD and Angipars were examined in human lymphocytes treated with CP in vitro.
Materials and Methods: Isolated lymphocytes were exposed to 12 µg/ml CP either alone or in combination with effective doses (ED50) of IMOD (0.2 µg/ml) and Angipars (1 µg/ml). After 3 days incubation, the viability and oxidative stress markers including cellular lipid peroxidation (LPO), myeloperoxidase (MPO), total thiol molecules (TTM), and total antioxidant power (TAP) were evaluated. Also, the levels of tumor necrosis factor-α (TNF-α), as inflammatory index along with acetylcholinesterase (AChE) activity and cell apoptosis were assessed by flow cytometry.
Results: Results indicated that effective doses of IMOD and Angipars reduced CP-exposed lymphocyte mortality rate along with oxidative stress. Both agents restored CP-induced elevation of TNF-α and protected the lymphocytes from CP-induced apoptosis and necrosis.
Conclusion: Overall, results confirm that IMOD and Angipars reduce the toxic effects associated with CP through free radical scavenging and protection from apoptosis and necrosis.
کلیدواژه‌ها
Angipars؛ Chlorpyrifos؛ Human lymphocytes؛ IMOD؛ Organophosphorus Oxidative stress
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