Azizi, Saeed, Hamidi Alamdari, Daryoush, Amini, Keyvan, Raisi, Abbas, Azimzadeh, Mohammad. (1393). Alpha-lipoic acid loaded in chitosan conduit enhances sciatic nerve regeneration in rat. , 18(3), 228-233. doi: 10.22038/ijbms.2015.4120
Saeed Azizi; Daryoush Hamidi Alamdari; Keyvan Amini; Abbas Raisi; Mohammad Azimzadeh. "Alpha-lipoic acid loaded in chitosan conduit enhances sciatic nerve regeneration in rat". , 18, 3, 1393, 228-233. doi: 10.22038/ijbms.2015.4120
Azizi, Saeed, Hamidi Alamdari, Daryoush, Amini, Keyvan, Raisi, Abbas, Azimzadeh, Mohammad. (1393). 'Alpha-lipoic acid loaded in chitosan conduit enhances sciatic nerve regeneration in rat', , 18(3), pp. 228-233. doi: 10.22038/ijbms.2015.4120
Azizi, Saeed, Hamidi Alamdari, Daryoush, Amini, Keyvan, Raisi, Abbas, Azimzadeh, Mohammad. Alpha-lipoic acid loaded in chitosan conduit enhances sciatic nerve regeneration in rat. , 1393; 18(3): 228-233. doi: 10.22038/ijbms.2015.4120

Alpha-lipoic acid loaded in chitosan conduit enhances sciatic nerve regeneration in rat

Iranian Journal of Basic Medical Sciences
مقاله 3، دوره 18، شماره 3، 2015، صفحه 228-233    اصل مقاله (1.53 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2015.4120
نویسندگان
Saeed Azizi* 1؛ Daryoush Hamidi Alamdari2؛ Keyvan Amini3؛ Abbas Raisi4؛ Mohammad Azimzadeh1
1Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
2Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Stem Cell & Regenerative Medicine Research Group, Department of Clinical Biochemistry, Mashhad University of Medical Sciences, Mashhad, Iran
3Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
4Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khoramabad, Iran
چکیده
Objective(s): To investigate the effect of topical administration of alpha-lipoic acid into chitosan conduit on peripheral nerve regeneration using a rat sciatic nerve transection model.
Materials and Methods: Forty five Wistar rats were divided into three experimental groups randomly. A 10-mm gap of sciatic nerve was bridged with a chitosan conduit following surgical preparation and anesthesia. In treatment group, the conduit was filled with 30 µl alpha-lipoic acid (10 mg/kg/bw).It was filled with 30 µl phosphate buffered saline solution in control group. In Sham group sciatic nerve was just exposed.
Results: The recovery of nerve function was faster in treatment group than in control, at 4 and 8 weeks after surgery (P-value<0.05). Conduction velocity was better in treatment group than in control group at 4 and 12 weeks (P-value<0.05). Recovery index was higher in treatment group than the control group, 8 weeks after surgery (P-value <0.05). Greater nerve fiber diameter, axon diameter, and myelin sheath thickness were observed in treatment group compared to control group at 8 and 12 weeks after surgery (P-value<0.05). The immunoreactivity of regenerated axons and myelin sheath in treatment group were far more similar to sham group.
Conclusion: Alpha-lipoic acid when loaded in a chitosan conduit could improve transected sciatic nerve regeneration in rat.
کلیدواژه‌ها
Alpha-lipoic acid؛ Chitosan conduit؛ Rat؛ Sciatic nerve regeneration
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