In vitro differentiation of rat mesenchymal stem cells to hepatocyte lineage | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 14، دوره 18، شماره 1، فروردین 2015، صفحه 89-97 اصل مقاله (1.76 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2015.3894 | ||
| نویسندگان | ||
| Samaneh Solati Sarvandi1؛ Mohammad Taghi Joghataei2؛ Kazem Parivar3؛ Maryam Khosravi1؛ Arash Sarveazad4؛ Nima Sanadgol* 5 | ||
| 1Department of Biology, Faculty of Biological Sciences, North Branch of Islamic Azad University, Tehran, Iran | ||
| 2Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran | ||
| 3Department of Biology, Faculty of Basic Science, Sciences and Researches branch of Islamic Azad University, Tehran, Iran | ||
| 4Department of Anatomy, School of medicine, Iran University of Medical Sciences, Tehran, Iran | ||
| 5Department of Biology, Faculty of Science, University of Zabol, Zabol, Iran. Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran | ||
| چکیده | ||
| Objective(s): Mesenchyme is a type of undifferentiated loose connective tissue that is derived mostly from mesoderm. Recently, mesenchymal stem cells (MSCs), as adult stem cells (ASCs) able to divide into a variety of different cells, are of utmost importance for stem cell research. In this research, ability of the liver extract to induce differentiation of rat derived omentum tissue mesenchymal stem cells (rOT-MSCs) into hepatocyte cells (HCs) was investigated. Materials and Methods: After isolation and confirmation of rOT-MSCs they were co-cultured with liver extract and hepatogenic differentiation was monitored. Expressions of mesenchymal stem cell markers were also analyzed via flow cytometry. Moreover, expressions of octamer-binding transcription factor-4 (Oct-4), Wilm's tumor suppressor gene-1 (WT-1), albumin (ALB), alpha fetoprotein (AFP), cytokeratin-18 (CK-18), and mRNAs were analyzed using RT-PCR on days 16, 18 and 21. ALB production was analyzed by immunocytochemistry and western blot. Furthermore, glycogen and urea production were determined via periodic acid-Schiff (PAS) staining and colorimetric assays respectively. Results:The phenotypic characterization revealed the positive expressions of CD90, CD44 and negative expression of CD45 inrOT-MSCs. These cells also expressed mRNA of Oct-4 and WT-1 as markers of omentum tissue. Differentiated rOT-MSCs in presence of 6 µg/ml liver extract expressed ALB, AFP, CK-18, glycogen and urea as specific markers of HCs. Conclusion: These observations suggest that liver extract is potentially able to induce differentiation of MSCs into hepatocyte lineage and can be considered an available source for imposing tissue healing on the damaged liver. | ||
| کلیدواژهها | ||
| Hepatocyte؛ Liver extract؛ Mesenchymal stem cells؛ Omentum tissue | ||
| مراجع | ||
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