Human Wharton’s jelly-derived mesenchymal stem cells express oocyte developmental genes during co-culture with placental cells | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 5، دوره 18، شماره 1، فروردین 2015، صفحه 22-29 اصل مقاله (1.69 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2015.3883 | ||
| نویسندگان | ||
| Hamid Reza Asgari1؛ Mohammad Akbari1؛ Mehdi Abbasi* 1؛ Jafar Ai2؛ Morteza Korouji3؛ Fereshte Aliakbari1؛ Kehinde Adebayo Babatunde1؛ Fereydoon Sargolzaei Aval4؛ Mohammad Taghi Joghataei3 | ||
| 1Department of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran | ||
| 2Department of Tissue engineering, School of Advanced Technologies, Tehran University of Medical Sciences, Tehran, Iran | ||
| 3Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran | ||
| 4Department of Anatomy, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran | ||
| چکیده | ||
| Objective(s): The present day challenge is how to obtain germ cells from stem cells to treat patients with cancer and infertility. Much more efforts have been made to develop a procedure for attaining germ cells in vitro. Recently, human umbilical cord-derived mesenchymal stem cells (HUMSCs) have been introduced with higher efficacy for differentiation. In this work, we tried to explore the efficacy of HUMSCs and some effective products of placental cells such as transforming growth factors. This study is aimed to optimize a co-culture condition for HUMSCs with placental cells to obtain primordial germ cells (PGCs) and reach into oocyte-like cells in vitro. Materials and Methods: In this experimental study, HUMSCs and placental cells were co-cultured for 14 days without any external inducer in vitro. Then HUMSCs were assessed for expression of PGC markers; Octamer-binding transcription factor 4(OCT4),Tyrosine-protein kinase Kit (CKIT), Stage specific embryonic antigen 4 (SSEA4) , DEAD (Asp-Glu-Ala-Asp) box polypeptide 4( DDX4) and oocyte specific markers; Growth differentiation factor-9( GDF9), Zona pellucida glycoprotein 3(ZP3). The pertinent markers were assessed by immunocytochemistry and Q-PCR. Results: Co-cultured HUMSCs with placental cells (including amniotic and chorionic cells) presented Oct4 and DDX4, primordial germ cells specific markers significantly, but increment in expression of oocyte-like cell specific markers, GDF9 and ZP3 did not reach to statistically significant threshold. Conclusion: Placental cell supplementsTransforming growth factor (TGF α, β) and basic fibroblast growth factor (bFGF) in a co-culture model can provide proper environment for induction of HUMSCs into PGCs and expression of oocyte-like markers. | ||
| کلیدواژهها | ||
| Co-culture؛ Human umbilical cord؛ Mesenchymal stem cells؛ Oocyte-like cell؛ Placental cells | ||
| مراجع | ||
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