سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Havakhah, Shahrzad, Sadeghnia, Hamid R, Hajzadeh, Mosa-Al-Reza, Mohammadian Roshan, Nama, Shafiee, Somayeh, Hosseinzadeh, Hossein, Mohareri, Narges, Khajavi Rad, Abolfazl. (1393). Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage. , 17(12), 986-992. doi: 10.22038/ijbms.2015.3856
Shahrzad Havakhah; Hamid R Sadeghnia; Mosa-Al-Reza Hajzadeh; Nama Mohammadian Roshan; Somayeh Shafiee; Hossein Hosseinzadeh; Narges Mohareri; Abolfazl Khajavi Rad. "Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage". , 17, 12, 1393, 986-992. doi: 10.22038/ijbms.2015.3856
Havakhah, Shahrzad, Sadeghnia, Hamid R, Hajzadeh, Mosa-Al-Reza, Mohammadian Roshan, Nama, Shafiee, Somayeh, Hosseinzadeh, Hossein, Mohareri, Narges, Khajavi Rad, Abolfazl. (1393). 'Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage', , 17(12), pp. 986-992. doi: 10.22038/ijbms.2015.3856
Havakhah, Shahrzad, Sadeghnia, Hamid R, Hajzadeh, Mosa-Al-Reza, Mohammadian Roshan, Nama, Shafiee, Somayeh, Hosseinzadeh, Hossein, Mohareri, Narges, Khajavi Rad, Abolfazl. Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage. , 1393; 17(12): 986-992. doi: 10.22038/ijbms.2015.3856

Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage

Iranian Journal of Basic Medical Sciences
مقاله 8، دوره 17، شماره 12، اسفند 2014، صفحه 986-992    اصل مقاله (1.69 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2015.3856
نویسندگان
Shahrzad Havakhah1؛ Hamid R Sadeghnia2؛ Mosa-Al-Reza Hajzadeh3؛ Nama Mohammadian Roshan4؛ Somayeh Shafiee1؛ Hossein Hosseinzadeh5؛ Narges Mohareri6؛ Abolfazl Khajavi Rad* 7
1Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3Neurocognitive Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4Department of Pathology, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
5Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
6Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
7Neuroinflammation Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s):There are a few previously reported studies about the effect of Nigella sativa oil on renal ischemia-reperfusion injury (IRI). The aim of the present study was to test the hypothesis whether pre- or post-treatment with N. sativa hydroalcoholic extract (NSE) would reduce tissue injury and oxidative damages in a clinically relevant rat model of renal IRI.   
Materials and Methods: IRI was induced by clamping of bilateral renal arteries for 40 min fallowed by reperfusion for 180 min. NSE was prepared in a Soxhlet extractor and administrated with doses of 150 mg/kg or 300 mg/kg at 1 hr before ischemia induction (P-150 and 300) or at the beginning of reperfusion phase (T-150 and 300), via jugular catheter intravenously. The kidneys were then removed and subjected to biochemical analysis, comet assay or histopathological examination.
Results: The kidneys of untreated IRI rats had a higher histopathological score (P<0.001), while in P-150, as well as T-150 and T-300 groups tubular lesions significantly decreased (P<0.001). Pre- and post-treatment with NSE also resulted in a significant decrease in malondialdehyde (MDA) level (P<0.001) and DNA damage (P<0.001) that were increased by renal I/R injury. NSE treatment also significantly restore (P<0.01) the decrease in renal thiol content caused by IRI.
Conclusion: The present study shows N. sativa extract has marked protective action against renal IRI, which may be partly due to its antioxidant effects.
کلیدواژه‌ها
Comet assay؛ Ischemia-reperfusion injury (IRI)؛ Kidney؛ Nigella Sativa؛ Oxidative stress
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