Evaluating the effects of galbanic acid on hydrogen peroxide-induced oxidative DNA damage in human lymphocytes | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 5، دوره 4، شماره 5، آذر و دی 2014، صفحه 337-342 اصل مقاله (448.24 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2014.3040 | ||
| نویسندگان | ||
| Kobra Shirani1؛ Javad Behravan2؛ Fatemeh Mosaffa2؛ Mehrdad Iranshahi3؛ Babak Mehmankhah1؛ Kamal Razavi-Azarkhiavi1؛ Gholamreza Karimi* 4 | ||
| 1Department of Pharmacodynamy and Toxicology, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, I. R. Iran | ||
| 2Biotechnology Research Center and Pharmacy School, Mashhad University of Medical Sciences, Mashhad, I. R. Iran | ||
| 3Department of Pharmacognosy and Biotechnology, Faculty of Pharmacy, Mashhad University of Medical Sciences, I. R. Iran | ||
| 4Medical Toxicology Research Center and Pharmacy School, Mashhad University of Medical Sciences, Mashhad, I. R. Iran | ||
| چکیده | ||
| Objective: Ferula szowitsiana has been widely used for medicinal purposes around the world. The anti-oxidant effect of F.szowitsiana had been proved. The current study aims to determine the protective effects of galbanic acid, a sesquiterpene coumarin from F. szowitsiana, against hydrogen peroxide (H2O2) - induced oxidative DNA damage in human lymphocytes. Materials and Methods: Human lymphocytes were incubated with H2O2 (0, 25, 50, 100, and 200 µM), galbanic acid (200 and 400 µM) and a combination of galbanic acid (200 and 400 µM) and H2O2 (25 µM) at 4 C for 30 minutes. Solvents of galbanic acid without H2O2 were used as negative controls. Results: The findings of this study demonstrated that H2O2 exposure leads to a significant concentration-dependent increase in DNA damage. Galbanic acid did not cause DNA damage compared with the control cells. Data showed that galbanic acid does not have a protective effect against H2O2-induced oxidative DNA damage in human lymphocytes. Conclusion: According to the results, it is concluded that the capability of F. szowitsiana in reducing reactive oxygen species and the anti-inflammatory property of its methanolic extract may be due to its other ingredients. | ||
| کلیدواژهها | ||
| Comet assay؛ DNA damage؛ Galbanic acid؛ Ferula szowitsiana | ||
| مراجع | ||
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