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Yesildag, Sahin, Yılmaz Akyuz, Elvan, Taskin, Eylem. (1405). Investigating the protective effect of zinc supplementation on streptozotocin-induced pancreatic beta-cell damage via the HMGB1/TLR2/MAPK pathway. , (), -. doi: 10.22038/ijbms.2026.92275.19914
Sahin Yesildag; Elvan Yılmaz Akyuz; Eylem Taskin. "Investigating the protective effect of zinc supplementation on streptozotocin-induced pancreatic beta-cell damage via the HMGB1/TLR2/MAPK pathway". , , , 1405, -. doi: 10.22038/ijbms.2026.92275.19914
Yesildag, Sahin, Yılmaz Akyuz, Elvan, Taskin, Eylem. (1405). 'Investigating the protective effect of zinc supplementation on streptozotocin-induced pancreatic beta-cell damage via the HMGB1/TLR2/MAPK pathway', , (), pp. -. doi: 10.22038/ijbms.2026.92275.19914
Yesildag, Sahin, Yılmaz Akyuz, Elvan, Taskin, Eylem. Investigating the protective effect of zinc supplementation on streptozotocin-induced pancreatic beta-cell damage via the HMGB1/TLR2/MAPK pathway. , 1405; (): -. doi: 10.22038/ijbms.2026.92275.19914

Investigating the protective effect of zinc supplementation on streptozotocin-induced pancreatic beta-cell damage via the HMGB1/TLR2/MAPK pathway

Iranian Journal of Basic Medical Sciences
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 27 اردیبهشت 1405    اصل مقاله (544.13 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2026.92275.19914
نویسندگان
Sahin Yesildag1؛ Elvan Yılmaz Akyuz2؛ Eylem Taskin* 3، 4
1Faculty of Health Sciences, Department of Nutrition and Dietetics, Demiroglu Bilim University, Istanbul, Türkiye
2Faculty of Hamidiye Health Sciences, Department of Nutrition and Dietetics, University of Health Sciences, Istanbul, Türkiye
3Faculty of Medicine, Department of Physiology, Adiyaman University, Adiyaman, Türkiye
4Health Science Institute, Translational Medicine, Cukurova University, Adana, Türkiye
چکیده
Objective(s): This study investigated the protective effect of zinc supplementation on insulin release via modulation of the High Mobility Group Box 1 (HMGB1)/Toll-like receptor 2 (TLR2)/mitogen-activated protein kinase (MAPK) signaling pathway in streptozotocin (STZ)-induced pancreatic β-cell injury, a commonly used model of type 1 diabetes.
Materials and Methos: Human pancreatic β-cells (1.1B4) were divided into five groups: Control, Diabetes, Diabetes + Zinc, Diabetes + Box A, and Diabetes + Zinc + Box A. Diabetes was induced with STZ (20 mM, four hours, type-1), followed by treatment with zinc (30 μM) and/or Box A (100 ng/l). Cellular oxidative stress, apoptosis, mitochondrial membrane potential (MMP), cell viability, and insulin secretion were measured. Protein expressions of HMGB1, TLR2/4, extracellular signal-regulated kinases 1/2 (ERK1/2), and c-Jun N-terminal kinase (JNK) were analyzed using western blotting.
Results: Zinc supplementation restored MMP and total antioxidant status (TAS), reduced apoptotic cell death, and enhanced TLR2 and ERK1/2 expression suppressed by STZ. Combined zinc and Box A treatment markedly increased insulin secretion under both hypo- and hyperglycemic conditions.
Conclusion: Zinc exerts protective effects against STZ-induced β-cell damage, likely through modulation of the HMGB1/TLR2/MAPK axis. These findings highlight the therapeutic potential of zinc supplementation in protecting pancreatic β-cell function in diabetes mellitus.
کلیدواژه‌ها
Apoptosis؛ Diabetes mellitus؛ HMGB1 protein؛ Toll-like receptor 2؛ Zinc
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