Genetic and Epigenetic landscape of Germline Stem Cells | ||
| Journal of Pediatric Perspectives | ||
| مقاله 14، دوره 2، شماره 2.3، مرداد 2014، صفحه 14-14 اصل مقاله (104.95 K) | ||
| شناسه دیجیتال (DOI): 10.22038/ijp.2014.2663 | ||
| نویسندگان | ||
| Moein Farshchian1؛ Mahtab Dastpak2؛ Maryam M. Matin2؛ Dirk Geerts3؛ Ahmad Reza Bahrami* 1 | ||
| 1Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| 2Cell and Molecular Biotechnology Research Group, Institute of Biotechnology Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| 3Department of Pediatric Oncology / Hematology, Erasmus University Medical Center, Rotterdam, The Netherlands. | ||
| چکیده | ||
| Elucidating the critical epigenetics events involved in differentiation and reprogramming of cells to primordial germ cells (PGCs) is among the interesting issues in stem cell research. Here, I will talk about critical transcription factors and global hypomethylation in development of germ cells. Evidence strongly suggests that the earliest PGCs emerging in the E7.25 mouse embryo epiblast have a highly methylated genome, and high level of H3K9me2 in chromatin but during development, genome demethylated and patterns of histone codes changes dramatically. We designed a polycistroniclentiviral vector and overexpressed Stella, Oct4 and Nanos2 simultaneously in transduced cells; Increasing level of Prdm14, Nanog and decreasing of G9a expression is an interesting finding which might be considered as a primary step of reprogramming toward germline progenitor cells, here we propose decreasing H3K9me2 level as a consequence of G9a down regulation is a critical step which facilitated transition to different stemness state through creating a new epigenetic memory for the early germ cells. Keywords: Epigenetic, hypomethylation, Germ line Stem Cells, polycistroniclentiviral vector. | ||
| کلیدواژهها | ||
| Oral Presentation | ||
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