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Mottaghian, Sadaf, Khalilarya, Ayshin, Malekinejad, Hassan. (1404). Dexamethasone-induced hepatic biochemical and pathological damages are attenuated by Cynodon dactylon extract containing hexadecanoic acid via reduction of glucose uptake: In vitro and in vivo studies. , 16(2), 173-191. doi: 10.22038/ajp.2025.26623
Sadaf Mottaghian; Ayshin Khalilarya; Hassan Malekinejad. "Dexamethasone-induced hepatic biochemical and pathological damages are attenuated by Cynodon dactylon extract containing hexadecanoic acid via reduction of glucose uptake: In vitro and in vivo studies". , 16, 2, 1404, 173-191. doi: 10.22038/ajp.2025.26623
Mottaghian, Sadaf, Khalilarya, Ayshin, Malekinejad, Hassan. (1404). 'Dexamethasone-induced hepatic biochemical and pathological damages are attenuated by Cynodon dactylon extract containing hexadecanoic acid via reduction of glucose uptake: In vitro and in vivo studies', , 16(2), pp. 173-191. doi: 10.22038/ajp.2025.26623
Mottaghian, Sadaf, Khalilarya, Ayshin, Malekinejad, Hassan. Dexamethasone-induced hepatic biochemical and pathological damages are attenuated by Cynodon dactylon extract containing hexadecanoic acid via reduction of glucose uptake: In vitro and in vivo studies. , 1404; 16(2): 173-191. doi: 10.22038/ajp.2025.26623

Dexamethasone-induced hepatic biochemical and pathological damages are attenuated by Cynodon dactylon extract containing hexadecanoic acid via reduction of glucose uptake: In vitro and in vivo studies

Avicenna Journal of Phytomedicine
دوره 16، شماره 2، خرداد و تیر 2026، صفحه 173-191    اصل مقاله (1.54 M)
نوع مقاله: Original Research Article
شناسه دیجیتال (DOI): 10.22038/ajp.2025.26623
نویسندگان
Sadaf Mottaghian1؛ Ayshin Khalilarya2؛ Hassan Malekinejad* 3
1Department of Pharmacology and Toxicology, School of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran
2Experimental & Applied Pharmaceutical Sciences Research Center, Urmia University of Medical Sciences, Urmia, Iran
3a Experimental & Applied Pharmaceutical Sciences Research Center, Urmia University of Medical Sciences, Urmia, Iran; b Department of Pharmacology and Toxicology, School of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran
چکیده
Objective: The protective effects of ethanolic extract of Cynodon dactylon (CD) on dexamethasone (DEX)-induced hepatic injuries were investigated.
Materials and Methods: Following GC-MS phytochemical analyses of the extract, in vivo studies were conducted in male rats which were grouped as: control, dexamethasone (8mg/kg, intraperitoneally), DEX+CD (100, 200 and 400 mg/kg, orally), CD 400 and DEX+metformin (MET, 300mg/kg, orally) for 15 days. The in vitro studies were performed in HepG2 cells that grouped as: Control, DEX (1 µM), DEX+CD (0.1, 0.5 and 1 mg/ml), CD 0.5 mg/ml, and DEX+MET (500 µM). Cell viability and animal weight changes were recorded. Following the study period, biochemical and histopathological analyses were conducted on hepatic tissue and HepG2 cells.
Results: Findings revealed that CD extract does have concentration-dependent free radical-scavenging activity, and it possesses phenols and flavonoids contents. The most abundant compound of the CD extract was n-hexadecanoic acid. The DEX-induced hepatic functional enzymes and lactate dehydrogenase levels were significantly (p<0.05) reduced in both models. Moreover, the DEX-induced oxidative stress was improved by CD extract (45%). The DEX-elevated blood glucose (20%) and triglycerides (60%) were reduced in CD-treated animals. The DEX-generated hepatic macrovesicles and lipid droplets in HepG2 cells were reduced.
Conclusion: These results suggest that CD extract could be an effective compound for protection from DEX-induced hepatic injuries.
کلیدواژه‌ها
Dexamethasone؛ Hepatic injuries؛ Hypolipidemic effect؛ Glucose uptake
آمار
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