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Wang, Guifa, Zhang, Nan, Zhang, Xiaochen, Guo, Zihan, Liu, Man, Xue, Meilan, Liang, Hui. (1404). Evaluating the effect of fucoidan-alginate combined dressing on wound healing in rats with full-thickness skin removed. , 28(11), 1477-1485. doi: 10.22038/ijbms.2025.86338.18650
Guifa Wang; Nan Zhang; Xiaochen Zhang; Zihan Guo; Man Liu; Meilan Xue; Hui Liang. "Evaluating the effect of fucoidan-alginate combined dressing on wound healing in rats with full-thickness skin removed". , 28, 11, 1404, 1477-1485. doi: 10.22038/ijbms.2025.86338.18650
Wang, Guifa, Zhang, Nan, Zhang, Xiaochen, Guo, Zihan, Liu, Man, Xue, Meilan, Liang, Hui. (1404). 'Evaluating the effect of fucoidan-alginate combined dressing on wound healing in rats with full-thickness skin removed', , 28(11), pp. 1477-1485. doi: 10.22038/ijbms.2025.86338.18650
Wang, Guifa, Zhang, Nan, Zhang, Xiaochen, Guo, Zihan, Liu, Man, Xue, Meilan, Liang, Hui. Evaluating the effect of fucoidan-alginate combined dressing on wound healing in rats with full-thickness skin removed. , 1404; 28(11): 1477-1485. doi: 10.22038/ijbms.2025.86338.18650

Evaluating the effect of fucoidan-alginate combined dressing on wound healing in rats with full-thickness skin removed

Iranian Journal of Basic Medical Sciences
مقاله 3، دوره 28، شماره 11، بهمن 2025، صفحه 1477-1485    اصل مقاله (532.74 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2025.86338.18650
نویسندگان
Guifa Wang1؛ Nan Zhang2؛ Xiaochen Zhang3؛ Zihan Guo4؛ Man Liu5، 6؛ Meilan Xue* 1؛ Hui Liang* 5
1Department of Biochemistry and Molecular Biology, School of Basic Medicine, Qingdao University, 308 Ningxia Road, Qingdao, 266071, China
22Department of Nutrition and Food Hygiene, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, 266071, China
3Adelaide 128 Waymouth St, Y Suites on Waymouth Adelaide SA 5000 Australia
4Department of Clinical Nutrition, The Second People’s Hospital of Lianyungang, Lianyungang, 222000, China
5Department of Nutrition and Food Hygiene, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, 266071, China
6School of Nursing, Qingdao University, 308 Ningxia Road, Qingdao, 266071, China
چکیده
Objective(s): This study aims to investigate the effects of fucoidan-alginate combined dressings on wound healing in rats with full-thickness skin defects and to explore the underlying mechanisms. 
Materials and Methods: Male SD rats were divided into three groups (n=15): Control, 2% fucoidan, and 5% fucoidan. Full-thickness skin wounds were created on each rat. Fucoidan-alginate dressings were prepared by applying 20 mg/ml and 50 mg/ml fucoidan solutions to alginate dressings (2×2 cm), resulting in 2% and 5% (w/v) fucoidan-alginate combined dressings, respectively. The control group utilized alginate dressings. Wound healing was assessed through various methods, including wound area measurement, histopathological analysis, white blood cell counts, ELISA for TNF-α and IL-1β, Masson’s trichrome staining for collagen, immunohistochemistry for TGF-β1, and western blotting for TGF-β1 and Smad-related proteins.
Results: The results revealed that wound healing was significantly more effective in rats treated with 5% fucoidan-alginate combined dressings. Compared to the control group (P<0.01) and the 2% FUC group (P<0.05), the 5% FUC group exhibited reduced inflammatory cell infiltration and lower levels of TNF-α and IL-1β. Moreover, in comparison to the control group, the 5% FUC group demonstrated a significant up-regulation in the mean density of TGF-β1 (P<0.01) and significantly elevated protein expression levels of Col I, α-SMA, and p-Smad2/3 (P<0.01). Additionally, a notable amount of collagen production was observed.
Conclusion: The findings suggested that fucoidan-alginate dressings promote wound healing, reduce inflammation, and enhance collagen synthesis in rats, likely via the TGF-β1/Smad signaling pathway.
کلیدواژه‌ها
Alginate؛ Combined Dressing Fucoidan؛ Natural product؛ Wound healing
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