سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Kaboodkhani, Reza, Zandifar, Zohreh, Owji, Seyed Hossein, Koohi-Hosseinabadi, Omid, Owji, Seyed Mohammad, Yousefi, Sarah, Mohammadi Oshnari, Abbas, Taheri, Salman, Mohammadi, Ali Asghar, Asmarian, Naeimeh Sadat. (1404). The Effect of the Chitosan on Bleeding Control and Healing of Dog Buccal Mucosal Wound: An in Vivo Experimental Study. , 37(4), 187-196. doi: 10.22038/ijorl.2025.80060.3690
Reza Kaboodkhani; Zohreh Zandifar; Seyed Hossein Owji; Omid Koohi-Hosseinabadi; Seyed Mohammad Owji; Sarah Yousefi; Abbas Mohammadi Oshnari; Salman Taheri; Ali Asghar Mohammadi; Naeimeh Sadat Asmarian. "The Effect of the Chitosan on Bleeding Control and Healing of Dog Buccal Mucosal Wound: An in Vivo Experimental Study". , 37, 4, 1404, 187-196. doi: 10.22038/ijorl.2025.80060.3690
Kaboodkhani, Reza, Zandifar, Zohreh, Owji, Seyed Hossein, Koohi-Hosseinabadi, Omid, Owji, Seyed Mohammad, Yousefi, Sarah, Mohammadi Oshnari, Abbas, Taheri, Salman, Mohammadi, Ali Asghar, Asmarian, Naeimeh Sadat. (1404). 'The Effect of the Chitosan on Bleeding Control and Healing of Dog Buccal Mucosal Wound: An in Vivo Experimental Study', , 37(4), pp. 187-196. doi: 10.22038/ijorl.2025.80060.3690
Kaboodkhani, Reza, Zandifar, Zohreh, Owji, Seyed Hossein, Koohi-Hosseinabadi, Omid, Owji, Seyed Mohammad, Yousefi, Sarah, Mohammadi Oshnari, Abbas, Taheri, Salman, Mohammadi, Ali Asghar, Asmarian, Naeimeh Sadat. The Effect of the Chitosan on Bleeding Control and Healing of Dog Buccal Mucosal Wound: An in Vivo Experimental Study. , 1404; 37(4): 187-196. doi: 10.22038/ijorl.2025.80060.3690

The Effect of the Chitosan on Bleeding Control and Healing of Dog Buccal Mucosal Wound: An in Vivo Experimental Study

Iranian Journal of Otorhinolaryngology
دوره 37، شماره 4، مهر و آبان 2025، صفحه 187-196    اصل مقاله (635.19 K)
نوع مقاله: Original
شناسه دیجیتال (DOI): 10.22038/ijorl.2025.80060.3690
نویسندگان
Reza Kaboodkhani1؛ Zohreh Zandifar2؛ Seyed Hossein Owji* 3؛ Omid Koohi-Hosseinabadi4؛ Seyed Mohammad Owji5؛ Sarah Yousefi6؛ Abbas Mohammadi Oshnari6؛ Salman Taheri6؛ Ali Asghar Mohammadi6؛ Naeimeh Sadat Asmarian7
1Otolaryngology Research Center, Department of Otolaryngology, Shiraz University of Medical Sciences, Shiraz, Iran.
2Otorhinolaryngologist and Head and Neck Surgeon, Private Practice, Shiraz, Iran.
3Otolaryngology Research Center, Department of Otolaryngology, Shiraz University of Medical Sciences, Shiraz, Iran. Otorhinolaryngology Research Center, Otorhinolaryngology Head and Neck Surgery Department, Imam Khomeni Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran.
4Laparoscopy Research Center, Shiraz University of medical Science, Shiraz, Iran.
5Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran .
6Chemistry and Chemical Engineering Research Center of Iran (CCERCI), P.O. Box 14335-186, Tehran, Iran.
7Anesthesiology and Critical care Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
چکیده
Introduction:
Chitosan, which is an amino polysaccharide resulting from the deacetylation of chitin, regarding its biomedical features such as antioxidant activity, muco-adhesive and hemostatic properties, antibacterial and anti-inflammatory effects, used for medical purposes. Oral cavity and oropharynx are two important structures that in terms of rich blood supply, hemorrhage might be life threating requiring effective hemostatic management. In present study, we evaluated the effect of chitosan dressing on oral cavity wound healing and hemostasis.
 Materials and Methods:
Nine male dogs were selected simple randomized and divided into three groups. A wound was made in the buccal mucosa bilaterally. We used chitosan powder dressing on the right side, chitosan-free gaze was used on the left side and bleeding time was determined. Three Dogs after5, three dogs after 10, and three dogs after15 days underwent biopsy bilaterally and pathologic assessment performed. Continuous and ordinal variables were reported as a median and IQR, and Wilcoxon test, and Friedman test were used to analyzing. Data were analyzed using SPSS 21.
Results:
Statistical analysis (Wilcoxon test) showed that the overall differences between two groups were statistically significant for (Acute inflammation score: P=.025; Collagenization score: P=.046; Neovascularization score: P=.046; Granulation tissue: P=0.046, and Re-epithelialization score: P=0.038). Chitosan powder dressing application significantly reduced acute inflammation and neovascularization, and increased collagenization, granulation tissue and re-epithelialization.  Furthermore, the median time of bleeding and percentage change of wound size which were not statistically significant for all 3days in the case and control groups but they were clinically significant.
Conclusion:
Chitosan salt powder dressing positively impacts the wound bleeding control and on mucosal wound healing according to histopathologic and gross wound healing indexes.
کلیدواژه‌ها
Chitosan؛ Bleeding time؛ Mouth mucosa؛ Wound healing؛ Hemorrhage
مراجع
  1. Mehrabani MG, Karimian R, Rakhshaei R, Pakdel F, Eslami H, Fakhrzadeh V, et al. Chitin/silk fibroin/ TiO(2) bio-nanocomposite as a biocompatible wound dressing bandage with strong antimicrobial activity. International journal of biological macromolecules. 2018;116:966-76. Epub 2018/ 05/ 22. doi: 10.1016/j.ijbiomac.2018.05.102. PubMed PMID: 29782987.
  2. Nwe N, Furuike T, Tamura H. Isolation and characterization of chitin and chitosan from marine origin. Advances in food and nutrition research. 2014;72:1-15. Epub 2014/08/02. doi: 10.1016/b978-0-12-800269-8.00001-4. PubMed PMID:25081074.
  3. McGrath B, McCarthy S, Sam K, Wold A, Stolten M, Bennett A. Biocompatible and bioabsorbable derivatized chitosan compositions. Google Patents; 2017.
  4. Ghimire S, Sarkar P, Rigby K, Maan A, Mukherjee S, Crawford KE, et al. Polymeric Materials for Hemostatic Wound Healing. Pharmaceutics. 2021;13(12). Epub 2021/12/29. doi: 10.3390/pharmaceutics13122127. PubMed PMID: 34959408; PubMed Central PMCID: PMCPMC 8708336.
  5. Feng P, Luo Y, Ke C, Qiu H, Wang W, Zhu Y, et al. Chitosan-Based Functional Materials for Skin Wound Repair: Mechanisms and Applications. Frontiers in bioengineering and biotechnology. 2021;9:650598. Epub 2021/03/09. doi: 10. 3389/ fbioe.2021.650598. PubMed PMID:3368 1176; PubMed Central PMCID: PMCPMC 7931995.
  6. Mizuno K, Yamamura K, Yano K, Osada T, Saeki S, Takimoto N, et al. Effect of chitosan film containing basic fibroblast growth factor on wound healing in genetically diabetic mice. Journal of Biomedical Materials Research Part A: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials. 2003;64(1):177-81.
  7. Mun MJ, Lee C-H, Lee B-J, Lee J-C, Jang JY, Jung SH, et al. Histopathologic Evaluations of the Lingual Artery in Healthy Tongue of Adult Cadaver. Clin Exp Otorhinolaryngol. 2016;9(3):257-62. Epub 2016/ 06/18. doi: 10.21053/ceo.2015.01137. Pub Med PMID: 27334510.
  8. Shahbazi A, Grimm A, Feigl G, Gerber G, Székely AD, Molnár B, et al. Analysis of blood supply in the hard palate and maxillary tuberosity-clinical implications for flap design and soft tissue graft harvesting (a human cadaver study). Clinical oral investigations. 2019;23(3):1153-60. Epub 2018/07/02. doi: 10.1007/s00784-018-2538-3. Pub Med PMID: 29961140.
  9. Iravani K, Mehravar S, Bahador M, Azarpira N. The Healing Effect of Amniotic Membrane in Laryngeal Defects in Rabbit Model. The Laryngoscope. 2021;131(2):E527-e33. Epub 2020/ 05/20. doi: 10.1002/lary.28745. PubMed PMID: 32427373.
  10. Abramov Y, Golden B, Sullivan M, Botros SM, Miller JJ, Alshahrour A, et al. Histologic characterization of vaginal vs. abdominal surgical wound healing in a rabbit model. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society. 2007;15(1):80-6. Epub 2007/01/25. doi: 10. 1111/j.1524-475X.2006.00188.x. PubMed PMID: 17244323.
  11. Carles G, Dabiri C, McHirgui A, Saoudi EO, Hcini N, Pouget K, et al. Uses of chitosan for treating different forms of serious obstetrics hemorrhages. Journal of gynecology obstetrics and human reproduction. 2017;46(9):693-5. Epub 2017/ 09/03. doi: 10.1016/j.jogoh.2017.08.003. PubMed PMID: 28864269.
  12. Carles G, Dabiri C, Mchirgui A. Different use of Chitosan for treating serious obstetric hemorrhages. Gynecol Obstet Res Open J. 2016;3:13-5.
  13. Ishizuka T, Hayashi T, Ishihara M, Yoshizumi Y, Aiko S, Nakamura S, et al. Submucosal injection, for endoscopic mucosal resection, of photocrosslinkable chitosan hydrogel in DMEM/ F12 medium. Endoscopy. 2007;39(5):428-33. Epub 2007/05/23. doi: 10.1055/s-2007-966393. PubMed PMID: 17516349.
  14. Chung Y-J, An S-Y, Yeon J-Y, Shim WS, Mo J-H. Effect of a Chitosan Gel on Hemostasis and Prevention of Adhesion After Endoscopic Sinus Surgery. Clin Exp Otorhinolaryngol. 2016;9(2):143-9. Epub 2016/04/19. doi: 10.21053/ceo.2015.00591. PubMed PMID: 27090275.
  15. Zhou JC, Zhang JJ, Zhang W, Ke ZY, Zhang B. Efficacy of chitosan dressing on endoscopic sinus surgery: a systematic review and meta-analysis. European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery. 2017; 274(9):3269-74. Epub 2017/05/01. doi: 10. 1007 / s00405-017-4584-x. PubMed PMID: 28456847.
  16. Deineka V, Sulaieva O, Pernakov N, Radwan-Pragłowska J, Janus L, Korniienko V, et al. Hemostatic performance and biocompatibility of chitosan-based agents in experimental parenchymal bleeding. Materials science & engineering C, Materials for biological applications. 2021; 120: 111740. Epub 2021/02/07. doi: 10. 1016/j. msec. 2020.111740. PubMed PMID: 33545883.
  17. Zhao WY, Fang QQ, Wang XF, Wang XW, Zhang T, Shi BH, et al. Chitosan-calcium alginate dressing promotes wound healing: A preliminary study. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society. 2020;28(3):326-37. Epub 2019/12/24. doi: 10.1111/wrr.12789. Pu bMed PMID: 31868976.
  18. Chen X, Zhang M, Wang X, Chen Y, Yan Y, Zhang L, et al. Peptide-modified chitosan hydrogels promote skin wound healing by enhancing wound angiogenesis and inhibiting inflammation. American journal of translational research. 2017; 9(5): 2352-62. Epub 2017/06/01. PubMed PMID: 28 559985; PubMed Central PMCID: PMCPMC 5446517.
  19. Saini S, Dhiman A, Nanda S. Immunomodulatory Properties of Chitosan: Impact on Wound Healing and Tissue Repair. Endocrine, metabolic & immune disorders drug targets. 2020; 20(10):1611-23. Epub 2020/05/04. doi: 10. 2174/ 1871530320666200503054605. PubMed PMID: 32359344.
  20. Mahattanadul S, Mustafa MW, Kuadkaew S, Pattharachayakul S, Ungphaiboon S, Sawanyawisuth K. Oral ulcer healing and anti-Candida efficacy of an alcohol-free chitosan-curcumin mouthwash. European review for medical and pharmacological sciences. 2018;22(20):7020-3. Epub 2018/11/08. doi: 10. 26355/ eurrev_201810 _ 16173. PubMed PMID: 30402869.
  21. Tavaria F, Jorge MP, Ruiz LT, Pintado M, Carvalho JE. Anti-proliferative, anti-inflammatory, anti-ulcerogenic and wound healing properties of chitosan. Current Bioactive Compounds. 2016;25.
  22. Filho LBC, Silva GAB, Goes AM, de Abreu FAM, Assis MHS, Oliveira ASD, et al. Chitosan-based biomaterial and hyaluronic acid on the repair of intrabuccal bone defects in rats. Journal of the International Academy of Periodontology. 2021; 23(2): 138-49. Epub 2021/05/01. PubMed PMID: 33929813.
  23. Gupta A, Rattan V, Rai S. Efficacy of Chitosan in promoting wound healing in extraction socket: A prospective study. Journal of oral biology and craniofacial research. 2019;9(1):91-5. Epub 2018/ 11/21. doi: 10.1016/j.jobcr.2018.11.001. PubMed PMID: 30456164; PubMed Central PMCID: PMCPMC6234520.
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