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Munusamy, Suresh, Karthikeyan, Palanisamy, Kaliamoorthy, Sriram, Vadivel, Pullar. (1404). Biomimetic chitosan/alginate with zinc and strontium hydroxyapatite for periodontal regeneration application. , 13(1), 170-182. doi: 10.22038/nmj.2025.84635.2133
Suresh Munusamy; Palanisamy Karthikeyan; Sriram Kaliamoorthy; Pullar Vadivel. "Biomimetic chitosan/alginate with zinc and strontium hydroxyapatite for periodontal regeneration application". , 13, 1, 1404, 170-182. doi: 10.22038/nmj.2025.84635.2133
Munusamy, Suresh, Karthikeyan, Palanisamy, Kaliamoorthy, Sriram, Vadivel, Pullar. (1404). 'Biomimetic chitosan/alginate with zinc and strontium hydroxyapatite for periodontal regeneration application', , 13(1), pp. 170-182. doi: 10.22038/nmj.2025.84635.2133
Munusamy, Suresh, Karthikeyan, Palanisamy, Kaliamoorthy, Sriram, Vadivel, Pullar. Biomimetic chitosan/alginate with zinc and strontium hydroxyapatite for periodontal regeneration application. , 1404; 13(1): 170-182. doi: 10.22038/nmj.2025.84635.2133

Biomimetic chitosan/alginate with zinc and strontium hydroxyapatite for periodontal regeneration application

Nanomedicine Journal
دوره 13، شماره 1، فروردین 2026، صفحه 170-182    اصل مقاله (1.19 M)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22038/nmj.2025.84635.2133
نویسندگان
Suresh Munusamy1، 2؛ Palanisamy Karthikeyan3؛ Sriram Kaliamoorthy4؛ Pullar Vadivel* 5
1Department of Chemistry, Sri Sarada Niketan College of Arts and Science for women, Salem, India
2Department of Chemistry, Periyar University, Salem, India
3Department of Chemistry, Government Arts and Science College Idappadi, Salem- 637102, India.
4Vinayaka Mission's Medical College and Hospital, Vinayaka Mission's Research Foundation (Deemed to be University), Karaikal, Puducherry, India, 609609.
5Department of Chemistry, Salem Sowdeswari College, Salem-636010.
چکیده
Objective(s): To evaluate the potential of a novel alginate/chitosan/Zinc-strontium hydroxyapatite (AG/CS/SHA) composite scaffold for periodontal tissue regeneration by assessing its biocompatibility, bioactivity, and degradation characteristics.
Materials and Methods: An AG/CS/SHA composite scaffold was fabricated using a freeze-drying technique. Characterization involved Fourier Transform Infrared Spectroscopy (FTIR) to confirm chemical composition, X-ray Diffraction (XRD) to analyze crystallinity, Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) to investigate microstructure and surface morphology. In vitro studies assessed biocompatibility using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, cell viability with Acridine Orange/Propidium Iodide (AO/PI) staining, alkaline phosphatase (ALP) activity, and calcium deposition to evaluate osteogenic differentiation.
Results: FTIR and XRD confirmed SHA incorporation. SEM/TEM revealed a porous structure with uniform SHA distribution. The composite exhibited controlled swelling and degradation. MTT assay and AO/PI staining demonstrated good cell viability. ALP activity (20% higher) and ARS staining (35% increase) were significantly higher in Zn and Sr in HA within AG/CS (AG/CS/SHA) scaffolds compared to AG/CS (control), indicating enhanced osteogenic differentiation and mineralization.
Conclusion: The results demonstrate that the AG/CS/SHA composite possesses favorable characteristics for bone tissue engineering applications, including excellent biocompatibility, suitable mechanical properties, and the ability to promote osteogenic differentiation. These findings suggest that the AG/CS/SHA scaffold holds significant promise as a promising biomaterial for periodontal tissue regeneration, providing a supportive environment for cell growth, differentiation, and new tissue formation.
کلیدواژه‌ها
Periodontal Regeneration؛ Biocomposites؛ Hydroxyapatites؛ Tissue Engineering؛ Osteogenesis
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آمار
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