سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Lister, I Nyoman, Chiuman, Linda, Mutia, Maya, Hartono, Hartono, Girsang, Ermi, Sutendi, Annisa, Kusuma, Hanna, Hadiprasetyo, Dhanar, Widowati, Wahyu. (1404). Hepatoprotective effects of Curcuma xanthorrhiza Roxb. extract via free radical scavenger, inhibiting apoptosis and inflammation mechanisms in acetaminophen-induced liver injury. , 28(8), 1100-1106. doi: 10.22038/ijbms.2025.82500.17833
I Nyoman Ehrich Lister; Linda Chiuman; Maya Sari Mutia; Hartono Hartono; Ermi Girsang; Annisa Firdaus Sutendi; Hanna Sari Widya Kusuma; Dhanar Septyawan Hadiprasetyo; Wahyu Widowati. "Hepatoprotective effects of Curcuma xanthorrhiza Roxb. extract via free radical scavenger, inhibiting apoptosis and inflammation mechanisms in acetaminophen-induced liver injury". , 28, 8, 1404, 1100-1106. doi: 10.22038/ijbms.2025.82500.17833
Lister, I Nyoman, Chiuman, Linda, Mutia, Maya, Hartono, Hartono, Girsang, Ermi, Sutendi, Annisa, Kusuma, Hanna, Hadiprasetyo, Dhanar, Widowati, Wahyu. (1404). 'Hepatoprotective effects of Curcuma xanthorrhiza Roxb. extract via free radical scavenger, inhibiting apoptosis and inflammation mechanisms in acetaminophen-induced liver injury', , 28(8), pp. 1100-1106. doi: 10.22038/ijbms.2025.82500.17833
Lister, I Nyoman, Chiuman, Linda, Mutia, Maya, Hartono, Hartono, Girsang, Ermi, Sutendi, Annisa, Kusuma, Hanna, Hadiprasetyo, Dhanar, Widowati, Wahyu. Hepatoprotective effects of Curcuma xanthorrhiza Roxb. extract via free radical scavenger, inhibiting apoptosis and inflammation mechanisms in acetaminophen-induced liver injury. , 1404; 28(8): 1100-1106. doi: 10.22038/ijbms.2025.82500.17833

Hepatoprotective effects of Curcuma xanthorrhiza Roxb. extract via free radical scavenger, inhibiting apoptosis and inflammation mechanisms in acetaminophen-induced liver injury

Iranian Journal of Basic Medical Sciences
مقاله 13، دوره 28، شماره 8، آبان 2025، صفحه 1100-1106    اصل مقاله (507.06 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2025.82500.17833
نویسندگان
I Nyoman Ehrich Lister1؛ Linda Chiuman1؛ Maya Sari Mutia1؛ Hartono Hartono1؛ Ermi Girsang1؛ Annisa Firdaus Sutendi2؛ Hanna Sari Widya Kusuma2؛ Dhanar Septyawan Hadiprasetyo2، 3؛ Wahyu Widowati* 4
1Faculty of Medicine, Universitas Prima Indonesia, Medan 20118, North Sumatera, Indonesia
2Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, Indonesia
3Faculty of Pharmacy, Jenderal Achmad Yani University, Cimahi 40531, Indonesia
4Faculty of Medicine, Maranatha Christian University, Bandung 40164, West Java, Indonesia
چکیده
Objective(s): Acetaminophen (APAP)-mediated liver injury poses a significant public health concern. Curcuma xanthorrhiza extract (CXE) has been traditionally used for its hepatoprotective properties. This research aimed to assess the hepatoprotective effects of CXE in APAP-mediated hepatotoxicity by investigating the modulatory effects of CXE on key biomarkers, including Interleukin (IL), namely, (IL-6), IL-10, IL-1β, Nitric Oxide (NO), Lactate Dehydrogenase (LDH), and the genes expression related to apoptosis-like Caspase-3 (Casp-3), Casp-9, and genes related to liver metabolic c-Jun N-terminal Kinase (JNK), in APAP-mediated HepG2 cells.
Materials and Methods: APAP-induced HepG2 cells were treated with different concentrations of CXE. IL-6, IL-10, IL were measured using an Enzyme-linked Immunosorbent Assay (ELISA) and NO, LDH were measured using colorimetric assay. Gene expression was analyzed using quantitative Real-Time Reverse Transcription (qRT-PCR).
Results: CXE significantly reduced IL-1β and IL-6 levels, enhanced IL-10 production, and attenuated NO levels in APAP-mediated hepatotoxicity. CXE also suppressed the expression of Casp-9, Casp-3, JNK, and LDH levels. The study presented a concentration-dependent response, with 125 μg/ml CXE exhibiting the most pronounced effects. CXE effectively modulated immune responses, decreased oxidative stress, and inhibited apoptotic and inflammatory pathways in APAP-mediated hepatotoxic cells.
Conclusion: These studies highlight the CXE potential as a therapeutic candidate for liver disorders, particularly in drug-mediated liver injury.
کلیدواژه‌ها
Acetaminophen؛ Antioxidants؛ Apoptosis؛ Curcuma xanthorrhiza؛ Free radical scavengers Liver injury
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