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Baghban, Arezoo, Momen Heravi, Mohammad, Rezaee, Seyed Abdolrahim, Tafaghodi, Mohsen, Bozorgmehr, Mohammadreza. (1404). Nanoencapsulation of B-toxin from herbal extracts: Targeting HTLV-1 protease and ATLL. , 28(8), 1047-1056. doi: 10.22038/ijbms.2025.83900.18154
Arezoo Baghban; Mohammad Momen Heravi; Seyed Abdolrahim Rezaee; Mohsen Tafaghodi; Mohammadreza Bozorgmehr. "Nanoencapsulation of B-toxin from herbal extracts: Targeting HTLV-1 protease and ATLL". , 28, 8, 1404, 1047-1056. doi: 10.22038/ijbms.2025.83900.18154
Baghban, Arezoo, Momen Heravi, Mohammad, Rezaee, Seyed Abdolrahim, Tafaghodi, Mohsen, Bozorgmehr, Mohammadreza. (1404). 'Nanoencapsulation of B-toxin from herbal extracts: Targeting HTLV-1 protease and ATLL', , 28(8), pp. 1047-1056. doi: 10.22038/ijbms.2025.83900.18154
Baghban, Arezoo, Momen Heravi, Mohammad, Rezaee, Seyed Abdolrahim, Tafaghodi, Mohsen, Bozorgmehr, Mohammadreza. Nanoencapsulation of B-toxin from herbal extracts: Targeting HTLV-1 protease and ATLL. , 1404; 28(8): 1047-1056. doi: 10.22038/ijbms.2025.83900.18154

Nanoencapsulation of B-toxin from herbal extracts: Targeting HTLV-1 protease and ATLL

Iranian Journal of Basic Medical Sciences
مقاله 8، دوره 28، شماره 8، آبان 2025، صفحه 1047-1056    اصل مقاله (839.81 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2025.83900.18154
نویسندگان
Arezoo Baghban1؛ Mohammad Momen Heravi1؛ Seyed Abdolrahim Rezaee* 1، 2؛ Mohsen Tafaghodi1، 3؛ Mohammadreza Bozorgmehr1
1Department of Chemistry, Mashhad Branch, Islamic Azad University, Mashhad, Iran
2Immunology Research Center, Inflammation and Inflammatory Diseases Division, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): Toxin B and isotoxin B (TB, isoTB) are major constituents of the Taxus baccata tree. This study investigates the inhibitory effect of TB and isoTB on adult T-cell leukemia/lymphoma (ATLL), particularly on human T-lymphotropic virus type 1 protease (HTLV-1 PR). HTLV-1 protease (HTLV-1 PR) is an aspartic acid protease and a promising therapeutic target for human immunodeficiency virus (HIV) PR inhibitors. 
Materials and Methods: The anticancer properties of T. baccata plant components encapsulated in PLGA nanoparticles (NPs/ PLGA/TB) were evaluated by in vitro assays using different cell lines. Cancerous cell lines, including HTLV-1-infected-MT2, were treated with varying concentrations of TB and alcoholic extract, and a combined peptide was designed and expressed using recombined NPs/PLGA/TB in a human Fc gamma1 (HTLV-1 PR: hFc gamma1) against HTLV-1. 
Results: Our results show that the viability of cancer cells after NPs/ PLGA/TB treatment significantly decreased in a time- and dose-dependent manner using the MTT assay. The inhibitory effect of NPs/ PLGA/TB on the HTLV-1-infected-MT2 cell line, in the absence of recombinant peptide, was (38.98 ± 0.23) and in the presence was (16.18 ± 2.03) in 72 hr (P<0.001). This indicates a double inhibitory effect in the presence of the peptide. The enzymatic effect of HTLV-1-protease on its fluorochrome substrate in the presence of TB and isoTB showed nearly complete enzyme inhibition. 
Conclusion: These findings present a promising avenue for introducing therapeutic agents with anticancer properties to treat progressive cancers, such as viral ATLL, and inducing effective antiviral responses.
کلیدواژه‌ها
Cancer؛ Human T-cell leukemia - virus type 1 (HTLV-1)؛ Nanoparticles؛ Paclitaxel؛ Poly lactic-co-glycolic acid - (PLGA)؛ Retrovirus
مراجع
1. Xie L, Zhu G, Shang J, Chen X, Zhang C, Ji X, et al. An overview on the biological activity and anti-cancer mechanism of lovastatin. Cell Signal 2021;87:110122.
2. Grobosch T, Schwarze B, Stoecklein D, Binscheck T. Fatal poisoning with Taxus baccata. Quantification of paclitaxel (taxol A), 10-Deacetyltaxol, baccatin III, 10-deacetylbaccatin III, cephalomannine (taxol B), and 3, 5-dimethoxyphenol in body fluids by liquid chromatography–tandem mass spectrometry. J Anal Toxicol 2012;36:36-43.
3. Thomas P, Polwart A. Taxus baccata L. J Ecology 2003;91:489-524.
4. Ahmadi Ghezeldasht S, Shamsian SAA, Gholizadeh Navashenaq J, Miri R, Ashrafi F, Mosavat A, et al. HTLV‐1 oncovirus‐host interactions: From entry to the manifestation of associated diseases. Rev Med Virol 2021;31:e2235.
5. Voshavar C. Protease inhibitors for the treatment of HIV/AIDS: Recent advances and future challenges. Curr Top Med Chem 2019;19:1571-98.
6. Freed EO. HIV-1 replication. Somat Cell Mol Genet 2001;26:13-33.
7. Kochman J, Jakubczyk K, Antoniewicz J, Mruk H, Janda K. Health benefits and chemical composition of matcha green tea: A review. Molecules 2020;26:85-95.
8. Wilson CR, Hooser SB. Toxicity of yew (Taxus spp.) alkaloids.  Veterinary toxicology: Elsevier; 2018. p. 947-54.
9. Essien ER, Atasie VN, Udobang EU, Umanu G. Preparation of monodispersed and cytotoxic silver nanoparticles using Launaea taraxacifolia leaf extract. J Nanostructure Chem 2019;9:259-268.
10. Cortinovis C, Caloni F. Epidemiology of intoxication of domestic animals by plants in Europe. Vet J 2013;197:163-168.
11.    Jafarzadeh S, Nooshkam M, Zargar M, Garavand F, Ghosh S, Hadidi M, et al. Green synthesis of nanomaterials for smart biopolymer packaging: challenges and outlooks. J Nanostructure  Chem 2024;14:113-136.
12. FitzGerald D, Pastan I. Targeted toxin therapy for the treatment of cancer. J Natl Cancer Inst 1989;81:1455-1463.
13. Rajabi S, Shakib H, Safari N, Maresca M, Hamzelo Moghadam M. Targeting autophagy for breast cancer prevention and therapy: From classical methods to phytochemical agents. Iran J Basic Med Sci 2024;27:1475-1491.
14. Kratz F, Warnecke A. Finding the optimal balance: Challenges of improving conventional cancer chemotherapy using suitable combinations with nano-sized drug delivery systems. J Control Release 2012;164:221-235.
15. Dongsar TS, Dongsar TT, Gupta G, Alsayari A, Wahab S, Kesharwani P. PLGA nanomedical consignation: A novel approach for the management of prostate cancer. Inter J Pharm 2024:123808.
16. Rocha CV, Gonçalves V, da Silva MC, Bañobre-López M, Gallo J. PLGA-based composites for various biomedical applications. Int J Mol Sci 2022;23:2034-2066.
17. Jelvehgari M, Barar J, Valizadeh H, HEYDARI N. Preparation and evaluation of poly (ε-caprolactone) nanoparticles-in-microparticles by W/O/W emulsion method. 2010; 13:85-96.
18. Luedemann A, Strassburg K, Erban A, Kopka J. TagFinder for the quantitative analysis of gas chromatography—mass spectrometry (GC-MS)-based metabolite profiling experiments. Bioinformatics 2008;24:732-737.
19. Hiller K, Hangebrauk J, Jäger C, Spura J, Schreiber K, Schomburg D. MetaboliteDetector: comprehensive analysis tool for targeted and nontargeted GC/MS based metabolome analysis. Anal Chem 2009;81:3429-3439.
20. Niseteo T, Komes D, Belščak-Cvitanović A, Horžić D, Budeč M. Bioactive composition and antioxidant potential of different commonly consumed coffee brews affected by their preparation technique and milk addition. Food Chem 2012;134:1870-1877.
21. vom Saal FS, Akingbemi BT, Belcher SM, Crain DA, Crews D, Guidice LC, et al. Flawed experimental design reveals the need for guidelines requiring appropriate positive controls in endocrine disruption research. Toxicol Sci 2010;115:612-613.
22. Wolfender J-L. HPLC in natural product analysis: The detection issue. Planta Med 2009;75:719-734.
23. Eugster PJ, Guillarme D, Rudaz S, Veuthey J-L, Carrupt P-A, Wolfender J-L. Ultra high pressure liquid chromatography for crude plant extract profiling. J AOAC Int 2011;94:51-70.
24. Munteanu C, Kuemmerle T, Keuler NS, Müller D, Balázs P, Dobosz M, et al. Legacies of 19th century land use shape contemporary forest cover. Glob Environ Change 2015;34:83-94.
25. Guo Z, Liu Y, Luo Y. Mechanisms of carotenoid intestinal absorption and the regulation of dietary lipids: Lipid transporter-mediated transintestinal epithelial pathways. Crit Rev Food Sci Nutr 2024;64:1791-1816.
26. Swider E, Koshkina O, Tel J, Cruz LJ, de Vries IJM, Srinivas M. Customizing poly (lactic-co-glycolic acid) particles for biomedical applications. Acta Biomater 2018;73:38-51.
27. Bibi S, Kaur R, Henriksen-Lacey M, McNeil SE, Wilkhu J, Lattmann E, et al. Microscopy imaging of liposomes: from coverslips to environmental SEM. Int J Pharm 2011;417:138-150.
28. Hanaor DA, Assadi MH, Li S, Yu A, Sorrell CC. Ab initio study of phase stability in doped TiO2. Comput Mech 2012;50:185-194.
29. Feng Y, Yu B, Xu P, Zou M. The effective thermal conductivity of nanofluids based on the nanolayer and the aggregation of nanoparticles. J Phys D Appl Phys 2007;40:3164-3171.
30. Domínguez-Rodríguez G, Marina ML, Plaza M. Strategies for the extraction and analysis of non-extractable polyphenols from plants. J Chromatogr A 2017;1514:1-15.
31. Borra RC, Lotufo MA, Gagioti SM, Barros FdM, Andrade PM. A simple method to measure cell viability in proliferation and cytotoxicity assays. Braz Oral Res 2009;23:255-262.
32. Browning CH, Cohen JB, Ellingworth S, Gulbransen R. The antiseptic properties of the amino derivatives of styryl and anil quinoline. Proc R Soc Lond B Biol Sci 1926;100:293-325.
33. Vickers A, Goyal N, Harland R, Rees R. Do certain countries produce only positive results? A systematic review of controlled trials. Control Clin Trials 1998;19:159-166.
34. Michailidou G, Ainali NM, Xanthopoulou E, Nanaki S, Kostoglou M, Koukaras EN, et al. Effect of poly (vinyl alcohol) on nanoencapsulation of budesonide in chitosan nanoparticles via ionic gelation and its improved bioavailability. Polymers 2020;12:1101-1122.
35. Colovos C, Yeates TO. Verification of protein structures: Patterns of nonbonded atomic interactions. Protein Sci 1993;2:1511-1519.
36. Laskowski RA, MacArthur MW, Moss DS, Thornton JM. PROCHECK: A program to check the stereochemical quality of protein structures. J Appl Crystallogr 1993;26:283-291.
37. Ghezeldasht SA, Shirdel A, Assarehzadegan MA, Hassannia T, Rahimi H, Miri R, et al. Human T lymphotropic virus type I (HTLV-I) oncogenesis: molecular aspects of virus and host interactions in pathogenesis of adult T cell leukemia/lymphoma (ATL). Iran J Basic Med Sci 2013;16:179-195.
38. Ahmadi Ghezeldasht S, Blackbourn DJ, Mosavat A, Rezaee SA. Pathogenicity and virulence of human T lymphotropic virus type-1 (HTLV-1) in oncogenesis: adult T-cell leukemia/lymphoma (ATLL). Crit Rev Clin Lab Sci 2023;60:189-211.
39. Ishida T, Utsunomiya A, Iida S, Inagaki H, Takatsuka Y, Kusumoto S, et al. Clinical significance of CCR4 expression in adult T-cell leukemia/lymphoma: its close association with skin involvement and unfavorable outcome. Clin Cancer Res 2003;9:3625-3634.
40. Ohno N, Tani A, Uozumi K, Hanada S, Furukawa T, Akiba S, et al. Expression of functional lung resistance–related protein predicts poor outcome in adult T-cell leukemia. Blood 2001;98:1160-1165.
41. Baghban A, Rezaee SR, Tafaghodi M, Bozorgmehr M, Heravi MM. Combating HTLV-1 infections with Taxus baccata phytoconstituents: Molecular mechanisms potential anti-ATLL agents. Trop J Nat Prod Res 2024:100036.
42. Soleimanpour S, Hassannia T, Motiee M, Amini AA, Rezaee S. Fcγ1 fragment of IgG1 as a powerful affinity tag in recombinant Fc-fusion proteins: Immunological, biochemical and therapeutic properties. Crit Rev Biotechnol 2017;37:371-392.
43. Sultan H, Fesenkova VI, Addis D, Fan AE, Kumai T, Wu J, et al. Designing therapeutic cancer vaccines by mimicking viral infections. Cancer Immunol Immunother 2017;66:203-213.
44. Boulton S, Selvaratnam R, Blondeau J-P, Lezoualc’h F, Melacini G. Mechanism of selective enzyme inhibition through uncompetitive regulation of an allosteric agonist. J Am Chem Soc
2018;140:9624-9637.
45. Buonaguro L, Tagliamonte M. Peptide-based vaccine for cancer therapies. Front Immunol 2023;14:1210044.

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