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Hosseinpour, Leili, Baharara, Javad, Darroudi, Majid, Sabouri, Zahra, Haji Ghasem Kashani, Maryam, Zaker Bostanabad, Saeed. (1404). Green synthesis of selenium nanoparticles using Cordia myxa extract and assessment of their cytotoxic and antioxidant properties. , 15(5), 1531-1545. doi: 10.22038/ajp.2025.25954
Leili Hosseinpour; Javad Baharara; Majid Darroudi; Zahra Sabouri; Maryam Haji Ghasem Kashani; Saeed Zaker Bostanabad. "Green synthesis of selenium nanoparticles using Cordia myxa extract and assessment of their cytotoxic and antioxidant properties". , 15, 5, 1404, 1531-1545. doi: 10.22038/ajp.2025.25954
Hosseinpour, Leili, Baharara, Javad, Darroudi, Majid, Sabouri, Zahra, Haji Ghasem Kashani, Maryam, Zaker Bostanabad, Saeed. (1404). 'Green synthesis of selenium nanoparticles using Cordia myxa extract and assessment of their cytotoxic and antioxidant properties', , 15(5), pp. 1531-1545. doi: 10.22038/ajp.2025.25954
Hosseinpour, Leili, Baharara, Javad, Darroudi, Majid, Sabouri, Zahra, Haji Ghasem Kashani, Maryam, Zaker Bostanabad, Saeed. Green synthesis of selenium nanoparticles using Cordia myxa extract and assessment of their cytotoxic and antioxidant properties. , 1404; 15(5): 1531-1545. doi: 10.22038/ajp.2025.25954

Green synthesis of selenium nanoparticles using Cordia myxa extract and assessment of their cytotoxic and antioxidant properties

Avicenna Journal of Phytomedicine
دوره 15، شماره 5، آذر و دی 2025، صفحه 1531-1545    اصل مقاله (1.63 M)
نوع مقاله: Original Research Article
شناسه دیجیتال (DOI): 10.22038/ajp.2025.25954
نویسندگان
Leili Hosseinpour1؛ Javad Baharara1؛ Majid Darroudi* 2؛ Zahra Sabouri3؛ Maryam Haji Ghasem Kashani4؛ Saeed Zaker Bostanabad5
1Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
2Nuclear Medicine Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
3Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4Department of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran
5Department of Biology, Parand Branch, Islamic Azad University, Tehran, Iran
چکیده
Objective: This research aimed to explore the cytotoxic, apoptotic, and antioxidant effects of selenium nanoparticles (Se-NPs) produced by Cordia myxa (C. myxa) aqueous extract via a green reduction method.
Materials and Methods: To synthesize Se-NPs, Na2SeO3 and C. myxa plant extract were used and their anticancer and antioxidant effects were investigated. After the synthesis of Se-NPs, their physicochemical properties were investigated by various techniques such as UV-Vis, XRD, TEM, FESEM/PSA, EDX, FT-IR, and DLS/Zeta. Next, the lethality of Se-NPs on cancerous Huh-7 and normal L929 cells was investigated. Then, using Annexin V/PI and DAPI kits, the number of apoptotic cells in terms of the obtained percentage and the amount of ROS production was determined by flow cytometry. The influence of Se-NPs on the expression of genes associated with apoptosis has been examined.
Results: The XRD pattern and FESEM/TEM images confirmed the successful production of Se-NPs with an amorphous nature and size average of 11.9 nm. Flow cytometry analyses revealed a significant increase in ROS levels after 24 h of treatment with Se-NPs, demonstrating a concentration-dependent effect. Conclusion: The findings indicate that Se-NPs exhibit anticancer activity against Huh-7 cells, as evidenced by the upregulation of Bax, p53, and Caspase3 gene expression. Therefore, it can be asserted that Se-NPs have the potential to eliminate cancer cells, while simultaneously providing a protective effect on normal cells.
کلیدواژه‌ها
Nanoparticle؛ Cancer؛ Apoptosis؛ Biosynthesis؛ C. myxa plant
آمار
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