سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Sudeep, Heggar, Sundeep, Kuppam, Venkatakrishna, Karempudi, Raj, Amrutha, Bhumika, Ranganath, Prasanna Kumar, Thammatadhahalli Parameshwarappa, Shyamprasad, Kodimule. (1404). PorelisTM, a standardized purple tea extract, improves adipocyte functionality by regulating the leptin and adiponectin levels in high-fat diet-induced C57Bl/6 mice. , 28(8), 1037-1046. doi: 10.22038/ijbms.2025.84192.18209
Heggar Venkataramana Sudeep; Kuppam Sundeep; Karempudi Venkatakrishna; Amrutha Raj; Ranganath Bhumika; Thammatadhahalli Parameshwarappa Prasanna Kumar; Kodimule Shyamprasad. "PorelisTM, a standardized purple tea extract, improves adipocyte functionality by regulating the leptin and adiponectin levels in high-fat diet-induced C57Bl/6 mice". , 28, 8, 1404, 1037-1046. doi: 10.22038/ijbms.2025.84192.18209
Sudeep, Heggar, Sundeep, Kuppam, Venkatakrishna, Karempudi, Raj, Amrutha, Bhumika, Ranganath, Prasanna Kumar, Thammatadhahalli Parameshwarappa, Shyamprasad, Kodimule. (1404). 'PorelisTM, a standardized purple tea extract, improves adipocyte functionality by regulating the leptin and adiponectin levels in high-fat diet-induced C57Bl/6 mice', , 28(8), pp. 1037-1046. doi: 10.22038/ijbms.2025.84192.18209
Sudeep, Heggar, Sundeep, Kuppam, Venkatakrishna, Karempudi, Raj, Amrutha, Bhumika, Ranganath, Prasanna Kumar, Thammatadhahalli Parameshwarappa, Shyamprasad, Kodimule. PorelisTM, a standardized purple tea extract, improves adipocyte functionality by regulating the leptin and adiponectin levels in high-fat diet-induced C57Bl/6 mice. , 1404; 28(8): 1037-1046. doi: 10.22038/ijbms.2025.84192.18209

PorelisTM, a standardized purple tea extract, improves adipocyte functionality by regulating the leptin and adiponectin levels in high-fat diet-induced C57Bl/6 mice

Iranian Journal of Basic Medical Sciences
مقاله 7، دوره 28، شماره 8، آبان 2025، صفحه 1037-1046    اصل مقاله (617.86 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2025.84192.18209
نویسندگان
Heggar Venkataramana Sudeep* 1؛ Kuppam Sundeep2؛ Karempudi Venkatakrishna2؛ Amrutha Raj2؛ Ranganath Bhumika2؛ Thammatadhahalli Parameshwarappa Prasanna Kumar2؛ Kodimule Shyamprasad2
1R&D Center for Excellence, Vidya Herbs Pvt Ltd., #14A, Jigani I phase, Bangalore- 560 105, Karnataka, India
2R&D Center for Excellence, Vidya Herbs Pvt Ltd., #14A, Jigani I phase, Bangalore- 560 105, Karnataka, India
چکیده
Objective(s): Purple tea variety of Camelia sinensis (L.) Kuntze (Theaceae), with its unique presence of anthocyanins and 1,2-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-β-D-glucose (GHG), has enormous potential in the weight management. Here, the weight loss mechanism of a standardized purple tea hydroalcoholic extract (PorelisTM, 3-5% GHG) is demonstrated in high-fat-diet-induced mice.  
Materials and Methods: Male C57Bl/6 mice (20±2 g) were divided into five groups (n=8). In a 12-week study, the control group was given a normal diet, and the other groups were given HFD (60 % kcal from fat) for 12 weeks. The treatment groups received Porelis at 25, 50, and 100 mg/kg body weight, PO. 
Results: Porelis treatment markedly reduced the body weight, fat pad weights, hepatic lipid accumulation, and oxidative stress in the HFD mice. The adipogenic markers, CCAAT/enhancer binding protein alpha (C/EBPα), Peroxisome proliferator-activated receptor gamma (PPAR-γ), and Sterol Regulatory Element Binding Protein 1 (SREBP1) were down-regulated in Porelis-treated mice compared to HFD model group (P<0.0001). Porelis treatment reduced the serum leptin level (P<0.0001) while increasing the adiponectin (P<0.01) in HFD mice. The circulating trimethylamine N-oxide (TMAO) (P<0.0001) and insulin (P<0.001) levels were significantly reduced in the Porelis groups compared to the HFD-alone group. In addition, Porelis markedly inhibited the in vitro pancreatic lipase activity (IC50 = 223.3 µg/ml).     
Conclusion: Porelis exerts antiobesity activity in mice by regulating adipogenesis and energy homeostasis. Our data encourages further validation of Porelis-mediated weight loss effects in human subjects. 
کلیدواژه‌ها
Adipocyte differentiation؛ GHG؛ Leptin؛ Purple tea؛ Weight loss
مراجع
1. Lee YS, Seo YH, Kim JY. Antiobesity effect of radish leaf extracts on high fat diet-induced obesity in mice. Korean J Food Sci Technol 2022; 54: 297-305.
2. Kim HS, Han EJ, Fernando IPS, Asanka Sanjeewa KK, Jayawardena TU, Kim HJ, et al. Anti-allergy effect of mojabanchromanol isolated from Sargassum horneri in bone marrow-derived cultured mast cells. Algal Res 2020; 48: 101898. 
3. Clark JE. Diet, exercise or diet with exercise: Comparing the effectiveness of treatment options for weight-loss and changes in fitness for adults (18-65 years old) who are overfat, or obese; Systematic review and meta-analysis. J Diabetes Metab Disord 2015; 14: 31-59.
4. Cha JY, Nepali S, Lee HY, Hwang SW, Choi SY, Yeon JM et al. Chrysanthemum indicum L. ethanol extract reduces high-fat diet-induced obesity in mice. Exp Ther Med 2018; 15: 5070-5076. 
5. Rashid K, Wachira FN, Nyabuga JN, Wanyonyi B, Murilla G, Isaac AO. Kenyan purple tea anthocyanins ability to cross the blood brain barrier and reinforce brain antioxidant capacity in mice. Nutr Neurosci 2014; 17: 178-185.
6. Shimoda H, Hitoe S, Nakamura S, Matsuda H. Purple tea and its extract suppress diet-induced fat accumulation in mice and human subjects by inhibiting fat absorption and enhancing hepatic carnitine palmitoyl transferase expression. Int J Biomed Sci 2015; 11: 67-75.   
7. Joshi R, Rana A, Kumar V, Kumar D, Padwad YS, Yadav SK et al. Anthocyanins enriched purple tea exhibits antioxidant, immunostimulatory and anticancer activities. J Food Sci Technol 2017; 54: 1953-1963. 
8. da Silva TBV, Dias MI, Pereira C, Mandim F, Ivanov M, Soković M et al. Purple tea: Chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice. Food Funct 2023; 14: 1761-1772. 
9. Nair AB, Jacob S. A simple practice guide for dose conversion between animals and human. J Basic Clin Pharm 2016; 7: 27-31.
10. Kim JH, Kim HJ, Park HW, Youn SH, Choi DY, Shin CS. Development of inhibitors against lipase and alpha-glucosidase from derivatives of monascus pigment. FEMS Microbiol Lett 2007; 276: 93-98. 
11. Blois MS. Antioxidant determinations using a stable free radical. Nature 1958; 181: 1199-1200. 
12. Marcocci L, Maguire JJ, Droy-Lefaix MT, Packer L. The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761. Biochem Biophys Res Commun 1994; 201: 748-755.
13. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999; 26: 1231-1237. 
14. Elizabeth K, Rao MNA. Oxygen radical scavenging activity of curcumin. Int J Pharmaceut 1990; 58: 237-240.  
15. Choi WH, Um MY, Ahn J, Jung CH, Park MK, Ha TY. Ethanolic extract of Taheebo attenuates increase in body weight and fatty liver in mice fed a high-fat diet. Molecules 2014; 19: 16013-16023.
16. Ghaben AL, Scherer PE. Adipogenesis and metabolic health. Nat Rev Mol cell Biol 2019; 20: 242-258.
17. Wang G, Wu B, Zhang L, Cui Y, Zhang B, Wang H. Laquinimod prevents adipogenesis and obesity by down-regulating PPAR-γ and C/EBPα through activating AMPK. ACS Omega 2020; 5: 22958-22965.
18. Gouthamchandra K, Sudeep HV, Ranganath B, Amritharaj, Basavegowda LH, Kumara TP, et al. Antiobesity potential of Porelis, a standardized extract of purple tea (Camellia sinensis), via modulation of obesity-related inflammation in RAW264.7 macrophages and anti-adipogenesis in 3T3-L1 cells. Trop J Pharm Res 2025; 24: 31-38.
19. Polyzos SA, Kountouras J, Mantzoros CS. Adipose tissue, obesity and non-alcoholic fatty liver disease. Minerva Endocrinol. 2017; 42: 92-108.
20. Vekic J, Zeljkovic A, Stefanovic A, Jelic-Ivanovic Z, Spasojevic-Kalimanovska V.. Obesity and dyslipidemia. Metabolism 2019; 92: 71-81.
21. Lavie CJ, Milani RV, Ventura HO. Obesity and cardiovascular disease: risk factor, paradox, and impact of weight loss. J Am Coll Cardiol 2009; 53: 1925-1932. 
22. Wang Z, Klipfell E, Bennett BJ, Koeth R, Levison BS, Dugar B et al. Gut flora metabolism of phosphatidylcholine promotes cardiovascular disease. Nature 2011; 472: 57-63.   
23. Heianza Y, Ma W, DiDonato JA, Sun Q, Rimm EB, Hu FB, et al. Long-term changes in gut microbial metabolite trimethylamine N-oxide and coronary heart disease risk. J Am Coll Cardiol 2020; 75: 763-772. 
24. Dehghan P, Farhangi MA, Nikniaz L, Nikniaz Z, Asghari-Jafarabadi M. Gut microbiota-derived metabolite trimethylamine N-oxide (TMAO) potentially increases the risk of obesity in adults: An exploratory systematic review and dose-response meta-analysis. Obes Rev 2020; 21: e12993.
25. Kubota N, Yano W, Kubota T, Yamauchi T, Itoh S, Kumagai H, et al. Adiponectin stimulates AMP-activated protein kinase in the hypothalamus and increases food intake. Cell Metab 2007; 6: 55-68. 
26. Guilherme A, Virbasius J, Puri V, Czech MP. Adipocyte dysfunctions linking obesity to insulin resistance and type 2 diabetes. Nat Rev Mol Cell Biol 2008; 9: 367-377.

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