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Zhuang, Shiwei, Chen, Qiang, Guo, Xiao, Zhao, Wenhai, Qiu, Ye. (1404). Mechanism of action of total saponin Achyranthes in treating knee osteoarthritis explored using network pharmacology and animal experimentation. , 28(6), 762-771. doi: 10.22038/ijbms.2025.83153.17974
Shiwei Zhuang; Qiang Chen; Xiao Guo; Wenhai Zhao; Ye Qiu. "Mechanism of action of total saponin Achyranthes in treating knee osteoarthritis explored using network pharmacology and animal experimentation". , 28, 6, 1404, 762-771. doi: 10.22038/ijbms.2025.83153.17974
Zhuang, Shiwei, Chen, Qiang, Guo, Xiao, Zhao, Wenhai, Qiu, Ye. (1404). 'Mechanism of action of total saponin Achyranthes in treating knee osteoarthritis explored using network pharmacology and animal experimentation', , 28(6), pp. 762-771. doi: 10.22038/ijbms.2025.83153.17974
Zhuang, Shiwei, Chen, Qiang, Guo, Xiao, Zhao, Wenhai, Qiu, Ye. Mechanism of action of total saponin Achyranthes in treating knee osteoarthritis explored using network pharmacology and animal experimentation. , 1404; 28(6): 762-771. doi: 10.22038/ijbms.2025.83153.17974

Mechanism of action of total saponin Achyranthes in treating knee osteoarthritis explored using network pharmacology and animal experimentation

Iranian Journal of Basic Medical Sciences
مقاله 9، دوره 28، شماره 6، شهریور 2025، صفحه 762-771    اصل مقاله (568.08 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2025.83153.17974
نویسندگان
Shiwei Zhuang1؛ Qiang Chen2؛ Xiao Guo3؛ Wenhai Zhao4؛ Ye Qiu* 2
1The Third Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun 130117, China
2Institute of College of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China
3Jilin Cancer Hospital, Changchun, Changchun 130012, China
4Affiliated Hospital of Changchun University of Chinese Medicine, Changchun 130021, China
چکیده
Objective(s): Knee osteoarthritis (KOA) is a persistent degenerative disease affecting the joints, significantly reducing the quality of life for individuals afflicted. This study explores the therapeutic effects of total  saponin Achranthes (TSA) on KOA rats and its underlying mechanism. 
Materials and Methods: Forty-eight rats were randomly assigned to six experimental groups: a blank control group, a model group, a sham-operated group, and a TSA treatment group (low, medium, and high dose), with eight rats in each group. The rats were treated continuously for four weeks. The degree of joint swelling was quantified, and the Lequesne MG score was evaluated. Network pharmacology approaches were employed to pinpoint potential TSA targets and related pathways for managing KOA. Additionally, histopathological examinations were conducted on the knee cartilage of the rats. Serum levels of TNF-α and IL-1β were assessed through the ELISA assay. 
Results: The network pharmacology results indicate that TSA may effectively treat KOA through the MAPK and PI3K/Akt signaling pathways. Moreover, TSA significantly decreased the serum concentrations of pro-inflammatory cytokines such as TNF-α and IL-1β, and  TSA down-regulated the P38 MAPK, PI3K/Akt, and NF-κB pathways, whereas the KOA model showed up-regulation. The treatment also significantly reduced MMP-9, MMP-13, and ADAMTS-5 protein levels.
Conclusion: TSA can potentially ameliorate inflammation, safeguard knee cartilage tissue, and alleviate symptoms of KOA by inhibiting the MAPK/Akt/NF-κB signaling pathway.
کلیدواژه‌ها
Achyranthes؛ Knee osteoarthritis؛ Anti-inflammatory؛ Pharmacology؛ Rats؛ Matrix metalloproteinase 9
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