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Hossainpour, Hadi, Alvandi, Hosna, Abiri, Ramin, Aghaz, Faranak, Alvandi, Amirhooshang, Arkan, Elham. (1404). Preparation and characterization of gelatin/ sodium alginate with thymus Kotschyanus hydrogel for wound healing, In Vitro study. , 12(3), 443-453. doi: 10.22038/nmj.2025.83422.2088
Hadi Hossainpour; Hosna Alvandi; Ramin Abiri; Faranak Aghaz; Amirhooshang Alvandi; Elham Arkan. "Preparation and characterization of gelatin/ sodium alginate with thymus Kotschyanus hydrogel for wound healing, In Vitro study". , 12, 3, 1404, 443-453. doi: 10.22038/nmj.2025.83422.2088
Hossainpour, Hadi, Alvandi, Hosna, Abiri, Ramin, Aghaz, Faranak, Alvandi, Amirhooshang, Arkan, Elham. (1404). 'Preparation and characterization of gelatin/ sodium alginate with thymus Kotschyanus hydrogel for wound healing, In Vitro study', , 12(3), pp. 443-453. doi: 10.22038/nmj.2025.83422.2088
Hossainpour, Hadi, Alvandi, Hosna, Abiri, Ramin, Aghaz, Faranak, Alvandi, Amirhooshang, Arkan, Elham. Preparation and characterization of gelatin/ sodium alginate with thymus Kotschyanus hydrogel for wound healing, In Vitro study. , 1404; 12(3): 443-453. doi: 10.22038/nmj.2025.83422.2088

Preparation and characterization of gelatin/ sodium alginate with thymus Kotschyanus hydrogel for wound healing, In Vitro study

Nanomedicine Journal
دوره 12، شماره 3، مهر 2025، صفحه 443-453    اصل مقاله (1.02 M)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22038/nmj.2025.83422.2088
نویسندگان
Hadi Hossainpour1؛ Hosna Alvandi2، 3؛ Ramin Abiri1، 4؛ Faranak Aghaz2؛ Amirhooshang Alvandi* 1، 5؛ Elham Arkan2
1Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
2Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
3Nanobiotechnology Department, Faculty of Innovative Science and Technology, Razi University Kermanshah, Iran
4Fertility and Infertility Research Center, Research Institute for Health Technology, Kermanshah University of Medical Sciences, Kermanshah, Iran
5Medical Technology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
چکیده
Objective(s): Gelatin / Sodium Alginate Thymus kotschyanus (GE/SA T. kotschyanus) hydrogel was prepared for use as a wound healing.
Materials and Methods: The hydrogel was prepared by casting and cross-linking method and the physicochemical and the antimicrobial properties of the hydrogels were determined.
Results: The GE/SA hydrogel showed high mechanical properties, while the addition of the extract to the hydrogel led to the relaxation of the polymer network and thus increased the elasticity of the dressing, and it had a good biodegradation. The compatibility of hydrogels with fibroblast cells was confirmed by MTT assay. Also, GE/SA T. kotschyanus hydrogels were found to be excellent Hemocompatibile. Moreover, these hydrogels exhibited the antibacterial activity against Pseudomonas aeruginosa, and Escherichia coli. In addition, these hydrogels showed the best antifungal effect against Candida albicans.
Conclusion:  The GE/SA T. kotschyanus hydrogels had the potential for use in wound dressing.
کلیدواژه‌ها
Hydrogel؛ Gelatin؛ Sodium Alginate؛ Thymus kotschyanus؛ Wound healing
مراجع
1.    Zhang L, Yin H, Lei X, Lau JN, Yuan M, Wang X, Zhang F, Zhou F, Qi S, Shu B, Wu J. A systematic review and meta-analysis of clinical effectiveness and safety of hydrogel dressings in the management of skin wounds. Front Bioeng Biotechnol. 2019;7:342.
2.    Zhang X, Qin M, Xu M, Miao F, Merzougui C, Zhang X, Wei Y, Chen W, Huang D. The fabrication of antibacterial hydrogels for wound healing. Eur Polym J. 2021;146:110268. 
3.    Guo SA, DiPietro LA. Factors affecting wound healing. J Dent Res. 2010;89(3):219-229. 
4.    Zhang L, Ma Y, Pan X, Chen S, Zhuang H, Wang S. A composite hydrogel of chitosan/heparin/poly (γ-glutamic acid) loaded with superoxide dismutase for wound healing. Carbohydr Polym. 2018;180:168-174. 
5.    Ahmadian Z, Correia A, Hasany M, Figueiredo P, Dobakhti F, Eskandari MR, Hosseini SH, Abiri R, Khorshid S, Hirvonen J, Santos HA. A hydrogen‐bonded extracellular matrix‐mimicking bactericidal hydrogel with radical scavenging and hemostatic function for pH‐responsive wound healing acceleration. Adv Healthc Mater. 2021;10(3):2001122.
6.    Liang X, Huang C, Liu H, Chen H, Shou J, Cheng H, Liu G. Natural hydrogel dressings in wound care: Design, advances, and perspectives. Chin Chem Lett. 2024;35(10):109442.
7.    Stoica AE, Chircov C, Grumezescu AM. Hydrogel dressings for the treatment of burn wounds: an up-to-date overview. Materials. 2020;13(12):2853.
8.    Li Z, Liu H, Liao Y, Wang H, Sun X, Chen X, Yan H, Lin Q. Design and properties of alginate/gelatin/cellulose nanocrystals interpenetrating polymer network composite hydrogels based on in situ cross-linking. Eur Polym J. 2023;201:112556.
9.    Jadach B, Świetlik W, Froelich A. Sodium alginate as a pharmaceutical excipient: novel applications of a well-known polymer. J Pharm Sci. 2022;111(5):1250-61. 
10.    Afjoul H, Shamloo A, Kamali A. Freeze-gelled alginate/gelatin scaffolds for wound healing applications: An in vitro, in vivo study. Mater Sci Eng C. 2020;113:110957. 
11.    Hosseinzadeh S, Jafarikukhdan A, Hosseini A, Armand R. The application of medicinal plants in traditional and modern medicine: a review of Thymus vulgaris. Int J Clin Exp Med. 2015;6(9):635-642.
12.    Ghasemi G, Alirezalu A, Ghosta Y, Jarrahi A, Safavi SA, Abbas-Mohammadi M, Barba FJ, Munekata PE, Domínguez R, Lorenzo JM. Composition, antifungal, phytotoxic, and insecticidal activities of Thymus kotschyanus essential oil. Molecules. 2020 Mar 4;25(5):1152. 
13.    Nickavar B, Mojab F, Dolat-Abadi R. Analysis of the essential oils of two Thymus species from Iran. Food Chem. 2005;90(4):609-611. 
14.    Bagci E, Başer KH. Study of the essential oils of Thymus haussknechtii Velen and Thymus kotschyanus Boiss. et Hohen var. kotschyanus (Lamiaceae) taxa from the eastern Anatolian region in Turkey. Flavour Fragr J. 2005;20(2):199-202. 
15.    Mahmoudi M, Morteza-Semnani K, Mojra E. Anti-inflammatory and antinociceptive activity of Thymus pubescens extract. Fitoterapia. 2008;79(5):361-365.
16.    Baharfar R, Azimi R, Mohseni M. Antioxidant and antibacterial activity of flavonoid-, polyphenol-and anthocyanin-rich extracts from Thymus kotschyanus boiss & hohen aerial parts. J Food Sci Technol. 2015;52:6777-6783.
17.    Gulla S, Reddy MC, Reddy VC, Chitta S, Bhanoori M, Lomada D. Role of thymus in health and disease. Int Rev Immunol. 2023;42(5):347-363.
18.    Joshi A, Prasad SK, Joshi VK, Hemalatha S. Phytochemical standardization, antioxidant, and antibacterial evaluations of Leea macrophylla: a wild edible plant. J Food Drug Anal. 2016;24(2):324-331.
19.    Wang T, Zhu XK, Xue XT, Wu DY. Hydrogel sheets of chitosan, honey and gelatin as burn wound dressings. Carbohydr Polym. 2012;88(1):75-83.
20.    Pang JH, Wischke C, Lendlein A. In vitro degradation analysis of 3d-architectured gelatin-based hydrogels. MRS Adv. 2020;5(12-13):633-642.
21.    Delmar, K. and H. Bianco-Peled, Delmar K, Bianco-Peled H. Composite chitosan hydrogels for extended release of hydrophobic drugs. Carbohydr Polym. 2016;136: 570-580.
22.    Massana Roquero D, Bollella P, Katz E, Melman A. Controlling porosity of calcium alginate hydrogels by interpenetrating polyvinyl alcohol–diboronate polymer network. ACS Appl Polym Mater. 2021;3(3):1499-1507.
23.    Zhou G, Ruhan A, Ge H, Wang L, Liu M, Wang B, Su H, Yan M, Xi Y, Fan Y. Research on a novel poly (vinyl alcohol)/lysine/vanillin wound dressing: Biocompatibility, bioactivity and antimicrobial activity. Burns. 2014;40(8):1668-1678.
24.    Perkasa DP, Erizal E, Purwanti T, Tontowi AE. Characterization of semi-interpenetrated network alginate/gelatin wound dressing crosslinked at sol phase. Indones J Chem. 2018;18(2):367-375.
25.    Nair LS, Laurencin CT. Biodegradable polymers as biomaterials. Prog Polym Sci. 2007;32(8-9):762-798. 
26.    Alizadeh M, Abbasi F, Khoshfetrat AB, Ghaleh HJ. Microstructure and characteristic properties of gelatin/chitosan scaffold prepared by a combined freeze-drying/leaching method. Mater Sci Eng C. 2013;33(7): 3958-3967.
27.    Sarker B, Singh R, Silva R, Roether JA, Kaschta J, Detsch R, Schubert DW, Cicha I, Boccaccini AR. Evaluation of fibroblasts adhesion and proliferation on alginate-gelatin crosslinked hydrogel. PloS One. 2014;9(9):e107952.
28.    Hajian M, Mahmoodi M, Imani R. In vitro assessment of poly (vinyl alcohol) film incorporating aloe vera for potential application as a wound dressing. J Macromol Sci Phys. 2017;56(7):435-450.
29.    Rajati H, Alvandi H, Rahmatabadi SS, Hosseinzadeh L, Arkan E. A nanofiber-hydrogel composite from green synthesized AgNPs embedded to PEBAX/PVA hydrogel and PA/Pistacia atlantica gum nanofiber for wound dressing.  Int J Biol Macromol. 2023;226:1426-1443.
30.    Alvandi H, Rajati H, Naseriyeh T, Rahmatabadi SS, Hosseinzadeh L, Arkan E. Incorporation of Aloe vera and green synthesized ZnO nanoparticles into the chitosan/PVA nanocomposite hydrogel for wound dressing application. Polym Bull. 2024;81(5):4123-4148.
31.    Fayyazbakhsh F, Khayat MJ, Leu MC. 3D-printed gelatin-alginate hydrogel dressings for burn wound healing: A comprehensive study.  Int J Bioprint. 2022;8(4).
32.    Jamroży M, Głąb M, Kudłacik-Kramarczyk S, Drabczyk A, Gajda P, Tyliszczak B. The impact of the Matricaria chamomilla L. Extract, Starch Solution and the Photoinitiator on physiochemical properties of Acrylic Hydrogels. Materials. 2022;15(8):2837.
33.    Sharma A, Mittal A, Puri V, Kumar P, Singh I. Curcumin-loaded, alginate–gelatin composite fibers for wound healing applications. 3 Biotech. 2020;10:1-3.
34.    Boateng J, Burgos-Amador R, Okeke O, Pawar H. Composite alginate and gelatin based bio-polymeric wafers containing silver sulfadiazine for wound healing. Int J Biol Macromol. 2015;79:63-71.
35.    Hamelian M, Zangeneh MM, Amisama A, Varmira K, Veisi H. Green synthesis of silver nanoparticles using Thymus kotschyanus extract and evaluation of their antioxidant, antibacterial and cytotoxic effects. Appl Organomet Chem. 2018;32(9):e4458.
36.    Diniz FR, Maia RC, de Andrade LR, Andrade LN, Vinicius Chaud M, da Silva CF, Corrêa CB, de Albuquerque Junior RL, Pereira da Costa L, Shin SR, Hassan S. Silver nanoparticles-composing alginate/gelatine hydrogel improves wound healing in vivo. Nanomaterials. 2020;10(2):390.
37.    Julaeha E, Puspita WR, Permadi N, Harja A, Nurjanah S, Wahyudi T, Al-Anshori J. Optimization of Citrus aurantifolia peel extract encapsulation in alginate-gelatin hydrogel microbeads for antibacterial wound dressing applications. Carbohydrate Polymer Technologies and Applications. 2024;7:100406.
38.    Khan MU, Razaq SI, Mehboob H, Rehman S, Al-Arjan WS, Amin R. Antibacterial and hemocompatible pH-responsive hydrogel for skin wound healing application: In vitro drug release. Polymers. 2021;13(21):3703.
39.    Ng VW, Chan JM, Sardon H, Ono RJ, García JM, Yang YY, Hedrick JL. Antimicrobial hydrogels: A new weapon in the arsenal against multidrug-resistant infections. Adv Drug Deliv Rev. 2014;78:46-62.
40.    Ahovan ZA, Esmaeili Z, Eftekhari BS, Khosravimelal S, Alehosseini M, Orive G, Dolatshahi-Pirouz A, Chauhan NP, Janmey PA, Hashemi A, Kundu SC. Antibacterial smart hydrogels: New hope for infectious wound management. Mater Today Bio. 2022;17:100499.

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