سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Fayyaz, Talha, Abbas, Ghulam, Ahmed, Hammad, Khatian, Najeeb, Usman, Shumaila, Nisar, Uzair, Ain, Noor, Khurshid, Yamna, Ali, Syed. (1404). Meloxicam mitigated methylglyoxal-induced glycative stress in rats. , 28(5), 647-654. doi: 10.22038/ijbms.2025.83856.18143
Talha Bin Fayyaz; Ghulam Abbas; Hammad Ahmed; Najeeb Khatian; Shumaila Usman; Uzair Nisar; Noor Ul Ain; Yamna Khurshid; Syed Abid Ali. "Meloxicam mitigated methylglyoxal-induced glycative stress in rats". , 28, 5, 1404, 647-654. doi: 10.22038/ijbms.2025.83856.18143
Fayyaz, Talha, Abbas, Ghulam, Ahmed, Hammad, Khatian, Najeeb, Usman, Shumaila, Nisar, Uzair, Ain, Noor, Khurshid, Yamna, Ali, Syed. (1404). 'Meloxicam mitigated methylglyoxal-induced glycative stress in rats', , 28(5), pp. 647-654. doi: 10.22038/ijbms.2025.83856.18143
Fayyaz, Talha, Abbas, Ghulam, Ahmed, Hammad, Khatian, Najeeb, Usman, Shumaila, Nisar, Uzair, Ain, Noor, Khurshid, Yamna, Ali, Syed. Meloxicam mitigated methylglyoxal-induced glycative stress in rats. , 1404; 28(5): 647-654. doi: 10.22038/ijbms.2025.83856.18143

Meloxicam mitigated methylglyoxal-induced glycative stress in rats

Iranian Journal of Basic Medical Sciences
مقاله 12، دوره 28، شماره 5، مرداد 2025، صفحه 647-654    اصل مقاله (738.53 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2025.83856.18143
نویسندگان
Talha Bin Fayyaz1؛ Ghulam Abbas* 1؛ Hammad Ahmed1؛ Najeeb Khatian1؛ Shumaila Usman2؛ Uzair Nisar1؛ Noor Ul Ain3؛ Yamna Khurshid2؛ Syed Abid Ali4
1Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Ziauddin University, Karachi-75600, Pakistan
2Department of Molecular Medicine, Ziauddin University, Karachi-75600, Pakistan
3Department of Pharmacology, Institute of Pharmaceutical Sciences, Jinnah Sindh Medical University, Karachi, Pakistan
4H.E.J. Research Institute of Chemistry, International Center for Chemical & Biological Sciences, University of Karachi, Karachi-75270, Pakistan
چکیده
Objective(s): Glycation is one of the primary underlying processes attributed to senescence and related diseases. No medicine currently targets this harmful manifestation. Drug repurposing is an efficient and cost-effective way of developing drugs. The present study evaluated meloxicam, a clinically used NSAID, for its ability to offer protection against glycative stress.
Materials and Methods: Methylglyoxal (MGO; 17.25 mg/kg) was administered for two weeks to create a rat model of glycative stress. Aminoguanidine (AG; 50 mg/kg) and Meloxicam (MEL; 0.15, 0.3, and 0.6 mg/kg) were used as standard and test agents, respectively. Afterward, the cognitive (Morris Water Maze), liver (LFT), and kidney (Creatinine) functioning were evaluated. The expression of genes of interest (TNF-α, RAGE, BACE, Glyoxalase, and VEGF) were estimated (qPCR) in the liver, brain, and kidney along with histopathology (H&E staining). Carboxymethyllysine (CML) levels in rat plasma were evaluated via ELISA.
Results: MEL treatment has significantly (P<0.05) protected the MGO-induced cognitive (duration in target quadrant, time taken to get to target quadrant, and the frequency of crossings via platform location), hepatic, and renal impairment. The qPCR data revealed that MEL prevented MGO-induced enhancement in the expression of genes of interest. Additionally, the CML levels were significantly (P<0.005) normalized by concomitant administration of MEL. Histopathological examination did not reveal any remarkable outcomes.
Conclusion: MEL has significantly mitigated the rats’ MGO-induced cognitive, liver, and kidney impairments. Hence, it appears to be a potential molecule for repurposing as an antiglycation agent.
کلیدواژه‌ها
Advanced glycation end products؛ Carboxymethyllysine؛ Gene expression؛ Glycation؛ Meloxicam؛ Methylglyoxal
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