سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Raoof, Saeed, Rezaei, Shiva, Zargari, Mehryar, Mirzaei, Masooreh, Hosseinimehr, Seyed Jalal, Karimpour Malekshah, Abbasali, Talebpour Amiri, Fereshteh. (1404). Sinapic acid attenuated nephrotoxicity against Cyclophosphamide in mice model: A histochemical, immunohistochemical and histopathological evaluation. , 28(5), 655-661. doi: 10.22038/ijbms.2025.83903.18155
Saeed Raoof; Shiva Rezaei; Mehryar Zargari; Masooreh Mirzaei; Seyed Jalal Hosseinimehr; Abbasali Karimpour Malekshah; Fereshteh Talebpour Amiri. "Sinapic acid attenuated nephrotoxicity against Cyclophosphamide in mice model: A histochemical, immunohistochemical and histopathological evaluation". , 28, 5, 1404, 655-661. doi: 10.22038/ijbms.2025.83903.18155
Raoof, Saeed, Rezaei, Shiva, Zargari, Mehryar, Mirzaei, Masooreh, Hosseinimehr, Seyed Jalal, Karimpour Malekshah, Abbasali, Talebpour Amiri, Fereshteh. (1404). 'Sinapic acid attenuated nephrotoxicity against Cyclophosphamide in mice model: A histochemical, immunohistochemical and histopathological evaluation', , 28(5), pp. 655-661. doi: 10.22038/ijbms.2025.83903.18155
Raoof, Saeed, Rezaei, Shiva, Zargari, Mehryar, Mirzaei, Masooreh, Hosseinimehr, Seyed Jalal, Karimpour Malekshah, Abbasali, Talebpour Amiri, Fereshteh. Sinapic acid attenuated nephrotoxicity against Cyclophosphamide in mice model: A histochemical, immunohistochemical and histopathological evaluation. , 1404; 28(5): 655-661. doi: 10.22038/ijbms.2025.83903.18155

Sinapic acid attenuated nephrotoxicity against Cyclophosphamide in mice model: A histochemical, immunohistochemical and histopathological evaluation

Iranian Journal of Basic Medical Sciences
مقاله 13، دوره 28، شماره 5، مرداد 2025، صفحه 655-661    اصل مقاله (511.44 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2025.83903.18155
نویسندگان
Saeed Raoof1، 2؛ Shiva Rezaei1، 2؛ Mehryar Zargari3؛ Masooreh Mirzaei1؛ Seyed Jalal Hosseinimehr4؛ Abbasali Karimpour Malekshah1؛ Fereshteh Talebpour Amiri* 1
1Department of Anatomy, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran
2Student Research Committee, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
3Department of Biochemistry, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
4Department of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
چکیده
Objective(s): Cyclophosphamide (CP) is a chemotherapeutic drug used to treat various tumors. It causes nephrotoxicity by producing reactive oxygen species. Sinapic acid (SA) exhibits anti-oxidant, antiapoptotic, and anti-inflammatory activities at low doses as a phenylpropanoid. This study aimed to investigate the protective effects of SA on SP-induced renal injury. 
Materials and Methods: Forty-eight BALB/c mice were randomly divided into control, SA (for seven consecutive days, with two doses of 5 and 10 mg/kg), CP (single dose, 200 mg/kg), and CP + SA (5 and 10 mg/kg). On the 10th day of the study, mice were examined by renal function markers (Urea and Creatinine), oxidative stress markers (MDA and GSH), histopathological, and immunohistochemical assays (caspase-3 and NF-kB kidney). 
Results: MDA levels increased and GSH levels decreased significantly in CP-treated mice. In addition, the histopathological structure of the kidney tissue in CP-treated mice showed significantly severe kidney tissue damage associated with increased urea and creatinine. The administration of SA in CP-treated mice significantly reduced serum urea and creatinine concentrations. In addition, the immunohistochemical staining of caspase- 3 and NF-kB decreased significantly in the CP + SA group compared to CP-treated mice. 
Conclusion: Overall, our study suggests that sinapic acid, a substance with antioxidant, antiapoptotic, and anti-inflammatory properties, can be used as a complementary therapy to protect nephrotoxicity against CP.
کلیدواژه‌ها
Caspase-3؛ Cyclophosphamide؛ Nephrotoxicity؛ NF- kB؛ Oxidative stress؛ Sinapic acid
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