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Şimşek, Hasan, Akaras, Nurhan, Gur, Cihan, Kucukler, Sefa, Ileriturk, Mustafa, Kandemir, Fatih. (1404). Ameliorative effect of morin on diclofenac-induced testicular toxicity in rats: An investigation into different signal pathways. , 28(4), 507-515. doi: 10.22038/ijbms.2025.79735.17270
Hasan Şimşek; Nurhan Akaras; Cihan Gur; Sefa Kucukler; Mustafa Ileriturk; Fatih Mehmet Kandemir. "Ameliorative effect of morin on diclofenac-induced testicular toxicity in rats: An investigation into different signal pathways". , 28, 4, 1404, 507-515. doi: 10.22038/ijbms.2025.79735.17270
Şimşek, Hasan, Akaras, Nurhan, Gur, Cihan, Kucukler, Sefa, Ileriturk, Mustafa, Kandemir, Fatih. (1404). 'Ameliorative effect of morin on diclofenac-induced testicular toxicity in rats: An investigation into different signal pathways', , 28(4), pp. 507-515. doi: 10.22038/ijbms.2025.79735.17270
Şimşek, Hasan, Akaras, Nurhan, Gur, Cihan, Kucukler, Sefa, Ileriturk, Mustafa, Kandemir, Fatih. Ameliorative effect of morin on diclofenac-induced testicular toxicity in rats: An investigation into different signal pathways. , 1404; 28(4): 507-515. doi: 10.22038/ijbms.2025.79735.17270

Ameliorative effect of morin on diclofenac-induced testicular toxicity in rats: An investigation into different signal pathways

Iranian Journal of Basic Medical Sciences
مقاله 13، دوره 28، شماره 4، تیر 2025، صفحه 507-515    اصل مقاله (1.13 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2025.79735.17270
نویسندگان
Hasan Şimşek* 1؛ Nurhan Akaras2؛ Cihan Gur3؛ Sefa Kucukler4؛ Mustafa Ileriturk5؛ Fatih Mehmet Kandemir6
1Department of Physiology, Faculty of Medicine, Aksaray University, Aksaray, Türki̇ye
2Department of Histology and Embryology, Faculty of Medicine, Aksaray University, Aksaray, Türki̇ye
3Department of Medical Laboratory Techniques, Vocational School of Health Services, Atatürk University, Erzurum, Türki̇ye
4Department of Veterinary Biochemistry, Faculty of Veterinary, Atatürk University, Erzurum, Türki̇ye
5Department of Animal Science, Horasan Vocational College, Atatürk University, Erzurum, Türki̇ye
6Department of Medical Biochemistry, Faculty of Medicine, Aksaray University, Aksaray, Türki̇ye
چکیده
Objective(s): Diclofenac (Diclo) is a therapeutic agent used in the treatment of pain and inflammatory diseases, but it is also toxic to the human body. Morin is a flavonoid found naturally in plants and has many biological and pharmacological activities, including anti-inflammatory, anti-oxidant, and anticancer activities. This study aimed to investigate the efficacy of Morin in Diclo-induced testicular toxicity. 
Materials and Methods: Morin (50 mg/kg and 100 mg/kg) was administered orally for five days, while Diclo was administered intraperitoneally at 50 mg/kg on days 4 and 5. Biochemical, molecular, and histological methods were used to investigate oxidative stress, inflammation, apoptosis, and endoplasmic reticulum (ER) stress damage indicators in testicular tissue. 
Results: Morin treatment attenuated Diclo-induced oxidative stress damage by increasing anti-oxidant levels (SOD, CAT, GPx, GSH, Nrf-2, HO-1, and NQO1) and decreasing MDA levels, an indicator of lipid peroxidation. Morin reduced levels of the inflammatory mediators NF-κB protein. Increases in apoptotic Bax and Caspase-3 by Diclo were reduced by Morin, while decreased antiapoptotic Bcl-2 level was increased. Morin reduced Diclo-induced ER stress injury by decreasing ATF-6, PERK, IRE1, GRP-78, and CHOP levels. Also, Diclo decreased COX-2 levels.
Conclusion: Overall, Morin may be an effective treatment of choice for testicular tissue damage associated with Diclo toxicity and may reduce the level of damage.
کلیدواژه‌ها
Apoptosis؛ Diclofenac؛ Endoplasmic reticulum stress؛ Inflammation؛ Morin؛ Oxidative stress
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