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Tian, Chenchen, Yu, Bo, Liu, Yibo, Diao, Zhipeng, Wang, Yue, Zhou, Jianmei. (1404). Apigenin attenuates myocardial ischemia-reperfusion injury through miR-448/SIRT1 axis. , 28(5), 602-611. doi: 10.22038/ijbms.2025.80172.17365
Chenchen Tian; Bo Yu; Yibo Liu; Zhipeng Diao; Yue Wang; Jianmei Zhou. "Apigenin attenuates myocardial ischemia-reperfusion injury through miR-448/SIRT1 axis". , 28, 5, 1404, 602-611. doi: 10.22038/ijbms.2025.80172.17365
Tian, Chenchen, Yu, Bo, Liu, Yibo, Diao, Zhipeng, Wang, Yue, Zhou, Jianmei. (1404). 'Apigenin attenuates myocardial ischemia-reperfusion injury through miR-448/SIRT1 axis', , 28(5), pp. 602-611. doi: 10.22038/ijbms.2025.80172.17365
Tian, Chenchen, Yu, Bo, Liu, Yibo, Diao, Zhipeng, Wang, Yue, Zhou, Jianmei. Apigenin attenuates myocardial ischemia-reperfusion injury through miR-448/SIRT1 axis. , 1404; 28(5): 602-611. doi: 10.22038/ijbms.2025.80172.17365

Apigenin attenuates myocardial ischemia-reperfusion injury through miR-448/SIRT1 axis

Iranian Journal of Basic Medical Sciences
مقاله 7، دوره 28، شماره 5، مرداد 2025، صفحه 602-611    اصل مقاله (736.44 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2025.80172.17365
نویسندگان
Chenchen Tian1؛ Bo Yu2؛ Yibo Liu1؛ Zhipeng Diao1؛ Yue Wang* 1؛ Jianmei Zhou* 3
1School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
2Department of Cardiology, Tianjin Union Medical Center, Nankai University Affiliated Hospital, Tianjin 300121, China
3Department of Cadre Health Care, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China
چکیده
Objective(s): Myocardial ischemia/reperfusion injury (MIRI) is the primary pathological injury following ischemic cardiomyocyte therapy, but there are few effective treatments available for MIRI. Apigenin (API) is an active ingredient of herbal medicine. Our study aims to verify whether API regulates autophagy and apoptosis against MIRI via miR-448/Sirtuin-1 (SIRT1) axis.
Materials and Methods: MTT, SOD, and LDH assays were used to measure cell viability, oxidative stress injury, and cell damage, respectively. RT-qPCR, western blot, and ELISA were used to measure RNA and protein expression levels.
Results: Compared with the control group, cell viability and SOD levels in the cells of the OGD/R group were significantly decreased, LDH release in the cells was significantly increased, the level of miR-448 in the cells was significantly increased, the levels of SIRT1 mRNA and protein in the cells were significantly increased, the expression of LCII/I and Bcl-2 proteins in the cells were significantly down-regulated, and the expression of p62, Bax proteins in the cells and caspase-3 protein in the cell supernatant were significantly up-regulated. Compared with the OGD/R group, the above indicators were significantly reversed in the OGD/R+API group and the OGD/R+miR-448 inhibitor group. Compared to the OGD/R+miR-448 inhibitor group, the above indicators were significantly reversed in the OGD/R+miR-448 inhibitor+EX527 (SIRT1 inhibitor) group. Compared to the OGD/R+API group, the above indicators were significantly reversed in the OGD/R+API+miR-448 mimic group, OGD/R+API+EX527 group, and OGD/R+API+CA-5f (autophagy inhibitor) group.
Conclusion: API regulates autophagy and apoptosis via the miR-448/SIRT1 axis against MIRI.
کلیدواژه‌ها
Apigenin؛ Apoptosis؛ Autophagy؛ MiR-448؛ OGD/R
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