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Wang, Junfeng, Kong, Dalu. (1404). Synergistic effects of cryoablation and GM-CSF in colorectal liver metastases management in tumor-bearing mice. , 28(5), 584-591. doi: 10.22038/ijbms.2025.82910.17980
Junfeng Wang; Dalu Kong. "Synergistic effects of cryoablation and GM-CSF in colorectal liver metastases management in tumor-bearing mice". , 28, 5, 1404, 584-591. doi: 10.22038/ijbms.2025.82910.17980
Wang, Junfeng, Kong, Dalu. (1404). 'Synergistic effects of cryoablation and GM-CSF in colorectal liver metastases management in tumor-bearing mice', , 28(5), pp. 584-591. doi: 10.22038/ijbms.2025.82910.17980
Wang, Junfeng, Kong, Dalu. Synergistic effects of cryoablation and GM-CSF in colorectal liver metastases management in tumor-bearing mice. , 1404; 28(5): 584-591. doi: 10.22038/ijbms.2025.82910.17980

Synergistic effects of cryoablation and GM-CSF in colorectal liver metastases management in tumor-bearing mice

Iranian Journal of Basic Medical Sciences
مقاله 5، دوره 28، شماره 5، مرداد 2025، صفحه 584-591    اصل مقاله (626.28 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2025.82910.17980
نویسندگان
Junfeng Wang1، 2، 3، 4؛ Dalu Kong* 1، 2، 3، 4
1Department of Colorectal Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin, 300060, China
2National Clinical Research Center for Cancer, Tianjin, 300060, China
3Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, China
4Tianjin’s Clinical Research Center for Cancer, Tianjin, 300060, China
چکیده
Objective(s): The use of cryoablation for colorectal liver metastases (CLM) remains limited and controversial. This study aimed to investigate the antitumor immune response following cryoablation combined with granulocyte-macrophage colony-stimulating factor (GM-CSF) treatment in a CLM mouse model.
Materials and Methods: A CLM mouse model was established using BALB/c mice. The tumor-bearing mice were randomly divided into Control group, GM-CSF group, cryoablation group, and cryoablation + GM-CSF group. Tumor size, survival time, dendritic cells (DCs) count, serum cytokine levels (IL-4, IFN-γ), and the Th1/Th2 ratio (IFN-γ/IL-4) were compared among the four groups.
Results: The combination of cryoablation and GM-CSF demonstrated synergistic effects, resulting in the smallest tumor lesion, longest mean survival time, and highest DC count on day 21 post-treatment compared to other groups. Both cryoablation alone and combined with GM-CSF significantly increased serum IFN-γ levels and suppressed IL-4 levels on day 21 compared to pre-treatment levels (P<0.05). Notably, the combination of cryoablation and GM-CSF significantly elevated the Th1/Th2 ratio (P<0.05).
Conclusion: Combining cryoablation with GM-CSF treatment holds promise for CLM management. It exhibits increased DC infiltration within the tumor microenvironment, enhanced immune responses, and prolonged survival in tumor-bearing mice.
کلیدواژه‌ها
Colorectal neoplasms؛ Cryosurgery؛ Cytokines؛ Dendritic cells؛ Granulocyte-macrophage - colony-stimulating factor؛ Immunity؛ Liver neoplasms؛ Tumor microenvironment
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