سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Jalili, Shirin, Mosafer, Jafar, Mousavinezhad, Seyed Amin, Sazgarnia, Ameneh, Mohaghegh, Mohammad Ali, Hoseini, Mehdi. (1404). In vitro and in vivo evaluation of photo-induced antileishmanial activity of indocyanine green-loaded nanomicelles. , 28(4), 498-506. doi: 10.22038/ijbms.2025.82333.17807
Shirin Jalili; Jafar Mosafer; Seyed Amin Mousavinezhad; Ameneh Sazgarnia; Mohammad Ali Mohaghegh; Mehdi Hoseini. "In vitro and in vivo evaluation of photo-induced antileishmanial activity of indocyanine green-loaded nanomicelles". , 28, 4, 1404, 498-506. doi: 10.22038/ijbms.2025.82333.17807
Jalili, Shirin, Mosafer, Jafar, Mousavinezhad, Seyed Amin, Sazgarnia, Ameneh, Mohaghegh, Mohammad Ali, Hoseini, Mehdi. (1404). 'In vitro and in vivo evaluation of photo-induced antileishmanial activity of indocyanine green-loaded nanomicelles', , 28(4), pp. 498-506. doi: 10.22038/ijbms.2025.82333.17807
Jalili, Shirin, Mosafer, Jafar, Mousavinezhad, Seyed Amin, Sazgarnia, Ameneh, Mohaghegh, Mohammad Ali, Hoseini, Mehdi. In vitro and in vivo evaluation of photo-induced antileishmanial activity of indocyanine green-loaded nanomicelles. , 1404; 28(4): 498-506. doi: 10.22038/ijbms.2025.82333.17807

In vitro and in vivo evaluation of photo-induced antileishmanial activity of indocyanine green-loaded nanomicelles

Iranian Journal of Basic Medical Sciences
مقاله 12، دوره 28، شماره 4، تیر 2025، صفحه 498-506    اصل مقاله (417.04 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2025.82333.17807
نویسندگان
Shirin Jalili1؛ Jafar Mosafer2، 3؛ Seyed Amin Mousavinezhad4؛ Ameneh Sazgarnia5؛ Mohammad Ali Mohaghegh3، 6؛ Mehdi Hoseini* 7
1Institute of Police Equipment and Technologies, Policing Sciences and Social Studies Research Institute, Tehran, Iran
2Research Center of Advanced Technologies in Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
3Health Sciences Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
4Research Center for Life & Health Sciences & Biotechnology of the Police, Directorate of Health, Rescue & Treatment, Police Headquarters, Tehran, Iran
5Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
6Department of Laboratory Sciences, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
7Department of Radiology Technology, School of Paramedical Sciences, Torbat Heydarieh University of Medical Sciences, Torbat Heydarieh, Iran
چکیده
Objective(s): Due to its low toxicity and high absorbance in the range of 650 to 900 nm, indocyanine green (ICG) has garnered significant attention for its applications in photodynamic therapy (PDT) and photothermal therapy (PTT). However, its tendency to aggregate in aqueous environments limits its efficacy in both in vitro and in vivo applications. Encapsulating ICG in a biocompatible nanomicelle can improve its aqueous stability and photophysical properties. The present study investigated the synergistic effect of ICG-loaded nanomicelles upon irradiation by an 808-nm laser on Leishmania major (L. major) parasites. 
Materials and Methods: Initially, a nanomicelle comprised ICG was synthesized and characterized. Then, the temperature increase during irradiation and promastigote viability were evaluated in vitro. Subsequently, the prepared samples’ in vitro dark toxicity and phototoxicity were assessed via the MTS assay. Finally, the in vivo antileishmanial efficacy of the ICG-loaded nanomicelles formulation was investigated in BALB/c mice. 
Results: The absorbance of ICG-loaded nanomicelles at 808 nm was more than 2 times greater than Free-ICG. Also, the prepared formulation exhibited a mean diameter of ~25 nm and a zeta potential of -2.3 ± 1 mV. The combination of ICG-loaded nanomicelles and 808 nm laser with a power density of 2.5 W cm−2 led to a significant reduction in the survival rate of promastigotes and lesion size of infected mice compared to control groups. 
Conclusion: The PDT/PTT mediated by ICG-loaded nanomicelles can be considered a promising and efficient therapeutic method for L. major, as it is inexpensive, safe, and easy to implement.
کلیدواژه‌ها
Leishmania major؛ Nanomedicine؛ Photodynamic therapy (PDT)؛ Photosensitizer؛ Photothermal therapy (PTT)
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