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Ezer, Mehmet, Öztürkler, Melek, Yıldız Dalgınlı, Kezban, Atakisi, Emine, Beseren Havadar, Hatice, Atakisi, Onur. (1404). Investigation of the molecular and cellular effects of Shilajit on 5-fluorouracil (5-FU)-induced nephrotoxicity in rats. , 28(5), 565-574. doi: 10.22038/ijbms.2025.80989.17525
Mehmet Ezer; Melek Öztürkler; Kezban Yıldız Dalgınlı; Emine Atakisi; Hatice Beseren Havadar; Onur Atakisi. "Investigation of the molecular and cellular effects of Shilajit on 5-fluorouracil (5-FU)-induced nephrotoxicity in rats". , 28, 5, 1404, 565-574. doi: 10.22038/ijbms.2025.80989.17525
Ezer, Mehmet, Öztürkler, Melek, Yıldız Dalgınlı, Kezban, Atakisi, Emine, Beseren Havadar, Hatice, Atakisi, Onur. (1404). 'Investigation of the molecular and cellular effects of Shilajit on 5-fluorouracil (5-FU)-induced nephrotoxicity in rats', , 28(5), pp. 565-574. doi: 10.22038/ijbms.2025.80989.17525
Ezer, Mehmet, Öztürkler, Melek, Yıldız Dalgınlı, Kezban, Atakisi, Emine, Beseren Havadar, Hatice, Atakisi, Onur. Investigation of the molecular and cellular effects of Shilajit on 5-fluorouracil (5-FU)-induced nephrotoxicity in rats. , 1404; 28(5): 565-574. doi: 10.22038/ijbms.2025.80989.17525

Investigation of the molecular and cellular effects of Shilajit on 5-fluorouracil (5-FU)-induced nephrotoxicity in rats

Iranian Journal of Basic Medical Sciences
مقاله 3، دوره 28، شماره 5، مرداد 2025، صفحه 565-574    اصل مقاله (619.05 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2025.80989.17525
نویسندگان
Mehmet Ezer* 1؛ Melek Öztürkler2؛ Kezban Yıldız Dalgınlı3؛ Emine Atakisi4؛ Hatice Beseren Havadar5؛ Onur Atakisi2
1Kafkas University Medical School, Department of Urology, Kars, Türkiye
2Kafkas University Department of Chemistry, Faculty Science and Letter, Kars, Türkiye
3Department of Chemistry and Chemical Processing Technologies, Kars Vocational High School Kafkas University, Kars, Türkiye
4Kafkas University, Faculty of Veterinary Medicine, Department of Biochemistry, Kars, Türkiye
5Kafkas University Health Research and Application Hospital, Pathology Laboratory, Kars, Türkiye
چکیده
Objective(s): This study aimed to evaluate the protective effects of shilajit on 5-fluorouracil (5-FU)-induced nephrotoxicity in a rat model.
Materials and Methods: Twenty male Sprague Dawley rats were divided into four groups: Control, 5-FU, shilajit, and 5-FU + shilajit. 5-FU was administered intraperitoneally at 200 mg/kg, while shilajit was given orally at 200 mg/kg. Kidney tissues were analyzed for oxidative stress markers, protein expression (SIRT2, SIRT3, β-catenin, E-cadherin, and caspase-3), and histopathological changes.
Results: 5-FU significantly increased oxidative stress parameters and kidney damage markers. Shilajit co-administration with 5-FU reduced oxidative stress and increased anti-oxidant status and SIRT2, SIRT3, and cell adhesion protein expression. Histopathological evaluation showed reduced renal damage in the shilajit + 5-FU group compared to the 5-FU group.
Conclusion: Shilajit exhibited significant protective effects against 5-FU-induced nephrotoxicity, improving oxidative parameters, protein expression, and kidney histopathology. Further studies are needed to elucidate its molecular mechanism and therapeutic potential.
کلیدواژه‌ها
Caspase 3؛ Fluorouracil؛ Resins؛ Plant؛ Nephrotoxicity؛ Sirtuin 2؛ Sirtuin 3
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