Comparing the effect of plasma therapy with estradiol valerate in motor and cognitive behavioral disorders in ovariectomized old rats: Behavioral, biochemical, and protein expression | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 12، دوره 28، شماره 3، خرداد 2025، صفحه 366-375 اصل مقاله (742.95 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2024.81345.17608 | ||
| نویسندگان | ||
| Maryam Khajvand-Abedini1؛ Mohammad Mohammadi2؛ Parisa Habibi* 3؛ Zahra Shahabi4؛ Siamak Shahidi4؛ Naser Ahmadiasl5؛ Mohammad Reza Alipour4؛ Mahdi Ramezani6؛ Alireza Komaki4 | ||
| 1Department of Clinical Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran | ||
| 22 Department of Medical Laboratory Science, Faculty of Medicine, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran | ||
| 3Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran | ||
| 4Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran | ||
| 5Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 6Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran | ||
| چکیده | ||
| Objective(s): This study investigated the effects of young plasma therapy (YPT) compared to estrogen therapy (E2T) on motor and cognitive impairments in aged ovariectomized (OVX) rats. Materials and Methods: Sixty female Wistar rats were divided as follows: 1). 2-3 months control young group. Five 22-24 months old groups: 1) Control, 2) Sham, 3) OVX, 4) OVX.E2, and 5) OVX.YP. Young plasma (1 ml plasma, through the tail vein, 3 days weekly for 4 weeks) and E2 (30 mg/kg, SC, 5 days weekly for 4 weeks) were administrated to OVX rats. The open field, elevated plus maze, and Barne’s maze were used to assess the behaviors. Then, miR-134 and miR-124 (RT- RCR), SIRT1, CREB, and BDNF (western blot), and anti-oxidants/oxidants markers (Photometry) levels were assessed in the rat’s hippocampal tissues. Results: OVX caused up-regulated hippocampal miR-134 and miR-124 expression levels (P<0.001) while down-regulated SIRT1, CREB, and BDNF protein expressions (P<0.001). Also, ovariectomy Increased TOS, OSI, and MDA (P<0.001) while decreasing TAC (P<0.001) compared to sham. Treatment with both E2T and YPT significantly improved all oxidative stress indexes (P<0.0.001) and increased p-CREB, BDNF, and SIRT1 protein levels (P<0.05, P<0.01) while decreasing the expression of miR-134 and miR-124 (P<0.001). Conclusion: YPT is a non-pharmacological therapeutic as much as or more than E-2T, which can exhibit anti-oxidative and anti-inflammatory potential in the hippocampal tissue and improve cognitive deficits in aged OVX rats without unknown side effects. | ||
| کلیدواژهها | ||
| Cognition؛ Estradiol valerate؛ Motor؛ Ovariectomy؛ Protein expression؛ Young plasma | ||
| مراجع | ||
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