سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Mahheidari, Naimeh, Alizadeh, Morteza, Kamalabadi farahani, Mohammad, Arabpour, Zohreh, Rezaei kolarijani, Nariman, Djalilian, Ali R., Salehi, Majid. (1403). Regeneration of the skin wound by two different crosslinkers: In vitro and in vivo studies. , 28(2), 194-208. doi: 10.22038/ijbms.2024.80137.17361
Naimeh Mahheidari; Morteza Alizadeh; Mohammad Kamalabadi farahani; Zohreh Arabpour; Nariman Rezaei kolarijani; Ali R. Djalilian; Majid Salehi. "Regeneration of the skin wound by two different crosslinkers: In vitro and in vivo studies". , 28, 2, 1403, 194-208. doi: 10.22038/ijbms.2024.80137.17361
Mahheidari, Naimeh, Alizadeh, Morteza, Kamalabadi farahani, Mohammad, Arabpour, Zohreh, Rezaei kolarijani, Nariman, Djalilian, Ali R., Salehi, Majid. (1403). 'Regeneration of the skin wound by two different crosslinkers: In vitro and in vivo studies', , 28(2), pp. 194-208. doi: 10.22038/ijbms.2024.80137.17361
Mahheidari, Naimeh, Alizadeh, Morteza, Kamalabadi farahani, Mohammad, Arabpour, Zohreh, Rezaei kolarijani, Nariman, Djalilian, Ali R., Salehi, Majid. Regeneration of the skin wound by two different crosslinkers: In vitro and in vivo studies. , 1403; 28(2): 194-208. doi: 10.22038/ijbms.2024.80137.17361

Regeneration of the skin wound by two different crosslinkers: In vitro and in vivo studies

Iranian Journal of Basic Medical Sciences
مقاله 7، دوره 28، شماره 2، اردیبهشت 2025، صفحه 194-208    اصل مقاله (967.27 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2024.80137.17361
نویسندگان
Naimeh Mahheidari1؛ Morteza Alizadeh2؛ Mohammad Kamalabadi farahani3؛ Zohreh Arabpour4؛ Nariman Rezaei kolarijani1؛ Ali R. Djalilian4؛ Majid Salehi* 3، 5
1Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
2Department of Tissue Engineering and Biomaterials, School of Advanced Medical Sciences and Technologies, Hamadan University of Medical Sciences, Hamadan, Iran
3Tissue Engineering and stem cells research center, Shahroud University of Medical Sciences, Shahroud, Iran
4Department of Ophthalmology and Visual Sciences, University of Illinois, Chicago, IL 60612, USA
5Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
چکیده
Objective(s): For designing a suitable hydrogel, two crosslinked Alginate/ Carboxymethyl cellulose (Alg/CMC) hydrogel, using calcium chloride (Ca2+) and glutaraldehyde (GA) as crosslinking agents were synthesized and compared.
Materials and Methods: All samples were characterized by Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Blood compatibility (BC), Blood clotting index (BCI), weight loss (WL), water absorption (WA), pH, and Electrochemical Impedance Spectroscopy (EIS). Cell viability and cell migration were investigated using the MTT assay and the wound scratch test, respectively. Besides, the wound healing potential of prepared hydrogels was evaluated on the rat models with full-thickness skin excision. To further investigation, TGF β1, IGF-I, COL1, ACT-A (alfa-SMA), and GAPDH expression levels were also reported by RT-PCR. 
Results: Water absorption and weight loss properties were compared between different crosslinker agents, and the most nontoxic crosslinker concentration was determined. We have shown that GA (20 µl/ml) and Ca2+ (50 or 75 mM) enhanced the physical stability of Alg-CMC hydrogel, and they are nontoxic and suitable crosslinkers for wound dressing applications. Although in vivo assessments indicated that the GA (20 µl/ml) had a cytotoxic effect on tissue repair, Ca2+ (75 mM) boosted the wound healing process. Further, RT-PCR results revealed that TGF β1, IGF-I, COL1, ACT-A (alfa-SMA), and GAPDH expression levels were increased in GA (20 µl/ml). Moreover, this trend is the opposite in the Ca2+ (75 mM) treatment groups.
Conclusion: This research shows that Ca2+ (75 mM) boosts tissue regeneration and wound healing process.
کلیدواژه‌ها
Alginate؛ CaCl2؛ Carboxymethyl cellulose؛ Glutaraldehyde؛ Tissue engineering
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