Effect of thymoquinone on vitamin D metabolism in glucocorticoid-induced insulin resistance | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 3، دوره 28، شماره 3، خرداد 2025، صفحه 292-298 اصل مقاله (354.22 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2024.81932.17725 | ||
| نویسندگان | ||
| Hazel Berna Göktuğ1؛ Semiha Dede* 2 | ||
| 1Van Yuzuncu Yil University, Institute of Health Sciences, Van, Turkiye | ||
| 2Van Yuzuncu Yil University, Faculty of Veterinary Medicine, Biochemistry Department, Van, Turkiye | ||
| چکیده | ||
| Objective(s): The key ingredient in Nigella sativa, thymoquinone (TQ), has several beneficial (antioxidant and anti-inflammatory) properties. This study aimed to investigate the vitamin D metabolism in insulin resistance and the effects of TQ. Materials and Methods: Male Wistar albino rats were used. TQ was administered as a therapy, and prophylaxis and treatment with metformin were set up for the groups in which insulin resistance had been developed. The gene groups implicated in vitamin D metabolism underwent RT-PCR gene expression analysis and western blot protein analysis. Results: The analysis shows that the application of TQ reduced HOMA-IR (a sign of insulin resistance). The expression of the VDR gene may be responsible for TQ's effect on treating insulin resistance. Conclusion: It has been demonstrated that using TQ for therapeutic and preventive reasons is advantageous for improving insulin resistance metrics. Serum vitamin D level was also found to be impacted, which was found to be directly related to the expression of several genes involved in vitamin D metabolism in the liver. However, some of these genes were found to be relatively ineffective in the present study. | ||
| کلیدواژهها | ||
| Glucocorticoid؛ Insulin resistance؛ Metabolism؛ Thymoquinone؛ Vitamin D | ||
| مراجع | ||
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