سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Mehrparvar Tajoddini, Malihe, Gheybi, Elaheh, Rostami, Mehdi, Mousavi, Seyed Hadi, Hashemy, Seyed Isaac, Rashidi, Roghayeh, Soukhtanloo, Mohammad. (1404). Neuroprotective effects of hesperidin and auraptene on 6-hydroxydopamine-induced neurodegeneration in SH-SY5Y cells. , 15(3), 1082-1090. doi: 10.22038/ajp.2024.25214
Malihe Mehrparvar Tajoddini; Elaheh Gheybi; Mehdi Rostami; Seyed Hadi Mousavi; Seyed Isaac Hashemy; Roghayeh Rashidi; Mohammad Soukhtanloo. "Neuroprotective effects of hesperidin and auraptene on 6-hydroxydopamine-induced neurodegeneration in SH-SY5Y cells". , 15, 3, 1404, 1082-1090. doi: 10.22038/ajp.2024.25214
Mehrparvar Tajoddini, Malihe, Gheybi, Elaheh, Rostami, Mehdi, Mousavi, Seyed Hadi, Hashemy, Seyed Isaac, Rashidi, Roghayeh, Soukhtanloo, Mohammad. (1404). 'Neuroprotective effects of hesperidin and auraptene on 6-hydroxydopamine-induced neurodegeneration in SH-SY5Y cells', , 15(3), pp. 1082-1090. doi: 10.22038/ajp.2024.25214
Mehrparvar Tajoddini, Malihe, Gheybi, Elaheh, Rostami, Mehdi, Mousavi, Seyed Hadi, Hashemy, Seyed Isaac, Rashidi, Roghayeh, Soukhtanloo, Mohammad. Neuroprotective effects of hesperidin and auraptene on 6-hydroxydopamine-induced neurodegeneration in SH-SY5Y cells. , 1404; 15(3): 1082-1090. doi: 10.22038/ajp.2024.25214

Neuroprotective effects of hesperidin and auraptene on 6-hydroxydopamine-induced neurodegeneration in SH-SY5Y cells

Avicenna Journal of Phytomedicine
دوره 15، شماره 3، مرداد و شهریور 2025، صفحه 1082-1090    اصل مقاله (855.97 K)
نوع مقاله: Short communication
شناسه دیجیتال (DOI): 10.22038/ajp.2024.25214
نویسندگان
Malihe Mehrparvar Tajoddini1؛ Elaheh Gheybi1؛ Mehdi Rostami1؛ Seyed Hadi Mousavi2؛ Seyed Isaac Hashemy1؛ Roghayeh Rashidi1؛ Mohammad Soukhtanloo* 1
1Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
2Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
چکیده
Objective: Destruction of dopaminergic neurons causes diseases. Various compounds with neuroprotective and antioxidant properties have been identified, including Hesperidin (HES) and Auraptene (AUR). We aimed in this study to evaluate the in vitro protective effects of these compounds in SH-SY5Y neuroblastoma cell line against the induced neurotoxicity of 6-hydroxydopamine (6-OHDA).
Materials and Methods: The MTT test to assess cell viability was used. Flow cytometry was conducted for the cell cycle analysis using propidium iodide (PI) stain. The intracellular production of reactive oxygen species (ROS) was assessed using 2, 7′-dichlorofluorescein diacetate (DCFDA) probe and fluorimetry.
Results: Following 6-OHDA treatment, cell viability decreased, and G2/M arrest and ROS levels increased. Our intervention demonstrated that only HES has neuroprotective effects against 6-OHDA-induced toxicity.
Conclusion: HES protects SH-SY5Y cells against 6-OHDA-induced neural damage via inhibiting G2/M arrest, reducing the amount of ROS, and increasing cell viability. However, the different effects and more precise mechanisms are still unknown, and requires new research on animal and human models.
کلیدواژه‌ها
Hesperidin Auraptene ROS 6؛ hydroxydopamine SH؛ SY5Y cells
آمار
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