سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Anbiaee, Najmeh, Bahreyni Toossi, Mohammad Taghi, Azimian, Hosein, Sahebnasagh, Zoha, Abdollahi Dehkordi, Sepideh, Kianmehr, Majid, soudmand salarabadi, samaneh, Najafi Amiri, Maryam. (1403). Anti-Apoptosis Effects of Green Tea in Diagnostic and Therapeutic Doses of Ionizing Radiation. , 21(4), 222-227. doi: 10.22038/ijmp.2024.74945.2330
Najmeh Anbiaee; Mohammad Taghi Bahreyni Toossi; Hosein Azimian; Zoha Sahebnasagh; Sepideh Abdollahi Dehkordi; Majid Kianmehr; samaneh soudmand salarabadi; Maryam Najafi Amiri. "Anti-Apoptosis Effects of Green Tea in Diagnostic and Therapeutic Doses of Ionizing Radiation". , 21, 4, 1403, 222-227. doi: 10.22038/ijmp.2024.74945.2330
Anbiaee, Najmeh, Bahreyni Toossi, Mohammad Taghi, Azimian, Hosein, Sahebnasagh, Zoha, Abdollahi Dehkordi, Sepideh, Kianmehr, Majid, soudmand salarabadi, samaneh, Najafi Amiri, Maryam. (1403). 'Anti-Apoptosis Effects of Green Tea in Diagnostic and Therapeutic Doses of Ionizing Radiation', , 21(4), pp. 222-227. doi: 10.22038/ijmp.2024.74945.2330
Anbiaee, Najmeh, Bahreyni Toossi, Mohammad Taghi, Azimian, Hosein, Sahebnasagh, Zoha, Abdollahi Dehkordi, Sepideh, Kianmehr, Majid, soudmand salarabadi, samaneh, Najafi Amiri, Maryam. Anti-Apoptosis Effects of Green Tea in Diagnostic and Therapeutic Doses of Ionizing Radiation. , 1403; 21(4): 222-227. doi: 10.22038/ijmp.2024.74945.2330

Anti-Apoptosis Effects of Green Tea in Diagnostic and Therapeutic Doses of Ionizing Radiation

Iranian Journal of Medical Physics
دوره 21، شماره 4، مهر و آبان 2024، صفحه 222-227    اصل مقاله (1.31 M)
نوع مقاله: Original Paper
شناسه دیجیتال (DOI): 10.22038/ijmp.2024.74945.2330
نویسندگان
Najmeh Anbiaee1؛ Mohammad Taghi Bahreyni Toossi2؛ Hosein Azimian3؛ Zoha Sahebnasagh1؛ Sepideh Abdollahi Dehkordi4؛ Majid Kianmehr5؛ samaneh soudmand salarabadi6؛ Maryam Najafi Amiri* 3
1Department of Oral and Maxillofacial Radiology, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.
2Medical Physics Department, Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
3Department of Medical Physics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4Department of Medical Physics, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
5Esfarayen Faculty of Medical Sciences, Esfarayen, Iran.
6Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
چکیده
Introduction: Patients who receive ionizing radiation for diagnosis or treatment may suffer side effects in normal tissues. Radioprotective agents have the potential to decrease DNA damage and free radicals produced by ionizing radiation. Green tea is a natural product from the Camellia sinensis plant and have been shown to have antioxidant and radioprotective properties. The aim of the present study is assessing the protective effect of green tea on lymphocytes affected by radiation injury.
Material and Methods: Blood samples was obtained from four adults healthy human and lymphocytes were extracted. Afterward, lymphocytes were exposed to X-radiation by OPG, CBCT, CT and radiotherapy instruments. The protective effects of the green tea polyphenol on Bcl-2 and Bax genes expression levels were evaluated in cultured lymphocytes obtained from treated cells (24 h and 1 h before and 1 h after exposure to ionizing radiation) and non-treated cells (Receiving X-radiation and non-receiving green tea).
Results: The results of this investigation demonstrated Bax/Bcl-2 ratio, as an index of the radiation sensitivity, was significantly decreased in groups which had been received dose of 2 Gy in treated cells groups of 1 h before and 1 h after X-radiation (p<0.05).
Conclusion: The results indicated different effects of green tea on low and high doses of X-radiation. Most protective effects of green tea as an apoptosis inhibitor were observed in high doses of ionizing radiation. Consequently, green tea may be useful as an adjunct to radiotherapy and medical imaging to diminish apoptosis and side effects in normal tissues.
کلیدواژه‌ها
Apoptosis؛ Gene Expression؛ Radiotherapy؛ Diagnostic Techniques؛ Polyphenol؛ Green Tea
مراجع
  1. Gong L, Zhang Y, Liu C, Zhang M, Han S. Application of radiosensitizers in cancer radiotherapy. International journal of nanomedicine. 2021:1083-102.
  2. Toossi MTB, Amiri MN, Sankian M, Azimian H, Abdollahi S, Khademi S. INF/IL-4 increases after the low doses of gamma radiation in BALB/c spleen lymphocytes. Iranian Journal of Medical Physics/Majallah-I Fīzīk-I Pizishkī-i Īrān. 2019;16(4).
  3. Bahreyni-Toossi M-T, Sankian M, Azimian H, Najafi-Amiri M, Khademi S, Abdollahi-Dehkordi S, et al. In vivo study of interferon-γ, transforming growth factor-β, and interleukin-4 gene expression induced by radioadaptive response. Journal of Cancer Research and Therapeutics. 2021;17(2):537-42.
  4. Haddadi G, Alimoradi H, Fardid R, Zare T, Jorat MV. Investigation of erythema, radiation dose, and radiation-induced apoptosis in the peripheral blood lymphocytes of patients treated with radiofrequency catheter ablation. Iranian Journal of Medical Physics. 2021;18(1):15-22.
  5. Huang H, Ullah F, Zhou D-X, Yi M, Zhao Y. Mechanisms of ROS regulation of plant development and stress responses. Frontiers in plant science. 2019;10:800.
  6. Chatzipapas KP, Papadimitroulas P, Emfietzoglou D, Kalospyros SA, Hada M, Georgakilas AG, et al. Ionizing radiation and complex DNA damage: quantifying the radiobiological damage using monte carlo simulations. Cancers. 2020;12(4):799.
  7. Suetens A, Konings K, Moreels M, Quintens R, Verslegers M, Soors E, et al. Higher initial DNA damage and persistent cell cycle arrest after carbon ion irradiation compared to X-irradiation in prostate and colon cancer cells. Frontiers in oncology. 2016;6:87.
  8. Borràs-Fresneda M, Barquinero J-F, Gomolka M, Hornhardt S, Rössler U, Armengol G, et al. Differences in DNA repair capacity, cell death and transcriptional response after irradiation between a radiosensitive and a radioresistant cell line. Scientific reports. 2016;6(1):1-11.
  9. Golden EB, Apetoh L, editors. Radiotherapy and immunogenic cell death. Seminars in radiation oncology; 2015: Elsevier.
  10. Dong J, Zhang T, Ren Y, Wang Z, Ling CC, He F, et al. Inhibiting DNA-PKcs in a non-homologous end-joining pathway in response to DNA double-strand breaks. Oncotarget. 2017;8(14):22662.
  11. Kiraz Y, Adan A, Kartal Yandim M, Baran Y. Major apoptotic mechanisms and genes involved in apoptosis. Tumor Biology. 2016;37(7):8471-86.
  12. Tohidi F-Z, Sadr-Nabavi A, Haghir H, Fardid R, Rafatpanah H, Azimian H, et al. Long-term exposure to electromagnetic radiation from mobile phones can cause considerable changes in the balance of Bax/Bcl2 mRNA expression in the hippocampus of mice. Electromagnetic Biology and Medicine. 2021;40(1):131-7.
  13. D'Orsi B, Mateyka J, Prehn JH. Control of mitochondrial physiology and cell death by the Bcl-2 family proteins Bax and Bok. Neurochemistry International. 2017;109:162-70.
  14. Islamian JP, Mehrali H. Lycopene as a carotenoid provides radioprotectant and antioxidant effects by quenching radiation-induced free radical singlet oxygen: An overview. Cell Journal (Yakhteh). 2015;16(4):386.
  15. Gondoin A, Grussu D, Stewart D, McDougall GJ. White and green tea polyphenols inhibit pancreatic lipase in vitro. Food Research International. 2010;43(5):1537-44.
  16. Qi F, Shen P, Hu R, Xue T, Jiang X, Qin L, et al. Carotenoids and lipid production from Rhodosporidium toruloides cultured in tea waste hydrolysate. Biotechnology for biofuels. 2020;13(1):1-12.
  17. Nagle DG, Ferreira D, Zhou Y-D. Epigallocatechin-3-gallate (EGCG): chemical and biomedical perspectives. Phytochemistry. 2006;67(17):1849-55.
  18. Lecumberri E, Dupertuis YM, Miralbell R, Pichard C. Green tea polyphenol epigallocatechin-3-gallate (EGCG) as adjuvant in cancer therapy. Clinical Nutrition. 2013;32(6):894-903.
  19. Ishtiaque S, Naz S, Soomro N, Khan K, Siddiqui R. Antioxidant activity and total phenolics content of extracts from Murraya koenigii (curry leaves), Laurus nobilis (bay leaves), and Camellia sinensis (tea). Quaid-E-Awam University Research Journal of Engineering, Science & Technology. 2015;14(2):20-5.
  20. Mohseni M, Mihandoost E, Shirazi A, Sepehrizadeh Z, Bazzaz JT, Ghazi-khansari M. Melatonin may play a role in modulation of bax and bcl-2 expression levels to protect rat peripheral blood lymphocytes from gamma irradiation-induced apoptosis. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 2012;738:19-27.
  21. Cornwall S, Cull G, Joske D, Ghassemifar R. Green tea polyphenol “epigallocatechin-3-gallate”, differentially induces apoptosis in CLL B-and T-Cells but not in healthy B-and T-Cells in a dose dependant manner. Leukemia Research. 2016;51:56-61.
  22. Eroğlu HE. The cytogenetic effects of black tea and green tea on cultured human lymphocytes. Brazilian Archives Of Biology And Technology. 2011;54:1159-66.
  23. Ghorbani Z, Fardid R. Effects of Low-dose Gamma Radiation on Expression of Apoptotic Genes in Rat Peripheral Blood Lymphocyte. Journal of Biomedical Physics & Engineering. 2021;11(6):693.
  24. Mirzaghavami PS, Khoei S, Khoee S, Shirvalilou S, Mahdavi SR, Mahabadi VP. Radio-sensitivity enhancement in HT29 cells through magnetic hyperthermia in combination with targeted nano-carrier of 5-Flourouracil. Materials Science and Engineering: C. 2021;124:112043.
  25. Azimian H, Bahreyni-Toossi MT, Rezaei AR, Rafatpanah H, Hamzehloei T, Fardid R. Up-regulation of Bcl-2 expression in cultured human lymphocytes after exposure to low doses of gamma radiation. Journal of Medical Physics/Association of Medical Physicists of India. 2015;40(1):38.
  26. Azimian H, Dayyani M, Toossi MTB, Mahmoudi M. Bax/Bcl-2 expression ratio in prediction of response to breast cancer radiotherapy. Iranian journal of basic medical sciences. 2018;21(3):325.
  27. Sudprasert W, Navasumrit P, Ruchirawat M. Effects of low-dose gamma radiation on DNA damage, chromosomal aberration and expression of repair genes in human blood cells. International journal of hygiene and environmental health. 2006;209(6):503-11.
  28. Wang L-L, Hu R-C, Dai A-G, Tan S-X, Xu M, Kong C-C, et al. CHOP overexpression sensitizes human non-small cell lung cancer cells to cisplatin treatment by Bcl-2/JNK pathway. American Journal of Translational Research. 2021;13(6):6279.
  29. Alamro AA, Al-Malky MM, Ansari MG, Amer OE, Alnaami AM, Hussain SD, et al. The effects of melatonin and vitamin D3 on the gene expression of BCl-2 and BAX in MCF-7 breast cancer cell line. Journal of King Saud University-Science. 2021;33(2):101287.
  30. Matsuno Y, Atsumi Y, Alauddin M, Rana M, Fujimori H, Hyodo M, et al. Resveratrol and its Related polyphenols contribute to the Maintenance of Genome Stability. Scientific reports. 2020;10(1):1-10.
  31. Davari H, Haddad F, Moghimi A, Rahimi MF, Ghavamnasiri MR. Study of radioprotective effect of green tea against gamma irradiation using micronucleus assay on binucleated human lymphocytes. Iranian Journal of Basic Medical Sciences. 2012;15(5):1026.
  32. Kondo H, Park S-H, Watanabe K, Yamamoto Y, Akashi M. Polyphenol (−)-epigallocatechin gallate inhibits apoptosis induced by irradiation in human HaCaT keratinocytes. Biochemical and biophysical research communications. 2004;316(1):59-64.
  33. Kim M, Murakami A, Miyamoto S, Tanaka T, Ohigashi H. The modifying effects of green tea polyphenols on acute colitis and inflammation‐associated colon carcinogenesis in male ICR mice. Biofactors. 2010;36(1):43-51.
  34. Cheng Z, Zhang Z, Han Y, Wang J, Wang Y, Chen X, et al. A review on anti-cancer effect of green tea catechins. Journal of Functional Foods. 2020;74:104172.
آمار
تعداد مشاهده مقاله: 206
تعداد دریافت فایل اصل مقاله: 176