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Li, Yunxia, Bai, Yunxin, Tang, Shiyu, Sun, Ye, Wang, Zhe, Yang, Biao, Liu, Guangyan. (1403). Effect of the S100A9/AMPK pathway on PM2.5-mediated mouse lung injury. , 28(1), 121-129. doi: 10.22038/ijbms.2024.80242.17374
Yunxia Li; Yunxin Bai; Shiyu Tang; Ye Sun; Zhe Wang; Biao Yang; Guangyan Liu. "Effect of the S100A9/AMPK pathway on PM2.5-mediated mouse lung injury". , 28, 1, 1403, 121-129. doi: 10.22038/ijbms.2024.80242.17374
Li, Yunxia, Bai, Yunxin, Tang, Shiyu, Sun, Ye, Wang, Zhe, Yang, Biao, Liu, Guangyan. (1403). 'Effect of the S100A9/AMPK pathway on PM2.5-mediated mouse lung injury', , 28(1), pp. 121-129. doi: 10.22038/ijbms.2024.80242.17374
Li, Yunxia, Bai, Yunxin, Tang, Shiyu, Sun, Ye, Wang, Zhe, Yang, Biao, Liu, Guangyan. Effect of the S100A9/AMPK pathway on PM2.5-mediated mouse lung injury. , 1403; 28(1): 121-129. doi: 10.22038/ijbms.2024.80242.17374

Effect of the S100A9/AMPK pathway on PM2.5-mediated mouse lung injury

Iranian Journal of Basic Medical Sciences
مقاله 15، دوره 28، شماره 1، فروردین 2025، صفحه 121-129    اصل مقاله (567.72 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2024.80242.17374
نویسندگان
Yunxia Li1؛ Yunxin Bai2؛ Shiyu Tang3؛ Ye Sun2؛ Zhe Wang4، 5؛ Biao Yang* 2؛ Guangyan Liu* 6
1Department of Respiratory and Critical Care Medicine, The Fourth People’s Hospital of Shenyang, Shenyang 110000, China
2Department of Pathogen Biology, Shenyang Medical College, Shenyang. No. 146, Huanghe North Street, Shenyang, People’s Republic of China
3Department of Pathophysiology, Shenyang Medical College, Shenyang. No. 146, Huanghe North Street, Shenyang, China
4Department of Medical Oncology, Affiliated Zhongshan Hospital of Dalian University, Dalian, People’s Republic of China
5The Key Laboratory of Biomarker High Throughput Screening and Target Translation of Breast and Gastrointestinal Tumor, Dalian University, Dalian, People’s Republic of China
6Graduate school, Shenyang Medical College, Shenyang. No. 146, Huanghe North Street, Shenyang, People’s Republic of China
چکیده
Objective(s): Particulate matter 2.5 (PM2.5), particles with an aerodynamic diameter less than 2.5 µm, affect lung function and increase respiratory disease incidence and mortality rate. The molecular mechanism of lung injury and epithelial damage after PM2.5 exposure is not completely clear.
Materials and Methods: Mouth-nose exposure of mice was performed with PM2.5 or neutral saline. In vitro experiments were conducted to investigate the role of the S100A9/AMPK pathway in PM2.5-mediated lung injury.
Results: PM2.5 exposure in mice caused lung epithelial damage, alveolar wall thickening, and alveolar wall structure destruction. The 16S rRNA sequencing results suggested that the microecology structure of lung tissue was altered after PM2.5 exposure. Proteomic sequencing was performed to explore the underlying mechanism, and 71 differentially expressed proteins were identified. KEGG database analysis of the up-regulated differential proteins revealed regulatory networks, including fat digestion and absorption, the AMPK signaling pathway, and the PPAR signaling pathway. Moreover, PM2.5 exposure in mice increased the level of S100A9 and ROS, leading to reduction of the ATP level. To achieve a sufficient energy supply by increasing fatty acid transfer and oxidation, activated AMPK up-regulates CD36 and CPT1, which leads to mitochondrial damage of PM2.5-exposed cells and injury or death of lung epithelial cells. siRNA-S100A9 and AMPK inhibitors significantly reduced the occurrence of cell damage.
Conclusion: These results may help to clarify biomarkers and specific mechanisms of lung tissue injury induced by PM2.5 exposure.
کلیدواژه‌ها
AMPK ATP؛ Lung injury؛ PM2.5؛ ROS؛ S100A9
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