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Rezaeinasab, Hamed, Habibi, Abdolhamid, Rezaei, Ramin, Basereh, Aref, Yurista, Salva, Khoramipour, Kayvan. (1403). Endurance training and pyruvate dehydrogenase kinase 4 (PDK4) inhibition combination is superior to each one alone in attenuating hyperketonemia/ketoacidosis in diabetic rats. , 28(1), 80-86. doi: 10.22038/ijbms.2024.79864.17305
Hamed Rezaeinasab; Abdolhamid Habibi; Ramin Rezaei; Aref Basereh; Salva Reverentia Yurista; Kayvan Khoramipour. "Endurance training and pyruvate dehydrogenase kinase 4 (PDK4) inhibition combination is superior to each one alone in attenuating hyperketonemia/ketoacidosis in diabetic rats". , 28, 1, 1403, 80-86. doi: 10.22038/ijbms.2024.79864.17305
Rezaeinasab, Hamed, Habibi, Abdolhamid, Rezaei, Ramin, Basereh, Aref, Yurista, Salva, Khoramipour, Kayvan. (1403). 'Endurance training and pyruvate dehydrogenase kinase 4 (PDK4) inhibition combination is superior to each one alone in attenuating hyperketonemia/ketoacidosis in diabetic rats', , 28(1), pp. 80-86. doi: 10.22038/ijbms.2024.79864.17305
Rezaeinasab, Hamed, Habibi, Abdolhamid, Rezaei, Ramin, Basereh, Aref, Yurista, Salva, Khoramipour, Kayvan. Endurance training and pyruvate dehydrogenase kinase 4 (PDK4) inhibition combination is superior to each one alone in attenuating hyperketonemia/ketoacidosis in diabetic rats. , 1403; 28(1): 80-86. doi: 10.22038/ijbms.2024.79864.17305

Endurance training and pyruvate dehydrogenase kinase 4 (PDK4) inhibition combination is superior to each one alone in attenuating hyperketonemia/ketoacidosis in diabetic rats

Iranian Journal of Basic Medical Sciences
مقاله 10، دوره 28، شماره 1، فروردین 2025، صفحه 80-86    اصل مقاله (366.63 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2024.79864.17305
نویسندگان
Hamed Rezaeinasab1، 2؛ Abdolhamid Habibi1؛ Ramin Rezaei3؛ Aref Basereh4؛ Salva Reverentia Yurista5؛ Kayvan Khoramipour* 6
1Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Shahid Chamran University, Ahvaz, Iran
2Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
3Head of Research and Development Department, Islamic Republic of Iran Basketball Federation
4Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Kharazmi University, Tehran, Iran
5Heart, Vascular and Thoracic Institute, Cleveland Clinic, Cleveland, OH, USA
6i+HeALTH Strategic Research Group, Department of Health Sciences, Miguel de Cervantes European University (UEMC), 47012 Valladolid, Spain
چکیده
Objective(s): While ketone bodies are not the main heart fuel, exercise may increase their uptake. This study aimed to investigate the effect of 6-week endurance training and Pyruvate dehydrogenase kinase 4 )PDK4( inhibition on ketone bodies metabolism in the heart of diabetic rats with emphasis on the role of Peroxisome proliferator-activated receptor-gamma coactivator PGC-1alpha (PGC-1α).
Materials and Methods: Sixty male Wistar rats were divided into eight groups: healthy control group (CONT), endurance training group (TRA), diabetic group (DM), DM + EX group, Dichloroacetate (DCA) group, DM + DCA group, TRA + DCA group, and DM + TRA + DCA group. Diabetes was induced using streptozotocin (STZ). The animals in training groups ran on the treadmill for six weeks (30–50 min running at 20–30 m/min). After the training period, molecular markers for mitochondrial biogenesis and ketone metabolism were assessed in the heart. Circulating ß-hydroxybutyrate (ßOHB) and Acetylacetonate (AcAc) levels were also measured. 
Results: Our results showed that 6-week endurance training increased the cardiac expression of PGC-1α, 3-oxoacid CoA-transferase 1 (OXCT1), and Acetyl-CoA Acetyltransferase 1 (ACAT1) and reduced beta-hydroxybutyrate dehydrogenase1 (BDH1) expression (P≤0.05). In addition, exercise and DCA usage significantly decreased PDK4 gene expression, ßOHB, and AcAc blood levels (P≤0.05). Furthermore, the combination of 6-week endurance training and DCA supplementation led to more reduction in PDFK4 gene expression, ßOHB, and AcAc blood levels. 
Conclusion: Six-week endurance training and DCA supplementation could safely improve ketone body metabolism in the heart, ultimately reducing hyperketonemia/ketoacidosis in diabetic rats. 
کلیدواژه‌ها
Dietary supplements؛ Endurance training؛ Gene expression؛ Ketone bodies؛ Ketosis؛ Pyruvate dehydrogenase - kinase 4
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