سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Jeddi, Farhad, Zahertar, Sara, Bordbar, Ali, Salimnejad, Ramin, Ghobadi, Hassan, Aslani, Mohammad Reza. (1403). Crocin from saffron ameliorates allergic airway inflammation through NF-κB, IL-17, and Nrf2/HO-1 signaling pathways in mice. , 27(12), 1624-1631. doi: 10.22038/ijbms.2024.80614.17447
Farhad Jeddi; Sara Zahertar; Ali Bordbar; Ramin Salimnejad; Hassan Ghobadi; Mohammad Reza Aslani. "Crocin from saffron ameliorates allergic airway inflammation through NF-κB, IL-17, and Nrf2/HO-1 signaling pathways in mice". , 27, 12, 1403, 1624-1631. doi: 10.22038/ijbms.2024.80614.17447
Jeddi, Farhad, Zahertar, Sara, Bordbar, Ali, Salimnejad, Ramin, Ghobadi, Hassan, Aslani, Mohammad Reza. (1403). 'Crocin from saffron ameliorates allergic airway inflammation through NF-κB, IL-17, and Nrf2/HO-1 signaling pathways in mice', , 27(12), pp. 1624-1631. doi: 10.22038/ijbms.2024.80614.17447
Jeddi, Farhad, Zahertar, Sara, Bordbar, Ali, Salimnejad, Ramin, Ghobadi, Hassan, Aslani, Mohammad Reza. Crocin from saffron ameliorates allergic airway inflammation through NF-κB, IL-17, and Nrf2/HO-1 signaling pathways in mice. , 1403; 27(12): 1624-1631. doi: 10.22038/ijbms.2024.80614.17447

Crocin from saffron ameliorates allergic airway inflammation through NF-κB, IL-17, and Nrf2/HO-1 signaling pathways in mice

Iranian Journal of Basic Medical Sciences
مقاله 15، دوره 27، شماره 12، اسفند 2024، صفحه 1624-1631    اصل مقاله (615.81 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2024.80614.17447
نویسندگان
Farhad Jeddi1؛ Sara Zahertar2؛ Ali Bordbar2؛ Ramin Salimnejad3؛ Hassan Ghobadi4؛ Mohammad Reza Aslani* 4، 5
1Department of Genetics and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
2Lung Diseases Research Center, Faculty of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran
3Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
4Lung Diseases Research Center, Ardabil University of Medical Sciences, Ardabil, Iran
5Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): Asthma is a complex inflammatory disorder with the infiltration of inflammatory cells in the lung airways. Saffron’s active component, crocin, has been proven to possess anti-inflammatory and anti-oxidant effects. The objective of this current study was to explore the impact of crocin on NF-kB and nuclear erythroid 2-related factor 2 (Nrf2)/ heme oxygenase-1 (HO-1) signaling pathways in ovalbumin (OVA)-sensitized mice, aiming to understand its mechanism.
Materials and Methods: Four different groups were formed by dividing forty male BALB/C mice: control group, OVA-sensitized group (OVA), OVA combined with crocin 30 mg/kg (OVA-Cr30), and the OVA combined with crocin 60 mg/kg (OVA-Cr60). In order to determine the total number of WBC and inflammatory cells infiltrating the lung, we utilized the bronchoalveolar lavage fluid for counting purposes. The mRNA and protein levels of Nrf2, HO-1, IL-17, and NF-κB in lung tissue were assessed through real-time PCR and western blot techniques. 
Results: Crocin significantly prevented the increase of total WBC and inflammatory cells in the lung tissue (P<0.001 for all) and histopathological changes in OVA-sensitized mice. Furthermore, crocin displayed suppressive effects on the enhancement of NF-kB (P<0.01) and IL-17 (P<0.05) mRNA and protein levels in OVA-sensitized mice while preserving Nrf2 (P<0.01) and HO-1 (P<0.05) expression levels. Crocin effects became increasingly apparent when utilized at high concentrations.
Conclusion: Crocin decreased airway inflammation, partially by inhibiting NF-κB and IL-17 and up-regulating Nrf2/HO-1 mRNA and protein expression levels. 
کلیدواژه‌ها
Asthma؛ Crocin؛ Heme oxygenase-1؛ Nuclear erythroid 2-related - factor 2؛ Ovalbumin
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