Effects of different pre-conditioning exercise on leptin synthesis and its downstream signalling pathway in T2DM rats | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 4، دوره 28، شماره 1، فروردین 2025، صفحه 31-37 اصل مقاله (433.27 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2024.77774.16828 | ||
| نویسندگان | ||
| Sen Lin1؛ Yuzhi Hu2؛ Shuqiao Ding3؛ Yazhe Hu* 3 | ||
| 1School of Sports Science and Technology, Department of Sports Health, Wuhan Sports University, Wuhan, 430079, China | ||
| 2School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, 100029, Beijing, China | ||
| 3School of Physical Education, Department of Sports Health, Central China Normal University, Wuhan, 430079, China | ||
| چکیده | ||
| Objective(s): This study aimed to evaluate the effects of pre-conditioning exercise on body lipid metabolism, leptin secretion, and the downstream pathways at the early stage of type 2 diabetes mellitus (T2DM). Materials and Methods: The T2DM model was established using an 8-week high-sugar, high-fat diet combined. The T2DM model was established using an 8-week high-sugar, high-fat diet combined with streptozocin (STZ) injection. Two exercise interventions, high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) were performed during the model-building process. One week following the STZ injection, rats were euthanized. Blood, gastrocnemius muscle, and epididymal fat pad were collected. Plasma leptin content was measured by ELISA. The expression of leptin-mRNA in epididymal adipose tissue was measured using RT-qPCR, and its protein expression was detected by a western blot. Leptin, leptin-R, and AMPK (AMP-activated protein kinase) - ACC (Acetyl-CoA carboxylase) expression in gastrocnemius muscle was also detected by western blot. Free fatty acids (FFA) and triglycerides (TG) contents in gastrocnemius muscle were measured using a biochemical assay. Results: In the HIIT group, glucose tolerance and leptin receptor expression increased, as did the expression and phosphorylation of AMPK protein. At the early stage of T2DM, it increased significantly in the gastrocnemius muscle in the MICT group. Conclusion: At the early stage of T2DM, pre-conditioning exercise in the form of HIIT was found to inhibit the leptin-mRNA expression in adipose tissue, suppress leptin synthesis, up-regulate AMPK-ACC signaling pathway, and promote lipid decomposition in skeletal muscle tissue. Pre-conditioning of MICT led to the accumulation of FFA and TG in skeletal muscle, likely due to exercise adaptation rather than ectopic deposition of lipids. | ||
| کلیدواژهها | ||
| Diabetes Mellitus؛ High-intensity interval training؛ Leptin؛ Lipid metabolism؛ Sports medicine | ||
| مراجع | ||
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1. Atlas D. International diabetes federation. IDF Diabetes Atlas, 7th edn Brussels, Belgium: International Diabetes Federation 2015; 33: 1-140. 2. D’souza AM, Neumann UH, Glavas MM, Kieffer TJ. The glucoregulatory actions of leptin. Mol Metab 2017; 6: 1052-1065. 3. Oral EA, Simha V, Ruiz E, Andewelt A, Premkumar A, Snell P, et al. Leptin-replacement therapy for lipodystrophy. N Engl J Med 2002; 346: 570-578. 4. Minokoshi Y, Kim Y-B, Peroni OD, Fryer LG, Müller C, Carling D, et al. Leptin stimulates fatty-acid oxidation by activating AMP-activated protein kinase. Nature 2002; 415: 339-343. 5. Chen Y-C, Lee S-D, Ho L-T, Kuo C-H. The effects of altitude training on the AMPK-related glucose transport pathway in the red skeletal muscle of both lean and obese Zucker rats. High Alt Med Biol 2011; 12: 371-378. 6. Park KG, Min AK, Koh EH, Kim HS, Kim MO, Park HS, et al. Alpha‐lipoic acid decreases hepatic lipogenesis through adenosine monophosphate‐activated protein kinase (AMPK)‐dependent and AMPK‐independent pathways. Hepatology 2008; 48: 1477-1486. 7. Musi N, Fujii N, Hirshman MF, Ekberg I, Froberg S, Ljungqvist O, et al. AMP-activated protein kinase (AMPK) is activated in muscle of subjects with type 2 diabetes during exercise. Diabetes 2001; 50: 921-927. 8. Cao S, Li B, Yi X, Chang B, Zhu B, Lian Z, et al. Effects of exercise on AMPK signaling and downstream components to PI3K in rat with type 2 diabetes. PLoS One 2012; 7: e51709-51721. 9. Yi X, Cao S, Chang B, Zhao D, Gao H, Wan Y, et al. Effects of acute exercise and chronic exercise on the liver leptin‐AMPK‐ACC signaling pathway in rats with type 2 diabetes. J Diabetes Res 2013; 2013: 946432-946440. 10. Jelleyman C, Yates T, O’Donovan G, Gray LJ, King JA, Khunti K, et al. The effects of high‐intensity interval training on glucose regulation and insulin resistance: A meta‐analysis. Obes Rev 2015; 16: 942-961. 11. Jung ME, Bourne JE, Beauchamp MR, Robinson E, Little JP. High‐intensity interval training as an efficacious alternative to moderate‐intensity continuous training for adults with prediabetes. J Diabetes Res 2015; 2015: 191595-191603. 12. Li F-H, Li T, Ai J-Y, Sun L, Min Z, Duan R, et al. Beneficial autophagic activities, mitochondrial function, and metabolic phenotype adaptations promoted by high-intensity interval training in a rat model. Front Physiol 2018; 9:571-585. 13. Kanter M, Coskun O, Korkmaz A, Oter S. Effects of Nigella sativa on oxidative stress and β‐cell damage in streptozotocin‐induced diabetic rats. Anat Rec A Discov Mol Cell Evol Biol 2004; 279: 685-691. 14. Gao X, Zhou Y. Review of mouse and rat models for type 2 diabetes mellitus. Acta Lab Anim Sci Sin 2014; 22: 71-76. 15. Bouassida A, Zalleg D, Bouassida S, Zaouali M, Feki Y, Zbidi A, et al. Leptin, its implication in physical exercise and training: A short review. J Sports Sci Med 2006; 5:172-181. 16. Maeda H, Hosokawa M, Sashima T, Miyashita K. Dietary combination of fucoxanthin and fish oil attenuates the weight gain of white adipose tissue and decreases blood glucose in obese/diabetic KK-Ay mice. J Agric Food Chem 2007; 55:7701-7706. 17. Drevon CA. Fatty acids and expression of adipokines. Biochim Biophys Acta 2005; 1740: 287-292. 18. Tilg H, Moschen AR. Adipocytokines: Mediators linking adipose tissue, inflammation and immunity. Nat Rev Immunol 2006; 6: 772-783. 19. Pratley R, Ren K, Milner M, Sell S. Insulin increases leptin mRNA expression in abdominal subcutaneous adipose tissue in humans. Mol Genet Metab 2000; 70: 19-26. 20. Li J-h, Zhang M, Zhang Z-d, Pan X-h, Pan L-l, Sun J. GPR41 deficiency aggravates type 1 diabetes in streptozotocin-treated mice by promoting dendritic cell maturation. Acta Pharmacol Sin 2024; 45: 1466-1476. 21. Adhikary P, Banerjee S, Dey BK, Gargari P, Chatterjee S, Chakraborty D, et al. Association of adipocyte size and SREBP-1c in visceral and subcutaneous adipose tissue in non-obese type 2 diabetes mellitus. Endocrine 2024; 83: 615-623. 22. Wang J, Liu R, Hawkins M, Barzilai N, Rossetti L. A nutrient-sensing pathway regulates leptin gene expression in muscle and fat. Nature 1998; 393: 684-688. 23. Ramsay T, Richards M. Leptin and leptin receptor expression in skeletal muscle and adipose tissue in response to in vivo porcine somatotropin treatment. J Anim Sci 2005; 83: 2501-2508. 24. Wolsk E, Mygind H, Grøndahl TS, Pedersen BK, van Hall G. Human skeletal muscle releases leptin in vivo. Cytokine 2012; 60: 667-673. 25. Kjøbsted R, Hingst JR, Fentz J, Foretz M, Sanz M-N, Pehmøller C, et al. AMPK in skeletal muscle function and metabolism. FASEB J 2018; 32:1741-1777. 26. Kang S, Kim KB, Shin KO. Exercise training improve leptin sensitivity in peripheral tissue of obese rats. Biochem Biophys Res Commun 2013; 435: 454-459. 27. Steinberg GR, Dyck DJ. Development of leptin resistance in rat soleus muscle in response to high-fat diets. Am J Physiol Endocrinol Metab 2000; 279: E1374-E1382. 28. Cochran AJ, Percival ME, Tricarico S, Little JP, Cermak N, Gillen JB, et al. Intermittent and continuous high‐intensity exercise training induce similar acute but different chronic muscle adaptations. Exp Physiol 2014; 99: 782-791. 29. Martinez‐Huenchullan SF, Maharjan BR, Williams PF, Tam CS, Mclennan SV, Twigg SM. Differential metabolic effects of constant moderate versus high intensity interval training in high‐fat fed mice: Possible role of muscle adiponectin. Physiol Rep 2018; 6: e13599-13614. 30. Burkewitz K, Zhang Y, Mair WB. AMPK at the nexus of energetics and aging. Cell Metab 2014; 20:10-25. 31. Torma F, Gombos Z, Jokai M, Takeda M, Mimura T, Radak Z. High intensity interval training and molecular adaptive response of skeletal muscle. Sports Med Health Sci 2019; 1: 24-32. 32. Şakul A, Cumaoğlu A, Aydın E, Arı N, Dilsiz N, Karasu Ç. Age-and diabetes-induced regulation of oxidative protein modification in rat brain and peripheral tissues: Consequences of treatment with antioxidant pyridoindole. Exp Gerontol 2013; 48: 476-484. 33. Sjöros T, Saunavaara V, Löyttyniemi E, Koivumäki M, Heinonen IH, Eskelinen JJ, et al. Intramyocellular lipid accumulation after sprint interval and moderate‐intensity continuous training in healthy and diabetic subjects. Physiol Rep 2019; 7:e13980-13990. 34. Carraro U, Franceschi C. Apoptosis of skeletal and cardiac muscles and physical exercise. Aging 1997; 9: 19-34. | ||
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