سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Mohammadpour, Amir Hooshang, Ramezani, Mohammad, Tavakoli Anaraki, Nasim, Malaekeh-Nikouei, Bizhan, Amel Farzad2, Sara, Hosseinzadeh, Hossein. (1391). Development and Validation of HPLC Method for Determination of Crocetin, a constituent of Saffron, in Human Serum Samples. , 16(1), 47-56. doi: 10.22038/ijbms.2013.248
Amir Hooshang Mohammadpour; Mohammad Ramezani; Nasim Tavakoli Anaraki; Bizhan Malaekeh-Nikouei; Sara Amel Farzad2; Hossein Hosseinzadeh. "Development and Validation of HPLC Method for Determination of Crocetin, a constituent of Saffron, in Human Serum Samples". , 16, 1, 1391, 47-56. doi: 10.22038/ijbms.2013.248
Mohammadpour, Amir Hooshang, Ramezani, Mohammad, Tavakoli Anaraki, Nasim, Malaekeh-Nikouei, Bizhan, Amel Farzad2, Sara, Hosseinzadeh, Hossein. (1391). 'Development and Validation of HPLC Method for Determination of Crocetin, a constituent of Saffron, in Human Serum Samples', , 16(1), pp. 47-56. doi: 10.22038/ijbms.2013.248
Mohammadpour, Amir Hooshang, Ramezani, Mohammad, Tavakoli Anaraki, Nasim, Malaekeh-Nikouei, Bizhan, Amel Farzad2, Sara, Hosseinzadeh, Hossein. Development and Validation of HPLC Method for Determination of Crocetin, a constituent of Saffron, in Human Serum Samples. , 1391; 16(1): 47-56. doi: 10.22038/ijbms.2013.248

Development and Validation of HPLC Method for Determination of Crocetin, a constituent of Saffron, in Human Serum Samples

Iranian Journal of Basic Medical Sciences
مقاله 5، دوره 16، شماره 1، فروردین 2013، صفحه 47-56    اصل مقاله (429.57 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2013.248
نویسندگان
Amir Hooshang Mohammadpour1؛ Mohammad Ramezani2؛ Nasim Tavakoli Anaraki3؛ Bizhan Malaekeh-Nikouei4؛ Sara Amel Farzad22؛ Hossein Hosseinzadeh* 3
1Pharmaceutical Research Center, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
22Pharmaceutical Research Centre, Department of Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
31Pharmaceutical Research Center, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
43Nanotechnology Research Centre, Department of Pharmaceutics, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s):The present study reports the development and validation of a sensitive and rapid extraction method beside high performance liquid chromatographic method for the determination of crocetin in human serum.
Materials and Methods:The HPLC method was carried out by using a C18 reversed-phase column and a mobile phase composed of methanol/water/acetic acid (85:14.5:0.5 v/v/v) at the flow rate of 0.8 ml/min. The UV detector was set at 423 nm and 13-cis retinoic acid was used as the internal standard. Serum samples were pretreated with solid-phase extraction using Bond Elut C18 (200mg) cartridges or with direct precipitation using acetonitrile.
Results:The calibration curves were linear over the range of 0.05-1.25 µg/ml for direct precipitation method and 0.5-5 µg/ml for solid-phase extraction. The mean recoveries of crocetin over a concentration range of 0.05-5 µg/ml serum for direct precipitation method and 0.5-5 µg/ml for solid-phase extraction were above 70 % and 60 %, respectively. The intraday coefficients of variation were 0.37- 2.6% for direct precipitation method and 0.64 - 5.43% for solid-phase extraction. The inter day coefficients of variation were 1.69 – 6.03% for direct precipitation method and 5.13-12.74% for solid-phase extraction, respectively. The lower limit of quantification for crocetin was 0.05 µg/ml for direct precipitation method and 0.5 µg/ml for solid-phase extraction.
Conclusion: The validated direct precipitation method for HPLC satisfied all of the criteria that were necessary for a bioanalytical method and could reliably quantitate crocetin in human serum for future clinical pharmacokinetic study
کلیدواژه‌ها
Crocetin؛ Crocus sativus؛ Direct precipitation؛ High Performance Liquid Chromatography؛ Human serum samples؛ Saffron؛ Solid phase extraction
مراجع
  1. Schmidt M, Betti G, Hensel A. Saffron in phytotherapy: pharmacology and clinical uses. Wien Med Wochenschr2007; 157:315-319.
  2. Hosseinzadeh H, Shamsaie F, Mehri S: Antioxidant activity of aqueous and ethanolic extracts of Crocus sativus L. stigma and its bioactive constituents cracin and safranal. Pharmacogn Mag 2010; 5:419-424.
  3. Hosseinzadeh H, Khosravan V. Anticonvulsant effects of aqueous and ethanolic extracts of Crocus sativus L., stigmas in mice. Arch Iran Med 2002; 5:44-47.
  4. Hosseinzadeh H, Talebzadeh F. Anticonvulsant evaluation of safranal and crocin from Crocus sativus in mice. Fitoterapia 2005; 76:722-724.
  5. Hosseinzadeh H, Sadeghnia HR, Rahimi A. Effect of safranal on extracellular hippocampal levels of glutamate and aspartate during kainic Acid treatment in anesthetized rats. Planta Med 2008; 74:1441-1445.
  6. Sadeghnia HR, Cortez MA, Liu D, Hosseinzadeh H, Snead OC 3rd. Antiabsence effects of safranal in acute experimental seizure models: EEG and autoradiography. J Pharm Pharm Sci 2008; 11:1-14.
  7. Hosseinzadeh H, Sadeghnia HR. Protective effect of safranal on pentylenetetrazol-induced seizures in the rat: involvement of GABAergic and opioids systems. Phytomedicine 2007; 14:256-262.
  8. Hosseinzadeh H, Younesi HM. Antinociceptive and anti-inflammatory effects of Crocus sativus L., stigma and petal extracts in mice. BMC Pharmacol 2002; 2:7.
  9. Amin B, Hosseinzadeh H. Evaluation of aqueous and ethanolic extracts of saffron, Crocus sativus L., and its constituents, safranal and crocin in allodynia and hyperalgesia induced by chronic constriction injury model of neuropathic pain in rats. Fitoterapia 2012; 83:888-893.
  10.  Hosseinzadeh H, Shariaty VM. Anti-nociceptive effect of safranal, a constituent of Crocus sativus (saffron), in mice.Pharmacologyonline2007; 2:498-503.
  11. Golmohammadzadeh S, Jaafari MR, Hosseinzadeh H. Does saffron have antisolar and moisturizing effects? Iran J Pharmac Res 2010; 9:133-140.
  12.  Golmohammadzadeh S, Imani F, Hosseinzadeh H, Jaafari MR. Preparation, characterization and evaluation of sun protective and moisturizing effects of nanoliposomes containing safranal. Iran J Basic Med Sci  2011;14:521-533.
  13.  Mehri S, Abnous K, Mousavi SH, Shariaty VM, Hosseinzadeh H. Neuroprotective effect of crocin on acrylamide-induced cytotoxicity in PC12 cells. Cell Mol Neurobiol 2012; 32:227-235.
  14.  Hariri AT, Moallem SA, Mahmoudi M, Hosseinzadeh H. The effect of crocin and safranal, constituents of saffron, against subacute effect of diazinon on hematological and genotoxicity indices in rats. Phytomedicine  2011; 18:499-504.
  15.  Hariri AT, Moallem SA, Mahmoudi M, Memar B, Hosseinzadeh H. Sub-acute effects of diazinon on biochemical indices and specific biomarkers in rats: Protective effects of crocin and safranal. Food Chem Toxicol 2010; 48:2803-2808.
  16.  Naghizadeh B, Mansouri SMT, Mashhadian NV. Crocin attenuates cisplatin-induced renal oxidative stress in rats. Food Chem Toxicol 2010; 48:2650-2655.
  17.  Hosseinzadeh H, Sadeghnia HR. Effect of safranal, a constituent of Crocus sativus (saffron), on methyl methanesulfonate (MMS)-induced DNA damage in mouse organs: an alkaline single-cell gel electrophoresis (comet) assay. DNA Cell Biol  2007; 26:841-846.
  18.  Hosseinzadeh H, Abootorabi A, Sadeghnia HR. Protective effect of Crocus sativus stigma extract and crocin (trans-crocin 4) on methyl methanesulfonate-induced DNA damage in mice organs. DNA Cell Biol 2008; 27:657-664.
  19.  Shamsa A, Hosseinzadeh H, Molaei M, Shakeri MT, Rajabi O. Evaluation of Crocus sativus L. (saffron) on male erectile dysfunction: a pilot study. Phytomedicine 2009; 16: 690-693.
  20.  Hosseinzadeh H, Ziaee T, Sadeghi A. The effect of saffron, Crocus sativus stigma, extract and its constituents, safranal and crocin on sexual behaviors in normal male rats. Phytomedicine 2008; 15:491-495.
  21.  Abdullaev FI,  Caballero-Ortega H,  Riveron-Negrette L, Pereda-Miranda R, Hernandez MJ, Perez-Lopez J. Use of in vitro assays to assess the potential antigenotoxic and cytotoxic effects of saffron (Crocus sativus L.). Toxicol In Vitro 2003; 17:731–736.
  22.  Ichi T, Higashimura Y, Katayama T, Shimadzu T, Tada M. Analysis of crocetin derivatives from gardenia (Gardenia jasminoides Ellis) fruits. J Jpn Soc Food Sci Technol 1995; 42:76-783.
  23.  Rio JL, Recio MC, Ginger RM, Manez S. An update review of saffron and its active constituents. Phytother Res 1996; 10:189-193.
  24.  Xuan B, Zhou YH, Li N, Min ZD, Chiou GC. Effects of crocin analogs on ocular blood flow and retinal function. J Ocul Pharmacol Ther 1999; 15:143-152.
  25.  Giaccio M. Crocetin from saffron: An active component of an ancient spice. Crit Rev Food Sci Nutr 2004; 44:155-172.
  26. Wang CJ, Lee MJ, Chang MC, Lin JK. Inhibition of tumor promotion in benzo (a) pyrene-initiated CD-1 mouse skin by crocetin. Carcinogenesis 1995; 16:187-191.
  27.  Tseng TH, Chu CY, Huang JM, Shiow SJ, Wang CJ. Crocetin protects against oxidative damage in rat primary hepatocytes. Cancer Lett 1995; 97:61–67.
  28.  He SY, Qian ZY, Wen N, Tang F, Xu GL, Zhou C. Influence of crocetin on experimental atherosclerosis in hyperlipidamic-diet quails. Eur J Pharmacol 2007; 554: 191-195.
  29.  Sujatha V, Ravishankar G, Venkataraman V. Methods for the analysis of the saffron metabolites crocin, crocetins, picrocrocin and safranal for the determination of the quality of the spice using thin-layer chromatography, high-performance liquid chromatography and gas chromatography. J Chromatogr 1992; 624:497-502.
  30.  Liu T, Qian ZY. Pharmacokinetics of crocetin in rats. Yao Xue Xue Bao 2002; 37:367-369.
  31.  Asai A, Nakano T, Takahashi M, Nagao A. Orally administered crocetin and crocins are absorbed into blood plasma as crocetin and its glucuronide conjugates in mice. J Agric Food Chem 2005; 53:7302-7306.
  32.  Xi L, Qian Z, Du P, Fu J. Pharmacokinetic properties of crocin(crocetin digentiobiose ester) following oral administration in rats. Phytomedicine 2007; 14:633-636.
  33.  Venn RF. Principles and Practice of Bioanalysis. 1st ed.Taylor & Francis, NY, USA: 2000.
  34.  ICH QB. Analytical Procedures and Methods Validation Chemistry, Manufacturing, and Controls Documentation, 1996.
  35.  Zheng S, Qian Z, Tang F, Sheng L. Suppression of vascular cell adhesion molecule-1 expression by crocetin contributes to attenuation of atherosclerosis in hypercholesterolemic rabbits. Biochem Pharmacol 2005; 70:1192–1199.
  36.  Umigai N, Murakami K, Ulit MV, Antonio LS, Shirotori M, Morikawa H, et al. The pharmacokinetic profile of crocetin in healthy adult human volunteers after a single oral administration. Phytomedicine 2011; 18:575-578.
  37.  Chryssanthi DG, Lamari FN, Georgakopoulos CD, Cordopatis P. A new validated SPE-HPLC method for monitoring crocetin in human plasma-Application after saffron tea consumption. J Pharm Biomed Anal 2011; 55:563-568.
آمار
تعداد مشاهده مقاله: 3,775
تعداد دریافت فایل اصل مقاله: 1,672