Beta-adrenergic receptor stimulation, histamine receptor inhibition, and potassium channel opening contribute to the relaxant effects of crocetin on airway smooth muscle | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 12، دوره 27، شماره 10، دی 2024، صفحه 1317-1322 اصل مقاله (376.88 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2024.77720.16822 | ||
| نویسندگان | ||
| Sepide Behrouz1، 2؛ Arghavan Memarzia1، 2؛ Mohammad Hossein Eshaghi Ghalibaf1، 2، 3؛ Mohammad Hossein Boskabady* 1، 2 | ||
| 1Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 2Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 3Saffron institute, University of Torbat Heydariyeh, Torbat Heydariyeh, Iran | ||
| چکیده | ||
| Objective(s): In the present study, the relaxant effect of crocetin on tracheal smooth muscle cells (TSM) and its possible mechanisms were evaluated. Materials and Methods: The study was conducted on 54 male Wistar rats in 8 groups. TSM was contracted by methacholine (10 μM) and KCl (60 mM), and the relaxant effects of four cumulative concentrations of crocetin, petal extract of saffron, and theophylline were examined on non-incubated and TSM incubated with propranolol, chlorpheniramine, diltiazem, atropine, glibenclamide, and indomethacin were investigated. Results: In non-incubated TSM contracted by methacholine or KCl, crocetin and theophylline showed concentration-dependent relaxant effects (all, P<0.001). However, various concentrations of crocetin showed significantly lower relaxant effects compared to those of theophylline (all, P<0.001). In the methacholine-induced contraction of TSM, the relaxation effect of the last concentration of crocetin in the TSM incubated with propranolol was lower than in non-incubated TSM (P<0.05). In the incubated TSM with chlorpheniramine, the relaxant effects of the two last concentrations of crocetin were significantly lower than in the non-incubated tissues contracted by KCl (P<0.05 and P<0.0). The levels of EC50 crocetin in the incubated TSM with glibenclamide, chlorpheniramine, and indomethacin were markedly lower than in non-incubated (all, P<0.05). Conclusion: The results showed potent relaxation effects of crocetin on TSM and were suggested to be through stimulation of ß-adrenergic receptors, inhibition of histamine (H1) receptors, and potassium channel opening mechanisms. | ||
| کلیدواژهها | ||
| Airway smooth muscle؛ Crocetin؛ Cyclooxygenase؛ Histamine receptors؛ Potassium channels؛ Relaxant effects | ||
| مراجع | ||
|
1. Bousquet J, Jeffery PK, Busse WW, Johnson M, Vignola AM. Asthma: from bronchoconstriction to airways inflammation and remodeling. Am J Respir Crit Care Med 2000;161:1720-1745. 2. Bergeron C, Tulic MK, Hamid Q. Airway remodelling in asthma: From benchside to clinical practice. Can Respir J 2010;17:e85-93. 3. Brutsche MH, Downs SH, Schindler C, Gerbase MW, Schwartz J, Frey M, et al. Bronchial hyperresponsiveness and the development of asthma and COPD in asymptomatic individuals: SAPALDIA cohort study. Thorax 2006;61:671-677. 4. Doeing DC, Solway J. Airway smooth muscle in the pathophysiology and treatment of asthma. J Appl Physiol 2013;114:834-843. 5. Laitinen A, Partanen M, Hervonen A, Laitinen LA. Electron microscopic study on the innervation of the human lower respiratory tract: evidence of adrenergic nerves. Eur J Respir Dis 1985;67:209-215. 6. Van der Velden VH, Hulsmann AR. Autonomic innervation of human airways: structure, function, and pathophysiology in asthma. Neuroimmunomodulation 1999;6:145-159. 7. Corry DB. Emerging immune targets for the therapy of allergic asthma. Nat Rev Drug Discov 2002;1:55-64. 8. Taur DJ, Patil RY. Some medicinal plants with antiasthmatic potential: A current status. Asian Pac J Trop Biomed 2011;1:413-418. 9. Greenberger PA. Therapy in the management of the rhinitis/asthma complex. Allergy Asthma Proc 2003;24:403-407. 10. Javadi B, Sahebkar A, Emami SA. A survey on saffron in major islamic traditional medicine books. Iran J Basic Med Sci 2013;16:1-11. 11. Sadr S, Kaveh N, Agin K, Choopani R, Kaveh S, Tahermohammadi H. Herbal treatments for Asthma, according to Avicenna. Int J Pediatr 2022;10:15205-15226. 12. Wang X, Zhang G, Qiao Y, Feng C, Zhao X. Crocetin attenuates spared nerve injury-induced neuropathic pain in mice. J Pharmacol Sci 2017;135:141-147. 13. Nam KN, Park YM, Jung HJ, Lee JY, Min BD, Park SU, et al. Anti-inflammatory effects of crocin and crocetin in rat brain microglial cells. Eur J Pharmacol 2010;648:110-116. 14. Moradzadeh M, Sadeghnia HR, Tabarraei A, Sahebkar A. Anti-tumor effects of crocetin and related molecular targets. J Cell Physiol 2018;233:2170-2182. 15. Ding J, Su J, Zhang L, Ma J. Crocetin activates Foxp3 through TIPE2 in asthma-associated treg cells. Cell Physiol Biochem 2015;37:2425-2433. 16. Magesh V. Effect of crocetin on benzo (a) pyrene induced lung carcinogenesis in swiss albino mice venkatraman magesh, ramachandran venugopal, konga durga bhavani and dhanapal sakthisekaran. Int J Cancer Res 2007;3:143-150. 17. Ding Y, Ma L, He L, Xu Q, Zhang Z, Zhang Z, et al. A strategy for attenuation of acute radiation-induced lung injury using crocetin from gardenia fruit. Biomed Pharmacother 2022;149:112899. 18. Yang R, Yang L, Shen X, Cheng W, Zhao B, Ali KH, et al. Suppression of NF-κB pathway by crocetin contributes to attenuation of lipopolysaccharide-induced acute lung injury in mice. Eur J Pharmacol 2012;674:391-396. 19. Llorens S, Mancini A, Serrano-Díaz J, D’Alessandro AM, Nava E, Alonso GL, et al. Effects of crocetin esters and crocetin from Crocus sativus L. on aortic contractility in rat genetic hypertension. Molecules 2015;20:17570-84. 20. Saadat S, Boskabadi J, Boskabady MH. Contribution of potassium channels, beta2-adrenergic and histamine H1 receptors in the relaxant effect of baicalein on rat tracheal smooth muscle. Iran J Basic Med Sci 2019;22:1347-1352. 21. Memarzia A, Amin F, Saadat S, Jalali M, Ghasemi Z, Boskabady MH. The contribution of beta-2 adrenergic, muscarinic and histamine (H1) receptors, calcium and potassium channels and cyclooxygenase pathway in the relaxant effect of Allium cepa L. on the tracheal smooth muscle. J Ethnopharmacol 2019;241:112012. 22. Malerba M, Radaeli A, Mancuso S, Polosa R. The potential therapeutic role of potassium channel modulators in asthma and chronic obstructive pulmonary disease. J Biol Regul Homeost Agents 2010;24:123-130. 23. Saeideh S, Yasavoli M, Gholamnezhad Z, Aslani MR, Boskabady MH. The relaxant effect of crocin on rat tracheal smooth muscle and its possible mechanisms. Iran J Pharm Res 2019;18:1358-1370. 24. Zhao Z, Zheng B, Li J, Wei Z, Chu S, Han X, et al. Influence of crocetin, a natural carotenoid dicarboxylic acid in saffron, on L-Type Ca(2+) current, intracellular Ca(2+) handling and contraction of isolated rat cardiomyocytes. Biol Pharm Bull 2020;43:1367-1374. 25. White MV. The role of histamine in allergic diseases. J Allergy Clin Immunol. 1990;86:599-605. 26. Yamauchi K, Ogasawara M. The role of histamine in the pathophysiology of asthma and the clinical efficacy of antihistamines in asthma therapy. Int J Mol Sci 2019;20:1733. 27. Yamauchi K, Shikanai T, Nakamura Y, Kobayashi H, Ogasawara M, Maeyama K. Roles of histamine in the pathogenesis of bronchial asthma and reevaluation of the clinical usefulness of antihistamines. Yakugaku Zasshi 2011;131:185-191. 28. Boskabady M, Rahbardar MG, Nemati H, Esmaeilzadeh M. Inhibitory effect of Crocus sativus (saffron) on histamine (H1) receptors of guinea pig tracheal chains. Int J Pharm Sci 2010;65:300-305. 29. Boskabady MH, Rahbardar MG, Jafari Z. The effect of safranal on histamine (H1) receptors of guinea pig tracheal chains. Fitoterapia 2011;82:162-167. 30. Boskabady MH, Aslani MR. Relaxant effect of Crocus sativus (saffron) on guinea‐pig tracheal chains and its possible mechanisms. J Pharm Pharmacol 2006;58:1385-1390. 31. Demoly P, Crampette L, Lebel B, Campbell AM, Mondain M, Bousquet J. Expression of cyclo-oxygenase 1 and 2 proteins in upper respiratory mucosa. Clin Exp Allergy 1998;28:278-283. 32. Kakuyama M, Ahluwalia A, Rodrigo J, Vallance P. Cholinergic contraction is altered in nNOS knockouts. Cooperative modulation of neural bronchoconstriction by nNOS and COX. Am J Respir Crit Care Med 1999;160:2072-2078. 33. Kowalski ML. Aspirin sensitive rhinosinusitis and asthma. Allergy Proc 1995;16:77-80. 34. Gosens R, Zaagsma J, Meurs H, Halayko AJ. Muscarinic receptor signaling in the pathophysiology of asthma and COPD. Respir Res 2006;7:73-87. 35. Neamati N, Boskabady MH. Effect of Crocus sativus (saffron) on muscarinic receptors of guinea pig tracheal chains. Func Plant Sci Biotec 2010;4:128-131. 36. Higashino S, Sasaki Y, Giddings JC, Hyodo K, Sakata SF, Matsuda K, et al. Crocetin, a carotenoid from Gardenia jasminoides Ellis, protects against hypertension and cerebral thrombogenesis in stroke-prone spontaneously hypertensive rats. Phytother Res. 2014;28:1315-1319. | ||
|
آمار تعداد مشاهده مقاله: 156 تعداد دریافت فایل اصل مقاله: 179 |
||