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Zhou, Honglong, Wang, Ji, Zhao, Peng, Le, Dongsheng, Cai, Shanshan, Mao, Guohua. (1403). Intravenous administration exosomes derived from human amniotic mesenchymal stem cells improves neurological recovery after acute traumatic spinal cord injury in rats. , 27(10), 1284-1292. doi: 10.22038/ijbms.2024.76532.16576
Honglong Zhou; Ji Wang; Peng Zhao; Dongsheng Le; Shanshan Cai; Guohua Mao. "Intravenous administration exosomes derived from human amniotic mesenchymal stem cells improves neurological recovery after acute traumatic spinal cord injury in rats". , 27, 10, 1403, 1284-1292. doi: 10.22038/ijbms.2024.76532.16576
Zhou, Honglong, Wang, Ji, Zhao, Peng, Le, Dongsheng, Cai, Shanshan, Mao, Guohua. (1403). 'Intravenous administration exosomes derived from human amniotic mesenchymal stem cells improves neurological recovery after acute traumatic spinal cord injury in rats', , 27(10), pp. 1284-1292. doi: 10.22038/ijbms.2024.76532.16576
Zhou, Honglong, Wang, Ji, Zhao, Peng, Le, Dongsheng, Cai, Shanshan, Mao, Guohua. Intravenous administration exosomes derived from human amniotic mesenchymal stem cells improves neurological recovery after acute traumatic spinal cord injury in rats. , 1403; 27(10): 1284-1292. doi: 10.22038/ijbms.2024.76532.16576

Intravenous administration exosomes derived from human amniotic mesenchymal stem cells improves neurological recovery after acute traumatic spinal cord injury in rats

Iranian Journal of Basic Medical Sciences
مقاله 8، دوره 27، شماره 10، دی 2024، صفحه 1284-1292    اصل مقاله (1.01 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2024.76532.16576
نویسندگان
Honglong Zhou1؛ Ji Wang2؛ Peng Zhao3؛ Dongsheng Le1؛ Shanshan Cai* 4؛ Guohua Mao* 1
1Department of Neurosurgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China
2Department of Neurosurgery, Institute of Neuroscience, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China
3Interventional Department, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China
4Department of Health Insurance, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China
چکیده
Objective(s): Our previous study has showed that human amniotic mesenchymal stem cells (hAMSCs) transplantation improves neurological recovery after traumatic spinal cord injury (TSCI) in rats. However, less is known about the effects of exosomes derived from hAMSCs for TSCI. Here, we investigated whether hAMSCs-derived exosomes improve neurological recovery in TSCI rats and the underlying mechanisms. 
Materials and Methods: A rat traumatic spinal cord injury (TSCI) mode was established using a weight drop device. At 2 hr after TSCI, rats were administered either hAMSCs-derived exosomes or phosphate buffered saline via the tail vein. Locomotor recovery was evaluated by an open-field locomotor rating scale and gridwalk task. Spinal cord water content, hematoxylin and eosin (H&E) staining, Evans blue (EB) dye extravasation, immunofluorescence staining, and enzyme-linked immunosorbent were performed to elucidate the underlying mechanism.
Results: hAMSCs-derived exosomes significantly reduced the numbers of ED1+ macrophages/microglia and caspase-3+cells and decreased the levels of reactive oxygen species, myeloperoxidase activity and inflammatory cytokines, such as tumor necrosis factor alpha, interleukin-6 and interleukin-1β. In addition, hAMSCs-derived exosomes significantly attenuated spinal cord water content and Evans blue extravasation, and enhanced angiogenesis and axonal regeneration. Finally, hAMSCs-derived exosomes also significantly reduced the lesion volume, inhibited astrogliosis, and improved functional recovery. 
Conclusion: Taken together, these findings demonstrate that hAMSCs-derived exosomes have favourable effects on rats after acute TSCI, and that they may serve as an alternative cell-free therapeutic approach for treating acute TSCI.
کلیدواژه‌ها
Angiogenesis؛ Exosomes؛ Mesenchymal stem cells؛ Neural regeneration؛ Recovery of function
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